Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AACK07_RS01860 Genome accession   NZ_AP029012
Coordinates   414749..416305 (-) Length   518 a.a.
NCBI ID   WP_028853770.1    Uniprot ID   A0ABX7ZQ67
Organism   Ralstonia pseudosolanacearum strain MAFF 301069     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 409749..421305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACK07_RS01840 - 410076..410228 (-) 153 WP_240438048.1 hypothetical protein -
  AACK07_RS01845 (MAFF301069_03650) - 410256..411545 (-) 1290 WP_118871965.1 hypothetical protein -
  AACK07_RS01850 (MAFF301069_03660) - 411915..413198 (-) 1284 Protein_368 hypothetical protein -
  AACK07_RS01855 (MAFF301069_03670) gspF 413536..414747 (-) 1212 WP_028861094.1 type II secretion system inner membrane protein GspF -
  AACK07_RS01860 (MAFF301069_03680) pilF 414749..416305 (-) 1557 WP_028853770.1 type II secretion system ATPase GspE Machinery gene
  AACK07_RS01865 (MAFF301069_03690) gspD 416340..418760 (-) 2421 WP_118871967.1 type II secretion system secretin GspD -
  AACK07_RS01870 (MAFF301069_03700) - 418794..419651 (-) 858 WP_118871968.1 type II secretion system protein N -
  AACK07_RS01875 (MAFF301069_03710) gspM 419655..420233 (-) 579 WP_011003003.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 518 a.a.        Molecular weight: 55861.86 Da        Isoelectric Point: 5.5004

>NTDB_id=97298 AACK07_RS01860 WP_028853770.1 414749..416305(-) (pilF) [Ralstonia pseudosolanacearum strain MAFF 301069]
MATQASSTDARTLEPPSPSAVRLVPYVFARDARLLVARQDVDSLEVWVCPETSRAALAELARVFGALRLVSLDAAALSAA
TQTAYNAQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFENASVVRFR
VDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDL
GKLGMGAGTLGRFDRLINQPHGIVLVTGPTGSGKTTTLYAALSRLDASSTNIMTVEDPIEYDLDGIGQTQVNAKIDMTFA
KALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLSSSLLGVLAQRLVRRL
CVHCRREEVLELTPTEIEAVAGTPPADGTAAAVPTRRSVWHHVGCEHCGQSGYQGRTGVYELLTVTPEIQTMIHRQAPES
EIKALAIGQGMHTMRADAQRWLDAGATSLEEVLRVTRD

Nucleotide


Download         Length: 1557 bp        

>NTDB_id=97298 AACK07_RS01860 WP_028853770.1 414749..416305(-) (pilF) [Ralstonia pseudosolanacearum strain MAFF 301069]
ATGGCAACGCAAGCTTCTTCCACTGACGCGCGCACGCTCGAGCCGCCGTCGCCCTCGGCGGTGCGGCTGGTGCCGTATGT
GTTCGCGCGCGACGCCAGGCTGCTGGTGGCGCGGCAGGATGTCGACTCGCTCGAGGTGTGGGTCTGCCCCGAGACCTCGC
GCGCCGCGCTGGCCGAGCTGGCCCGCGTGTTCGGCGCGCTGCGCCTGGTGAGCCTGGACGCCGCCGCGCTCTCCGCCGCC
ACGCAGACGGCCTACAACGCCCAGGACGGCAGCGCGGCCCAGGTGGTCGGCGAGGTCGAGGGCGAGGTGGACCTGTCGCG
CCTGATGCAGGACATCCCGGCCGTGGAAGACCTGCTCGAATCGGAAGACGACGCGCCGATCATCCGCATGATCAACGCGC
TGCTCACGCAGGCCGCACGCGAGGGCGCCTCGGACATCCACATCGAGCCGTTCGAGAACGCCTCGGTGGTGCGCTTCCGC
GTGGACGGCACGCTGCGCGACGTGGTGCGCCCGAAGAAGGCGCTGCACGGCGCGCTGATCTCGCGCATCAAGATCATGGC
GCAGCTCGACATCGCCGAGAAACGCCTGCCGCAGGACGGCCGCATCACGCTGCGCGTGGGCGGGCGACCGGTGGACGTGC
GTGTCTCCACCCTGCCGACCGGCCACGGCGAGCGCGCGGTGCTGCGCCTGCTCGACAAGGAAGCCGGCCGGCTCGACCTC
GGCAAGCTCGGCATGGGCGCCGGCACGCTGGGGCGCTTCGACCGCCTGATCAACCAGCCGCACGGCATCGTGCTCGTCAC
CGGCCCGACCGGCTCGGGCAAGACCACCACGCTGTATGCCGCGCTGTCGCGGCTCGATGCCAGCAGCACCAACATCATGA
CGGTGGAGGACCCGATCGAGTACGACCTGGACGGTATCGGCCAGACCCAGGTCAACGCGAAGATCGACATGACCTTCGCC
AAGGCCCTGCGCGCCATCCTGCGACAGGACCCGGACGTGGTGATGATCGGGGAGATCCGCGACCTGGAGACCGCGCAGAT
CGCGGTGCAGGCCTCGCTGACCGGCCACCTGGTGCTGGCGACGCTGCACACCAACGATTCGGCCTCGGCGGTCACGCGGC
TGATCGACATGGGGATCGAGCCGTTCCTGCTGTCGTCGTCGCTGCTGGGCGTGCTGGCGCAGCGGCTGGTGCGGCGCCTG
TGCGTGCACTGCCGCCGCGAGGAAGTGCTGGAGCTCACCCCCACCGAGATCGAGGCCGTGGCCGGCACGCCGCCCGCCGA
CGGCACCGCCGCCGCCGTGCCGACGCGCCGCTCGGTGTGGCACCACGTCGGCTGCGAACACTGCGGCCAATCGGGCTACC
AGGGCCGGACCGGCGTGTACGAGCTGCTGACGGTGACGCCCGAGATCCAGACCATGATCCACCGCCAGGCGCCCGAGTCC
GAGATCAAGGCGCTCGCCATCGGCCAGGGCATGCATACCATGCGCGCGGATGCGCAGCGGTGGCTCGACGCGGGCGCGAC
GTCGCTGGAAGAGGTGCTGCGCGTGACGCGCGACTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(133-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

42.744

97.104

0.415
  pilB Vibrio cholerae strain A1552

45.657

86.68

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.689

99.228

0.394
  pilB Vibrio campbellii strain DS40M4

44.835

87.838

0.394
  pilB/pilB1 Synechocystis sp. PCC 6803

44.676

83.398

0.373
  pilB Vibrio parahaemolyticus RIMD 2210633

46.324

78.764

0.365

Multiple sequence alignment