Detailed information NTG0970274

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: ACLF6N_RS09705
• Genome accession: NZ_CP177279
• Coordinates: 1827195..1829771 (+)
• Length: 858 a.a.
• NCBI ID: WP_014479821.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain KFRI-P74
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 1822195..1834771

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLF6N_RS09685 (ACLF6N_09685)	kbl	1822967..1824145 (+)	1179	WP_014479819.1	glycine C-acetyltransferase	-
ACLF6N_RS09690 (ACLF6N_09690)	miaB	1824293..1825822 (+)	1530	WP_014479820.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
ACLF6N_RS09695 (ACLF6N_09695)	ymcA	1825824..1826255 (+)	432	WP_003231834.1	regulatory iron-sulfur-containing complex subunit RicA	Regulator
ACLF6N_RS09700 (ACLF6N_09700)	cotE	1826517..1827062 (+)	546	WP_003231833.1	outer spore coat protein CotE	-
ACLF6N_RS09705 (ACLF6N_09705)	hexA	1827195..1829771 (+)	2577	WP_014479821.1	DNA mismatch repair protein MutS	Machinery gene
ACLF6N_RS09710 (ACLF6N_09710)	hexB	1829787..1831670 (+)	1884	WP_041516431.1	DNA mismatch repair endonuclease MutL	Machinery gene
ACLF6N_RS09715 (ACLF6N_09715)	-	1832043..1832189 (+)	147	WP_014479823.1	XtrA/YqaO family protein	-
ACLF6N_RS09720 (ACLF6N_09720)	-	1832232..1832483 (+)	252	Protein_1859	VWA domain-containing protein	-
ACLF6N_RS09725 (ACLF6N_09725)	-	1832780..1833028 (+)	249	WP_031600520.1	hypothetical protein	-
ACLF6N_RS09730 (ACLF6N_09730)	-	1833079..1833441 (-)	363	WP_033881685.1	Ig-like domain-containing protein	-
ACLF6N_RS09735 (ACLF6N_09735)	-	1833714..1834463 (+)	750	WP_014479826.1	hypothetical protein	-

Sequence

Protein

• Length: 858 a.a.
• Molecular weight: 97672.80 Da
• Isoelectric Point: 5.3012

>NTDB_id=970274 ACLF6N_RS09705 WP_014479821.1 1827195..1829771(+) (hexA) [Bacillus subtilis strain KFRI-P74]
MAGYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGAAEKIPMCGVPYHSASAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSACSNRYGLALSDLTTGENLAVLIERLEDV
ISEIYSVGAREIVVSGGLDADTVAQLRERCGATISIEDGETDEHVTIIEHLNNEDITKTFLRLYTYLKRTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKNKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRVNQIEERQEMVETLMSHFF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPGIKQLVASLAHDKAKERAKRIDPCGDVLELLEEALYE
NPPLSVKEGNLIKDGYNQKLDEYRDASRNGKDWIARLEQQEREYTGIRSLKVGFNKVFGYYIEVTKANLHLLEEGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFTELREKVKQYIPRLQQLAKQMSELDALQCFATISENRHYTKPEF
SKDEVEVIEGRHPVVEKVMDSQEYVPNNCMMGDNRQMLLITGPNMSGKSTYMRQIALISIMAQIGCFVPAKKAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKNSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPGDLIARAQDILKELEHSGNKPEVPVQKPQV
KEEPAQLSFFDEAEKPAETPKLSKKEKQVIDAFKSLNILDMTPLEAMNEMYKLQKKLH

Nucleotide

• Length: 2577 bp

>NTDB_id=970274 ACLF6N_RS09705 WP_014479821.1 1827195..1829771(+) (hexA) [Bacillus subtilis strain KFRI-P74]
ATGGCCGGTTATACGCCTATGATACAGCAATATTTAAAAATAAAGGCAGAGCACCAGGATGCCTTTTTATTTTTTCGCCT
GGGTGATTTTTACGAAATGTTTTTTGAGGACGCCAAAAAAGCGTCACAAGAGCTGGAAATTACGTTAACGAGCAGAGATG
GCGGTGCGGCTGAAAAAATACCGATGTGCGGTGTGCCGTATCATTCTGCTTCCGCGTATATCGAACAGCTTATTAAAAAA
GGATACAAAGTGGCGATCTGTGAACAGACGGAAGATCCGAAAGCCGCAAAGGGCGTTGTGAAAAGAGAAGTGGTTCAGCT
GATTACGCCCGGAACTGTAATGGACGGCAAAGGCATCCATGAATCGGAAAATAACTTTATCGCATCTGTTTCAGCCTGCT
CGAACAGATACGGACTTGCGCTGTCTGATTTAACAACGGGAGAAAATTTGGCTGTTTTGATTGAACGGCTTGAAGACGTC
ATATCAGAAATTTATTCAGTCGGCGCACGGGAAATCGTGGTTTCAGGAGGCTTGGATGCCGATACAGTGGCACAGCTGAG
AGAGCGGTGCGGTGCAACAATTTCAATTGAAGACGGAGAAACAGACGAACACGTAACGATCATTGAGCACTTAAATAATG
AAGATATAACAAAAACATTTCTGCGCTTATATACGTATTTGAAAAGAACCCAAAAGCGCAGCCTTGATCATCTTCAGCCC
GTGCAGGTGTATGAGCTTGAGGAAGCGATGAAAATTGACTTGTACTCAAAGCGTAATCTGGAGCTGACCGAAACGATTCG
TTCGAAAAATAAAAAAGGCTCCCTTTTGTGGCTGCTGGATGAAACAAAAACGGCAATGGGAGGCCGGCTGCTTAAACAGT
GGATTGACCGGCCGCTTATCAGAGTCAATCAAATCGAAGAGCGCCAAGAAATGGTGGAAACATTGATGTCCCATTTCTTT
GAACGGGAAGATCTGCGTGAACGTTTAAAAGAAGTGTATGACTTAGAACGCCTTGCCGGCCGCGTTGCATTCGGAAATGT
CAATGCCCGGGATTTGATTCAGCTGAAGGAATCGTTAAAGCAAGTTCCTGGCATCAAACAGCTGGTTGCTTCATTGGCTC
ATGACAAGGCCAAGGAACGTGCAAAGCGCATTGACCCTTGCGGGGATGTACTTGAACTGCTGGAAGAAGCGCTGTACGAA
AACCCTCCTTTATCGGTAAAAGAAGGGAACCTGATTAAAGATGGTTATAATCAAAAGCTTGATGAATATCGTGATGCAAG
CAGAAACGGAAAAGACTGGATTGCCCGCCTGGAACAGCAGGAGCGGGAATACACAGGCATTCGCTCTTTAAAGGTCGGCT
TTAATAAAGTTTTCGGTTATTACATTGAAGTGACTAAAGCAAACTTGCATTTGCTTGAGGAAGGGCGTTATGAGCGGAAG
CAGACGTTAACCAATGCCGAGCGCTACATTACACCTGAATTAAAAGAAAAAGAAGCGCTCATTTTAGAGGCGGAAAATAA
CATCTGTGAGCTGGAGTACGAGCTGTTTACCGAGCTGCGCGAGAAAGTGAAGCAGTATATTCCGCGGCTGCAGCAGCTTG
CAAAACAGATGAGCGAGCTGGACGCACTGCAATGCTTTGCGACAATCAGTGAAAATCGTCACTACACGAAACCAGAGTTC
TCTAAAGATGAAGTCGAAGTGATTGAAGGCAGACACCCGGTTGTTGAAAAAGTCATGGACAGCCAGGAATATGTCCCGAA
TAACTGTATGATGGGCGATAACAGACAAATGCTTCTCATTACAGGTCCAAACATGTCAGGGAAAAGCACGTATATGAGAC
AAATCGCGCTCATTTCCATTATGGCGCAAATCGGCTGCTTTGTACCCGCGAAAAAAGCGGTGCTTCCGATTTTCGATCAA
ATTTTTACGCGAATCGGCGCTGCAGATGATTTGATTTCCGGACAAAGTACATTTATGGTAGAAATGCTTGAAGCGAAAAA
TGCGATTGTCAACGCGACGAAAAACAGCCTCATTCTGTTTGATGAAATCGGGCGGGGAACGTCCACTTATGACGGCATGG
CGCTGGCACAGGCGATTATTGAATATGTTCACGATCACATCGGCGCCAAGACGCTGTTCAGCACACACTATCACGAGCTG
ACCGTTCTTGAGGACAAGCTACCCCAGCTGAAAAACGTTCATGTTCGCGCTGAAGAATATAACGGAACGGTTGTCTTTCT
TCATCAAATTAAAGAAGGTGCCGCTGATAAAAGCTATGGTATCCATGTAGCTCAGCTTGCCGAACTGCCGGGAGATCTGA
TTGCGCGCGCTCAAGATATTTTAAAAGAGCTTGAGCATTCAGGAAACAAACCGGAAGTGCCGGTGCAGAAACCTCAGGTG
AAAGAAGAGCCGGCACAGCTGTCCTTTTTTGACGAAGCGGAAAAGCCGGCTGAAACACCAAAGCTTTCAAAAAAAGAAAA
GCAAGTGATCGATGCTTTCAAATCACTTAATATACTGGATATGACACCGCTTGAAGCGATGAATGAAATGTACAAGCTGC
AAAAGAAATTACATTAA

Domains

Predicted by InterProScan.

• MutS domain II (MutS_II) (125-239)

• MutS family domain IV (MutS_IV) (415-506)

• MutS domain I (MutS_I) (5-116)

• MutS domain III (MutS_III) (258-547)

• MutS domain V (MutS_V) (598-785)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	49.474	99.65	0.493
mutS	Pseudomonas stutzeri strain ATCC 17587	38.673	100	0.394