Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WJM45_RS16705 Genome accession   NZ_CP150634
Coordinates   3988516..3989418 (-) Length   300 a.a.
NCBI ID   WP_341326194.1    Uniprot ID   -
Organism   Methylotuvimicrobium sp. KM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3983516..3994418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM45_RS16670 (WJM45_16670) - 3983614..3983952 (+) 339 WP_341326187.1 J domain-containing protein -
  WJM45_RS16675 (WJM45_16675) grpE 3983949..3984599 (+) 651 WP_341326188.1 nucleotide exchange factor GrpE -
  WJM45_RS16680 (WJM45_16680) - 3984617..3986371 (+) 1755 WP_341326189.1 Hsp70 family protein -
  WJM45_RS16685 (WJM45_16685) - 3986371..3986793 (+) 423 WP_341326190.1 hypothetical protein -
  WJM45_RS16690 (WJM45_16690) yacG 3986926..3987132 (-) 207 WP_341326191.1 DNA gyrase inhibitor YacG -
  WJM45_RS16695 (WJM45_16695) zapD 3987132..3987902 (-) 771 WP_341326192.1 cell division protein ZapD -
  WJM45_RS16700 (WJM45_16700) coaE 3987906..3988511 (-) 606 WP_341326193.1 dephospho-CoA kinase -
  WJM45_RS16705 (WJM45_16705) pilD 3988516..3989418 (-) 903 WP_341326194.1 A24 family peptidase Machinery gene
  WJM45_RS16710 (WJM45_16710) - 3991517..3991621 (+) 105 WP_341328966.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 33124.22 Da        Isoelectric Point: 6.9380

>NTDB_id=970751 WJM45_RS16705 WP_341326194.1 3988516..3989418(-) (pilD) [Methylotuvimicrobium sp. KM2]
MQGLEIFADSPAALMALTGVIGLMVGSFLNVVIYRLPIMMQKSWRSECIDYLKSTGDAQFVGLDTGRGETDSKFNLVFPL
SRCPHCETPIKPYQNIPVFSYLLLKGRCAQCNAPISKRYPVIEGLTGFFSVLIAWHFGYSVEVLFALILTWSLIALSAID
IDHQLLPDSITLPLLWLGLFLSLFDIFIDTHSSIIGAIAGYLSLWTVYHGFKLLTGKEGMGYGDFKLLAMFGAWLGWQYI
LPIILLSSLVGAVTGIAMVILVKRDKNIPIPFGPYLAAAGWIALLWGDKINQAYLNFAGL

Nucleotide


Download         Length: 903 bp        

>NTDB_id=970751 WJM45_RS16705 WP_341326194.1 3988516..3989418(-) (pilD) [Methylotuvimicrobium sp. KM2]
ATGCAAGGACTAGAAATATTCGCCGATTCGCCGGCTGCTCTAATGGCTTTAACCGGTGTTATCGGTCTCATGGTCGGTAG
TTTCCTTAACGTCGTTATTTACCGTTTGCCGATTATGATGCAAAAAAGTTGGCGCAGTGAATGCATCGATTATTTGAAGT
CCACGGGGGATGCGCAATTCGTGGGTCTTGATACAGGAAGAGGGGAAACCGACTCAAAATTTAACTTAGTGTTTCCATTG
TCGCGCTGTCCGCACTGCGAAACACCGATAAAGCCTTATCAAAATATTCCGGTGTTCAGTTATTTGTTGTTGAAAGGCCG
TTGCGCTCAATGCAATGCGCCGATATCGAAACGCTATCCCGTTATCGAGGGATTAACCGGTTTTTTTTCGGTTTTAATCG
CCTGGCATTTCGGTTATTCGGTAGAGGTTCTATTCGCTCTCATACTCACCTGGTCCTTGATTGCGCTGAGTGCAATCGAT
ATTGACCATCAACTTTTGCCGGATTCGATTACATTACCTCTACTTTGGTTGGGTTTGTTTTTAAGTCTTTTCGATATCTT
CATCGATACTCATTCTTCGATTATCGGTGCTATAGCCGGTTATTTATCACTTTGGACGGTTTATCATGGTTTCAAGCTGT
TGACCGGCAAAGAGGGCATGGGATACGGCGACTTCAAGCTGTTGGCAATGTTTGGGGCTTGGTTGGGTTGGCAGTATATT
TTGCCGATCATTTTGCTGTCATCCTTGGTCGGCGCCGTTACCGGCATCGCGATGGTTATTTTGGTGAAGCGCGACAAGAA
TATTCCAATTCCATTCGGTCCTTACTTGGCCGCGGCCGGGTGGATCGCTTTATTATGGGGCGATAAAATCAATCAGGCTT
ATCTCAATTTTGCCGGACTATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(147-256)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

50.34

98

0.493
  pilD Vibrio campbellii strain DS40M4

48.98

98

0.48
  pilD Acinetobacter baumannii D1279779

49.481

96.333

0.477
  pilD Acinetobacter nosocomialis M2

49.135

96.333

0.473
  pilD Neisseria gonorrhoeae MS11

47.887

94.667

0.453

Multiple sequence alignment