Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   WOC53_RS10340 Genome accession   NZ_CP150781
Coordinates   2070613..2071110 (+) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM20820     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2065613..2076110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOC53_RS10300 (WOC53_10295) - 2065657..2066643 (+) 987 WP_000161314.1 ROK family glucokinase -
  WOC53_RS10305 (WOC53_10300) - 2066643..2066972 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  WOC53_RS10310 (WOC53_10305) - 2066969..2067592 (+) 624 WP_001223010.1 MBL fold metallo-hydrolase -
  WOC53_RS10315 (WOC53_10310) comGA 2067644..2068618 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  WOC53_RS10320 (WOC53_10315) comGB 2068590..2069660 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  WOC53_RS10325 (WOC53_10320) comGC 2069674..2069985 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  WOC53_RS10330 (WOC53_10325) comGD 2069963..2070409 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  WOC53_RS10335 (WOC53_10330) comGE 2070396..2070695 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  WOC53_RS10340 (WOC53_10335) comGF 2070613..2071110 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  WOC53_RS10345 (WOC53_10340) - 2071207..2071353 (+) 147 WP_001789879.1 hypothetical protein -
  WOC53_RS10350 (WOC53_10345) - 2071343..2071867 (+) 525 WP_001015121.1 shikimate kinase -
  WOC53_RS10355 (WOC53_10350) gcvT 2072026..2073117 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  WOC53_RS10360 (WOC53_10355) gcvPA 2073137..2074483 (+) 1347 WP_117200971.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  WOC53_RS10365 (WOC53_10360) gcvPB 2074476..2075948 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=972544 WOC53_RS10340 WP_001789864.1 2070613..2071110(+) (comGF) [Staphylococcus aureus strain TUM20820]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=972544 WOC53_RS10340 WP_001789864.1 2070613..2071110(+) (comGF) [Staphylococcus aureus strain TUM20820]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment