Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   WF295_RS13620 Genome accession   NZ_CP150885
Coordinates   2723161..2723454 (-) Length   97 a.a.
NCBI ID   WP_020452176.1    Uniprot ID   A0A6N2FHJ1
Organism   Bacillus paralicheniformis strain Baplich1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2718161..2728454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WF295_RS13575 (WF295_13575) - 2718538..2719332 (-) 795 WP_020452167.1 TasA family protein -
  WF295_RS13580 (WF295_13580) - 2719405..2719989 (-) 585 WP_020452168.1 signal peptidase I -
  WF295_RS13585 (WF295_13585) tapA 2719986..2720714 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  WF295_RS13590 (WF295_13590) - 2720992..2721312 (+) 321 WP_020452170.1 YqzG/YhdC family protein -
  WF295_RS13595 (WF295_13595) - 2721342..2721524 (-) 183 WP_020452171.1 YqzE family protein -
  WF295_RS13600 (WF295_13600) comGG 2721613..2721978 (-) 366 WP_020452172.1 competence type IV pilus minor pilin ComGG -
  WF295_RS13605 (WF295_13605) comGF 2721990..2722478 (-) 489 WP_236613657.1 competence type IV pilus minor pilin ComGF -
  WF295_RS13610 (WF295_13610) comGE 2722387..2722734 (-) 348 WP_020452174.1 competence type IV pilus minor pilin ComGE -
  WF295_RS13615 (WF295_13615) comGD 2722718..2723155 (-) 438 WP_020452175.1 competence type IV pilus minor pilin ComGD -
  WF295_RS13620 (WF295_13620) comGC 2723161..2723454 (-) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  WF295_RS13625 (WF295_13625) comGB 2723468..2724502 (-) 1035 WP_041817253.1 competence type IV pilus assembly protein ComGB Machinery gene
  WF295_RS13630 (WF295_13630) comGA 2724492..2725556 (-) 1065 WP_020452178.1 competence type IV pilus ATPase ComGA Machinery gene
  WF295_RS13635 (WF295_13635) - 2725714..2726553 (-) 840 WP_020452179.1 STAS domain-containing protein -
  WF295_RS13645 (WF295_13645) - 2726795..2727172 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  WF295_RS13650 (WF295_13650) - 2727383..2727628 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  WF295_RS13655 (WF295_13655) - 2727664..2728302 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -

Sequence


Protein


Download         Length: 97 a.a.        Molecular weight: 10670.54 Da        Isoelectric Point: 9.1200

>NTDB_id=973420 WF295_RS13620 WP_020452176.1 2723161..2723454(-) (comGC) [Bacillus paralicheniformis strain Baplich1]
MNEKGFTLIEMLVVMLVISILLLITIPNVTKHNQSIQKKGCEGLINMVQAQITAYQMDHDGKTPSRADLESEGYVKKNLK
CPNGKAIKISNGKAQAD

Nucleotide


Download         Length: 294 bp        

>NTDB_id=973420 WF295_RS13620 WP_020452176.1 2723161..2723454(-) (comGC) [Bacillus paralicheniformis strain Baplich1]
GTGAATGAAAAGGGATTTACACTGATAGAAATGTTAGTCGTCATGCTCGTCATCTCCATCCTGCTGTTGATCACCATACC
GAATGTGACCAAGCATAATCAAAGTATTCAAAAGAAAGGATGCGAAGGGTTAATTAATATGGTTCAAGCGCAAATCACGG
CTTATCAAATGGATCATGACGGGAAAACGCCGAGTAGGGCGGACCTTGAATCTGAAGGTTACGTGAAGAAGAACTTAAAG
TGCCCGAATGGGAAAGCAATTAAAATTTCAAACGGCAAAGCACAGGCTGATTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6N2FHJ1

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

71.134

100

0.711

  comGC Staphylococcus aureus MW2

45.263

97.938

0.443

  comGC Staphylococcus aureus N315

45.263

97.938

0.443


Multiple sequence alignment