Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilY1   Type   Machinery gene
Locus tag   ACK8DE_RS17700 Genome accession   NZ_CP179872
Coordinates   3657042..3660896 (-) Length   1284 a.a.
NCBI ID   WP_023896142.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain MRSN15116     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3652042..3665896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8DE_RS17665 (ACK8DE_17665) - 3652803..3653744 (+) 942 WP_098713316.1 lipase family alpha/beta hydrolase -
  ACK8DE_RS17670 (ACK8DE_17670) rplS 3653797..3654165 (-) 369 WP_000014562.1 50S ribosomal protein L19 -
  ACK8DE_RS17675 (ACK8DE_17675) trmD 3654373..3655113 (-) 741 WP_000464599.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ACK8DE_RS17680 (ACK8DE_17680) rimM 3655159..3655707 (-) 549 WP_005130741.1 ribosome maturation factor RimM -
  ACK8DE_RS17685 (ACK8DE_17685) rpsP 3655727..3655978 (-) 252 WP_000260334.1 30S ribosomal protein S16 -
  ACK8DE_RS17690 (ACK8DE_17690) pilE 3656125..3656550 (-) 426 WP_005130737.1 type IV pilin protein Machinery gene
  ACK8DE_RS17695 (ACK8DE_17695) pilY2 3656547..3657029 (-) 483 WP_001046413.1 type IV pilin protein Machinery gene
  ACK8DE_RS17700 (ACK8DE_17700) pilY1 3657042..3660896 (-) 3855 WP_023896142.1 PilC/PilY family type IV pilus protein Machinery gene
  ACK8DE_RS17705 (ACK8DE_17705) pilX 3660908..3661726 (-) 819 WP_000149377.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  ACK8DE_RS17710 (ACK8DE_17710) pilW 3661723..3662724 (-) 1002 WP_000079192.1 PilW family protein Machinery gene
  ACK8DE_RS17715 (ACK8DE_17715) pilV 3662725..3663285 (-) 561 WP_002194578.1 type IV pilus modification protein PilV Machinery gene
  ACK8DE_RS17720 (ACK8DE_17720) fimU 3663279..3663752 (-) 474 WP_001214067.1 Tfp pilus assembly protein FimT/FimU Machinery gene
  ACK8DE_RS17725 (ACK8DE_17725) ispH 3663922..3664872 (-) 951 WP_000407064.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ACK8DE_RS17730 (ACK8DE_17730) gmk 3664995..3665624 (+) 630 WP_000015937.1 guanylate kinase -

Sequence

Protein


Download         Length: 1284 a.a.        Molecular weight: 138038.48 Da        Isoelectric Point: 7.7713

>NTDB_id=974230 ACK8DE_RS17700 WP_023896142.1 3657042..3660896(-) (pilY1) [Acinetobacter baumannii strain MRSN15116]
MKLKLKNFKPNNLWYAVYSSSMIFTWLMTSSVVQASDLQIYASPTAGKKTIVMMLDTSGSMTNNSYGENRLAMLKNGMNA
FLASNNPVLNDTRVGLGNFSANGDSRSGQILVAAAPLGDASTLNTVGSQRYKLKQAVANLTAGGSTPSAHAYAEAAAYLM
GTTTYSETNYAIRKDSYIKRVRRSDNRTEYSYCTNYRDSQIDTANLWQPCRSNSYWSSWSTNNPGVDNATAYDTSSDWTY
YYTYYYTTFNYAVANADSGIPKSKSNDTASNPNIVVDRNATNSNAVYQSPLPAVANRQSCDGQGIYFLSDGEPNNTTNTR
SASVMSTALGSTFGADFNCSGGLSNTTADSGWACMGEFAKRLFDKTKNPAGVSIQTAFVGFGSDFSSLSSSDVKNACRLS
SRTQSDRKGDDACSPNQSTNAVAAPGYGNGGFFPTQSSQGVTDSVIAFINNLDKVPLEPLTTGAISVPYDALNPKNLQEY
GYLRAFEPNPANTYLTWRGNLKKYHVVLSGANAGAFEANSGGLVYNASGAFRTGTKDYWNSSTYTDGGKVFLGGSYANVP
LPIAGQPETRDAEGNITKYYYAVQSKIRNLFTDVSAVAADGSLTKISTSGTNLLKIPAAPPEGTNPFDTVANTASYVLGK
FDPSTGQNILKAFPISLKLKILNYLGYSTDINATTLPSSLVTSNEPYLSMGGSIHSLPVQLTYNGTLDDNGNLTSAREQS
ILYGTMEGGLHIVDASSGIEQMVFVPADILNDSVASKALVVGQSDASAPAHGMDGAWVSDPAYNITTVGSGSSAVSKVTA
KQMNIYGGMRMGGSSYYGLDVLSPTSPKLLFRIGADQTDYSRMGQSWSKPVLANIRYNGSIRRVLIVGGGYDQCYEKPNI
TLTDACFTNGKAKGNAVYIIDAKTGQRLWWTSDTGSNTDNANMKHSIVSRISTLDRDADGLVDHLYFGDLGGQIFRVDLN
NNQTKTNSTYSSFGVRVVRLANLATNDSTYDGTNDYTGGNAPRFYEPPTVTIHDYGIHTFITVGIASGDRSTPLDVYPLT
GREGMTPASALSGRPVNNVYGIIDRDFVKKNLMSLTDNQLETKDITRTGLRKNPQILRTGETRVAQIFFPTTGVGKGGWY
RSLSSTSDGTEKANNSFRIKGGLKAFEEPMAITGNLIIPVYDPQGTGIVAADPCLPRVVGETDRQTYCLPFGACLNPDGT
INSNKENPSGFQTKTGSNCPAGVSECNTNVIGAGIRNITFVPKRDEPPVTNSCGKLQLSGNENGTGEWQCTSHLLPVRWY
ERYR

Nucleotide


Download         Length: 3855 bp        

>NTDB_id=974230 ACK8DE_RS17700 WP_023896142.1 3657042..3660896(-) (pilY1) [Acinetobacter baumannii strain MRSN15116]
ATGAAACTAAAATTGAAGAATTTTAAGCCAAATAATTTATGGTATGCCGTCTATTCAAGCTCAATGATATTCACATGGCT
GATGACAAGCTCTGTGGTGCAAGCAAGTGATTTACAAATTTATGCCTCACCTACAGCAGGTAAAAAAACAATTGTGATGA
TGTTGGATACATCAGGAAGTATGACCAACAATAGTTATGGTGAAAACCGCTTAGCCATGCTTAAAAATGGTATGAATGCT
TTTTTGGCTAGCAATAACCCTGTCTTAAATGATACGCGAGTAGGTTTAGGAAATTTCTCGGCAAATGGTGACAGCCGAAG
TGGACAAATTTTAGTGGCTGCTGCTCCTTTAGGTGATGCAAGTACTTTAAATACGGTAGGCTCACAACGTTATAAATTAA
AACAAGCTGTTGCTAATTTAACTGCGGGAGGCTCAACACCTTCAGCCCACGCTTATGCTGAAGCAGCTGCTTACTTAATG
GGGACAACCACATATTCAGAGACGAATTATGCTATTCGTAAAGATAGTTATATCAAACGTGTAAGAAGATCTGATAATAG
AACTGAATATTCATATTGTACTAATTACCGTGATTCGCAAATTGATACAGCCAACCTATGGCAACCTTGTCGTTCAAATA
GTTATTGGAGTAGCTGGTCAACAAATAATCCAGGTGTGGATAATGCTACTGCTTATGATACTTCATCTGACTGGACTTAT
TACTATACTTATTACTACACCACTTTTAATTATGCTGTAGCAAATGCAGATAGCGGTATTCCAAAATCTAAATCAAACGA
TACAGCTAGCAATCCGAATATTGTTGTAGACCGAAATGCTACTAACTCCAATGCAGTGTATCAGTCACCTTTACCTGCAG
TAGCGAATCGTCAGAGTTGTGATGGACAAGGTATTTACTTCTTATCGGATGGCGAACCGAACAATACAACAAATACACGT
TCGGCAAGTGTTATGTCGACTGCTTTAGGTAGTACTTTTGGAGCTGATTTTAATTGTTCTGGAGGTTTATCTAACACTAC
GGCAGACTCTGGTTGGGCTTGTATGGGGGAGTTTGCAAAAAGATTATTTGATAAAACAAAGAATCCTGCCGGAGTTTCTA
TTCAAACAGCATTTGTCGGTTTTGGTAGTGACTTTTCTAGTTTGAGTTCTTCTGATGTAAAAAATGCCTGCCGTTTAAGT
TCGAGAACCCAGTCTGACCGAAAAGGAGATGATGCGTGTTCACCGAATCAATCTACAAATGCGGTAGCTGCACCAGGTTA
TGGAAATGGTGGTTTTTTCCCTACTCAGAGTTCACAAGGTGTAACAGACAGCGTTATCGCATTTATTAATAATTTAGATA
AAGTTCCTTTAGAACCCTTAACCACAGGTGCTATCTCAGTTCCATATGATGCATTGAATCCCAAAAATTTACAGGAATAT
GGTTATTTACGAGCTTTTGAGCCAAATCCAGCTAACACGTACTTAACATGGCGTGGAAATTTAAAGAAATATCATGTGGT
TTTATCTGGAGCGAATGCAGGTGCTTTTGAGGCCAATTCTGGCGGATTAGTCTACAATGCAAGTGGGGCTTTTAGGACTG
GAACAAAGGATTATTGGAATAGCTCTACTTATACTGACGGAGGCAAAGTCTTTTTAGGTGGTTCATATGCGAATGTGCCT
TTACCCATTGCTGGACAACCTGAAACACGTGATGCAGAAGGAAATATCACAAAATATTATTACGCAGTACAAAGTAAAAT
CCGCAACTTATTTACTGATGTTTCCGCTGTTGCAGCAGATGGTAGCTTAACGAAAATTTCAACTTCTGGAACTAATTTGT
TAAAAATTCCAGCCGCTCCACCAGAAGGAACTAATCCTTTTGATACGGTGGCTAATACAGCAAGTTATGTTTTAGGTAAA
TTTGATCCATCAACTGGACAAAATATTTTAAAAGCTTTTCCTATAAGCTTGAAATTAAAAATATTAAATTATTTAGGTTA
TTCAACAGATATTAATGCAACAACTCTGCCTTCATCTTTGGTTACATCGAATGAACCTTATTTATCGATGGGGGGAAGTA
TTCACTCTTTACCGGTTCAACTAACCTACAATGGAACACTAGATGATAATGGGAATTTAACATCTGCCCGAGAACAATCC
ATTCTCTATGGAACCATGGAAGGCGGGTTACATATTGTGGATGCTTCATCTGGTATTGAGCAAATGGTCTTTGTTCCTGC
AGATATTTTAAATGATTCAGTTGCTTCCAAAGCTTTAGTTGTTGGTCAAAGTGATGCTTCAGCTCCCGCTCATGGTATGG
ACGGAGCTTGGGTATCTGATCCAGCCTATAATATTACTACAGTGGGAAGTGGTAGCTCGGCAGTATCAAAAGTAACTGCA
AAGCAAATGAATATTTATGGCGGCATGCGTATGGGAGGAAGTAGCTACTACGGACTAGATGTATTAAGCCCTACTTCACC
GAAACTGCTTTTTAGAATAGGGGCAGACCAGACTGACTATAGCCGTATGGGTCAAAGCTGGTCTAAACCCGTACTCGCGA
ACATCCGTTATAACGGTTCTATTAGACGTGTCCTGATTGTTGGCGGTGGTTATGATCAGTGTTATGAAAAACCAAATATT
ACGTTGACTGACGCTTGCTTTACCAATGGAAAAGCAAAAGGAAATGCTGTCTATATTATTGATGCAAAAACTGGTCAGCG
TTTGTGGTGGACAAGTGATACAGGTTCTAATACTGATAACGCCAATATGAAGCATAGTATTGTTAGCCGTATTAGTACTT
TAGACCGTGATGCTGATGGCTTAGTCGACCATCTATATTTTGGAGATTTAGGCGGACAAATTTTTCGCGTGGACCTTAAT
AATAATCAGACAAAAACCAATTCGACCTATAGCAGTTTTGGTGTCAGAGTTGTGCGTTTAGCGAATTTAGCAACAAATGA
TTCAACTTATGATGGCACAAATGATTATACAGGTGGGAATGCTCCTCGTTTTTATGAGCCTCCAACAGTAACGATCCACG
ATTATGGAATTCACACTTTTATTACAGTAGGAATCGCATCTGGAGACCGTAGTACACCGTTAGATGTTTACCCACTCACA
GGTCGTGAAGGTATGACGCCTGCAAGTGCATTAAGTGGACGTCCTGTAAATAATGTATATGGAATTATTGATAGAGACTT
TGTTAAAAAGAACTTAATGTCTTTAACTGATAATCAGCTTGAAACAAAAGATATTACACGGACAGGCTTAAGAAAAAATC
CACAAATTCTAAGAACAGGTGAAACAAGAGTAGCTCAAATTTTCTTCCCAACTACAGGAGTAGGTAAAGGTGGTTGGTAT
CGCTCGCTTTCTAGTACGAGCGATGGTACAGAAAAAGCTAATAATAGTTTTCGTATTAAAGGAGGACTAAAAGCTTTTGA
GGAACCAATGGCAATTACGGGTAATTTAATTATTCCAGTTTATGACCCTCAAGGAACGGGAATTGTTGCGGCAGATCCTT
GTCTACCTCGTGTTGTGGGAGAAACAGACCGACAAACTTATTGTTTACCATTTGGTGCTTGTCTTAATCCGGATGGAACC
ATTAATTCAAATAAAGAAAACCCGAGCGGGTTTCAAACAAAAACTGGTAGTAACTGTCCAGCTGGAGTCTCTGAATGTAA
TACAAATGTCATTGGTGCAGGTATTCGTAATATCACATTTGTTCCAAAAAGAGATGAACCTCCTGTGACTAATAGTTGTG
GGAAATTACAGCTGTCTGGAAACGAGAACGGAACAGGTGAGTGGCAATGTACAAGTCATTTACTTCCTGTGCGCTGGTAT
GAGCGCTATCGCTAG

Domains

Predicted by InterProScan.

Neisseria PilC beta-propeller domain (T4P_PilY1)

(715-964)

von Willebrand factor type A domain (VWA_2)

(51-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilY1 Acinetobacter baumannii D1279779

88.527

100

0.889