Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   MHB62_RS12180 Genome accession   NZ_CP150278
Coordinates   2385126..2385509 (-) Length   127 a.a.
NCBI ID   WP_032722118.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0255     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2380126..2390509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB62_RS12140 (MHB62_12140) sinI 2381059..2381232 (+) 174 WP_003230187.1 anti-repressor SinI Regulator
  MHB62_RS12145 (MHB62_12145) sinR 2381266..2381601 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  MHB62_RS12150 (MHB62_12150) tasA 2381694..2382479 (-) 786 WP_014664586.1 biofilm matrix protein TasA -
  MHB62_RS12155 (MHB62_12155) sipW 2382543..2383115 (-) 573 WP_072692741.1 signal peptidase I SipW -
  MHB62_RS12160 (MHB62_12160) tapA 2383099..2383860 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  MHB62_RS12165 (MHB62_12165) - 2384132..2384458 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  MHB62_RS12170 (MHB62_12170) - 2384500..2384679 (-) 180 WP_029726723.1 YqzE family protein -
  MHB62_RS12175 (MHB62_12175) comGG 2384751..2385125 (-) 375 WP_029726722.1 ComG operon protein ComGG Machinery gene
  MHB62_RS12180 (MHB62_12180) comGF 2385126..2385509 (-) 384 WP_032722118.1 ComG operon protein ComGF Machinery gene
  MHB62_RS12185 (MHB62_12185) comGE 2385535..2385882 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  MHB62_RS12190 (MHB62_12190) comGD 2385866..2386297 (-) 432 WP_029726720.1 comG operon protein ComGD Machinery gene
  MHB62_RS12195 (MHB62_12195) comGC 2386287..2386583 (-) 297 WP_029726719.1 comG operon protein ComGC Machinery gene
  MHB62_RS12200 (MHB62_12200) comGB 2386597..2387634 (-) 1038 WP_029317912.1 comG operon protein ComGB Machinery gene
  MHB62_RS12205 (MHB62_12205) comGA 2387621..2388691 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  MHB62_RS12210 (MHB62_12210) - 2388904..2389101 (-) 198 WP_014480259.1 CBS domain-containing protein -
  MHB62_RS12215 (MHB62_12215) corA 2389103..2390056 (-) 954 WP_004399136.1 magnesium transporter CorA -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14375.50 Da        Isoelectric Point: 5.8940

>NTDB_id=969265 MHB62_RS12180 WP_032722118.1 2385126..2385509(-) (comGF) [Bacillus sp. FSL K6-0255]
MLISGSLAAIIHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFEIYHSMIRKRVDG
KGHVPILDHITAMKADFENCVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=969265 MHB62_RS12180 WP_032722118.1 2385126..2385509(-) (comGF) [Bacillus sp. FSL K6-0255]
TTGCTCATATCAGGATCGTTAGCTGCGATTATCCATCTGTTTTTGTCTCGACAGCAGGAACATGACGGTTTCACACAGCA
AGAATGGATGATTTCGATAGAACAGATGATGAATGAATGCAAGGAGTCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGGCAGGACATCCGTTTCGAAATTTATCATTCAATGATAAGAAAAAGAGTGGATGGT
AAAGGGCATGTTCCGATTTTAGATCATATTACTGCCATGAAAGCTGATTTTGAAAATTGTGTTGTTTTGCTGAAAATTGA
GAGTGAGGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCGTATTTAGGAGGGGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

97.638

100

0.976

Multiple sequence alignment