Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AABD49_RS01605 Genome accession   NZ_CP150909
Coordinates   337556..338572 (+) Length   338 a.a.
NCBI ID   WP_031821574.1    Uniprot ID   A0AA47L644
Organism   Vibrio parahaemolyticus strain vp-HL-202008     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 332556..343572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABD49_RS01590 (AABD49_01590) - 332980..333708 (-) 729 WP_015297397.1 redoxin family protein -
  AABD49_RS01595 (AABD49_01595) oxyR 333879..334787 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  AABD49_RS01600 (AABD49_01600) - 334843..337413 (-) 2571 WP_069535787.1 PBP1A family penicillin-binding protein -
  AABD49_RS01605 (AABD49_01605) pilM 337556..338572 (+) 1017 WP_031821574.1 type IV pilus assembly protein PilM Machinery gene
  AABD49_RS01610 (AABD49_01610) pilN 338556..339134 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  AABD49_RS01615 (AABD49_01615) pilO 339127..339720 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  AABD49_RS01620 (AABD49_01620) pilP 339710..340225 (+) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  AABD49_RS01625 (AABD49_01625) pilQ 340257..342005 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  AABD49_RS01630 (AABD49_01630) aroK 342194..342712 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37720.16 Da        Isoelectric Point: 6.6631

>NTDB_id=973670 AABD49_RS01605 WP_031821574.1 337556..338572(+) (pilM) [Vibrio parahaemolyticus strain vp-HL-202008]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKDLPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWNLAAERFPDKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=973670 AABD49_RS01605 WP_031821574.1 337556..338572(+) (pilM) [Vibrio parahaemolyticus strain vp-HL-202008]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGATCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCAGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGTCTAGGTCATATCTGGAATCTTGCCGCAGAGCGTTTTCCTGATAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGAACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATTAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.32

100

0.843
  pilM Vibrio cholerae strain A1552

49.263

100

0.494

Multiple sequence alignment