Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WN982_RS18185 Genome accession   NZ_CP150900
Coordinates   4009980..4011497 (+) Length   505 a.a.
NCBI ID   WP_341313299.1    Uniprot ID   A0AAU6QKZ6
Organism   Paraburkholderia sp. IMGN_8 strain SJ_3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4004980..4016497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WN982_RS18160 (WN982_18160) kdsD 4006169..4007152 (+) 984 WP_341313294.1 arabinose 5-phosphate isomerase KdsD -
  WN982_RS18165 (WN982_18165) - 4007152..4007712 (+) 561 WP_341313295.1 HAD family hydrolase -
  WN982_RS18170 (WN982_18170) lptC 4007709..4008317 (+) 609 WP_341313296.1 LPS export ABC transporter periplasmic protein LptC -
  WN982_RS18175 (WN982_18175) lptA 4008338..4009009 (+) 672 WP_341313297.1 lipopolysaccharide transport periplasmic protein LptA -
  WN982_RS18180 (WN982_18180) lptB 4009006..4009791 (+) 786 WP_341313298.1 LPS export ABC transporter ATP-binding protein -
  WN982_RS18185 (WN982_18185) rpoN/rpoN1 4009980..4011497 (+) 1518 WP_341313299.1 RNA polymerase factor sigma-54 Machinery gene
  WN982_RS18190 (WN982_18190) raiA 4011667..4012026 (+) 360 WP_115099572.1 ribosome-associated translation inhibitor RaiA -
  WN982_RS18195 (WN982_18195) - 4012340..4012855 (+) 516 WP_341313300.1 PTS sugar transporter subunit IIA -
  WN982_RS18200 (WN982_18200) hprK 4012982..4013950 (+) 969 WP_007179412.1 HPr(Ser) kinase/phosphatase -
  WN982_RS18205 (WN982_18205) rapZ 4014050..4014943 (+) 894 WP_341313301.1 RNase adapter RapZ -
  WN982_RS18210 (WN982_18210) - 4014983..4015618 (+) 636 WP_341313302.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55501.60 Da        Isoelectric Point: 5.0680

>NTDB_id=973620 WN982_RS18185 WP_341313299.1 4009980..4011497(+) (rpoN/rpoN1) [Paraburkholderia sp. IMGN_8 strain SJ_3]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAISQNPLLENEDDWIASPLRVAADGSLIAQAPNSSAPPDQM
GGNTSSSSTSSSERTENGEPQGVDEYNSLAGDSNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVT
QAGQRDRALITFLIESLDDDGYLTATLDEVLADMPEELEVDLDELNAALALLHSFDPAGVGARSASECLKLQLLRLDGSP
TRTLALDIVAHHLELLAARDFTRLRKHLKASDDDLRDAHLLIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENPKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=973620 WN982_RS18185 WP_341313299.1 4009980..4011497(+) (rpoN/rpoN1) [Paraburkholderia sp. IMGN_8 strain SJ_3]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGCTGACCCCGCAACTGCAGCAGTCCATCCGGCTGCTTCA
GCTGTCTACGCTCGAACTGCAGCAGGAAGTCGCAATGGCGATCTCGCAGAATCCGCTCCTCGAGAACGAGGATGACTGGA
TCGCGAGTCCCCTGCGCGTGGCGGCCGACGGCTCACTGATCGCCCAGGCGCCCAACTCCTCCGCGCCTCCCGACCAGATG
GGCGGCAATACGTCGTCCTCGTCCACCAGCAGCAGCGAGCGCACCGAGAACGGCGAGCCGCAGGGCGTCGACGAATATAA
CAGCCTTGCGGGAGACAGCAACGGCGACGCGTCGCAATGGAATCTCGACGACTACGGCCGCTCCGGCAACGCGTCGGACG
ACGACGATCTGCCGCCGCTGCAAATCCACGAATCGAGCACCTCGCTGCGCGATCACCTGATGGCGCAATTGCGCGTTACC
CAGGCGGGCCAGCGCGACCGCGCGCTGATCACCTTCCTGATCGAATCGCTCGACGACGACGGCTACCTCACCGCCACGCT
GGACGAAGTGCTGGCCGACATGCCTGAAGAGCTCGAAGTCGACCTCGACGAATTGAACGCCGCGCTCGCGCTGCTGCATA
GTTTCGACCCGGCAGGTGTCGGCGCGCGCTCCGCGTCCGAATGTCTCAAGCTGCAACTGCTGCGGCTCGACGGCTCGCCT
ACCCGCACGCTCGCGCTCGACATCGTTGCCCATCATCTGGAACTGCTCGCGGCGCGCGATTTCACACGCCTGCGCAAACA
TCTGAAGGCCAGCGACGACGACCTGCGCGATGCGCATCTGTTGATACGCTCGCTCGAGCCGTTTCCCGGCGCGGCTTACG
GCAAGGCGGAAGCGGACTATGTCGTGCCGGACATCATGGTGCGCAAAACGGCACAGGGCTGGCAGGCAGAACTGAACCCG
GAAGTCGTGCCGAAGCTGCGGATCAACCATCTTTACGCGAATATTCTGCGCAATAACCGGGGCGACCCGGGCAGCGGTTC
GTTGCGCCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGGTTCGAGACGATCCTCAGGGTCGCGC
AAGCTATTGTGGAGCGTCAAAAGAGCTTTTTTGTGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATTGCT
GATACGCTGGGCCTACACGAGTCGACTGTCTCGCGTGTGACAACCGGTAAATACATGCTGACCCCATTCGGGACGCTTGA
ATTTAAGTACTTCTTCGGATCACACGTCTCAACCGACACGGGCGGCGCGGCCTCCTCCACGGCGATTCGCGCGCTCATCA
AGCAACTGATAGGAGCGGAAAACCCGAAATCTCCTCTTTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCACGGCGTACCGTTGCGAAGTACCGCGAAGCGCTCAAGATTCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-332)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.992

100

0.679

Multiple sequence alignment