Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AACK08_RS01615 Genome accession   NZ_AP029004
Coordinates   346994..348550 (-) Length   518 a.a.
NCBI ID   WP_071507745.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain MAFF 211519     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 341994..353550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACK08_RS01600 (MAFF211519_03180) - 342828..344114 (-) 1287 WP_020371584.1 hypothetical protein -
  AACK08_RS01605 (MAFF211519_03190) - 344190..345473 (-) 1284 WP_011003008.1 hypothetical protein -
  AACK08_RS01610 (MAFF211519_03200) gspF 345781..346992 (-) 1212 WP_021154964.1 type II secretion system inner membrane protein GspF -
  AACK08_RS01615 (MAFF211519_03210) pilF 346994..348550 (-) 1557 WP_071507745.1 type II secretion system ATPase GspE Machinery gene
  AACK08_RS01620 (MAFF211519_03220) gspD 348585..351005 (-) 2421 WP_071012356.1 type II secretion system secretin GspD -
  AACK08_RS01625 (MAFF211519_03230) - 351039..351890 (-) 852 WP_019718954.1 type II secretion system protein N -
  AACK08_RS01630 (MAFF211519_03240) gspM 351894..352472 (-) 579 WP_028861093.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 518 a.a.        Molecular weight: 55910.93 Da        Isoelectric Point: 5.5138

>NTDB_id=97056 AACK08_RS01615 WP_071507745.1 346994..348550(-) (pilF) [Ralstonia pseudosolanacearum strain MAFF 211519]
MATQASSTDARTLEPPSPSAVRLVPYVFARDARLLVARQDVDSLEVWVCPETSRAALAELARVFGALRLVSLDAAALSAA
TQTAYNAQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFENASVVRFR
VDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDL
GKLGMGAGTLGRFDRLINQPHGIVLVTGPTGSGKTTTLYAALSRLDASSTNIMTVEDPIEYDLDGIGQTQVNAKIDMTFA
KALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAVTRLIDMGIEPFLLSSSLLGVLAQRLVRRL
CVHCRREEVLELTPTEIEAVAGTPPADGTAAAVPTRRSVWHHVGCERCGQSGYQGRTGVYELLTVTPEIQTMIHRQAPES
EIKALAIGQGMHTMRADAQRWLDTGATSLEEVLRVTRD

Nucleotide


Download         Length: 1557 bp        

>NTDB_id=97056 AACK08_RS01615 WP_071507745.1 346994..348550(-) (pilF) [Ralstonia pseudosolanacearum strain MAFF 211519]
ATGGCAACGCAAGCTTCTTCCACTGACGCGCGCACGCTCGAGCCGCCGTCGCCCTCGGCGGTGCGGCTGGTGCCGTATGT
GTTCGCGCGCGACGCCAGGCTGCTGGTGGCGCGGCAGGATGTCGACTCGCTCGAGGTGTGGGTCTGCCCCGAGACCTCGC
GCGCCGCGCTGGCCGAGCTGGCCCGCGTGTTCGGCGCGCTGCGCCTGGTGAGCCTGGACGCCGCCGCGCTCTCCGCCGCC
ACGCAGACGGCCTACAACGCCCAGGACGGCAGCGCGGCCCAGGTGGTCGGCGAGGTCGAGGGCGAGGTGGACCTGTCGCG
CCTGATGCAGGACATCCCGGCCGTGGAAGACCTGCTCGAATCGGAAGACGACGCGCCGATCATCCGCATGATCAACGCGC
TGCTCACGCAGGCCGCACGCGAGGGCGCCTCGGACATCCACATCGAGCCGTTCGAGAACGCCTCGGTGGTGCGCTTCCGC
GTGGACGGCACGCTGCGCGACGTGGTGCGCCCGAAGAAGGCGCTGCACGGCGCGCTGATCTCGCGCATCAAGATCATGGC
GCAGCTCGACATCGCCGAGAAACGCCTGCCGCAGGACGGCCGCATCACGCTGCGCGTGGGCGGGCGACCGGTGGACGTGC
GTGTCTCCACCCTGCCGACCGGCCACGGCGAGCGCGCGGTGCTGCGCCTGCTCGACAAGGAAGCCGGCCGGCTCGACCTC
GGCAAGCTCGGCATGGGCGCCGGCACGCTGGGGCGCTTCGACCGCCTGATCAACCAGCCGCACGGCATCGTGCTCGTCAC
CGGCCCGACCGGCTCGGGCAAGACCACCACGCTGTATGCCGCGCTGTCGCGGCTCGATGCCAGCAGCACCAACATCATGA
CGGTGGAGGACCCGATCGAGTACGACCTGGACGGTATCGGCCAGACCCAGGTCAACGCGAAGATCGACATGACCTTCGCC
AAGGCCCTGCGCGCCATCCTGCGGCAGGACCCGGACGTGGTGATGATCGGCGAGATCCGCGACCTGGAGACCGCGCAGAT
CGCGGTGCAGGCCTCGCTGACCGGCCACCTGGTGCTGGCGACGCTGCACACCAACGATTCGGCCTCGGCGGTCACGCGGC
TGATCGACATGGGGATCGAGCCGTTCCTGCTGTCGTCGTCGCTGCTGGGCGTGCTGGCGCAGCGGCTGGTGCGGCGCCTG
TGCGTGCACTGCCGCCGCGAGGAAGTGCTGGAGCTCACCCCCACCGAGATCGAGGCCGTGGCCGGCACGCCGCCCGCCGA
CGGCACCGCCGCCGCCGTGCCGACGCGCCGCTCGGTGTGGCACCACGTCGGCTGCGAACGCTGCGGCCAATCGGGCTACC
AGGGCCGGACCGGCGTGTACGAGCTGCTGACGGTGACGCCCGAGATCCAGACCATGATCCACCGCCAGGCGCCCGAGTCC
GAGATCAAGGCGCTCGCCATCGGCCAGGGCATGCATACCATGCGCGCGGATGCGCAGCGGTGGCTGGACACGGGCGCGAC
GTCGCTGGAAGAGGTGCTGCGCGTGACGCGCGACTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(133-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

42.942

97.104

0.417
  pilB Vibrio cholerae strain A1552

45.657

86.68

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.883

99.228

0.396
  pilB Vibrio campbellii strain DS40M4

44.835

87.838

0.394
  pilB/pilB1 Synechocystis sp. PCC 6803

44.676

83.398

0.373
  pilB Vibrio parahaemolyticus RIMD 2210633

46.324

78.764

0.365

Multiple sequence alignment