Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MHI44_RS13560 Genome accession   NZ_CP150268
Coordinates   2573780..2574229 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus sp. FSL K6-1366     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2568780..2579229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI44_RS13520 (MHI44_13520) - 2569218..2569414 (+) 197 Protein_2617 hypothetical protein -
  MHI44_RS13525 (MHI44_13525) - 2569456..2569770 (+) 315 Protein_2618 RDD family protein -
  MHI44_RS13530 (MHI44_13530) - 2569852..2570184 (+) 333 WP_256683196.1 YckD family protein -
  MHI44_RS13535 (MHI44_13535) - 2570312..2570734 (+) 423 WP_174616579.1 MarR family transcriptional regulator -
  MHI44_RS13540 (MHI44_13540) - 2570722..2571063 (+) 342 WP_032724911.1 DUF3147 family protein -
  MHI44_RS13545 (MHI44_13545) - 2571082..2571812 (+) 731 Protein_2622 AAA family ATPase -
  MHI44_RS13550 (MHI44_13550) bglC 2571885..2573318 (+) 1434 WP_097080589.1 6-phospho-beta-glucosidase -
  MHI44_RS13555 (MHI44_13555) nin/comJ 2573355..2573753 (-) 399 WP_042977214.1 DNA-entry nuclease inhibitor Regulator
  MHI44_RS13560 (MHI44_13560) nucA/comI 2573780..2574229 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  MHI44_RS13565 (MHI44_13565) tlpC 2574397..2576118 (-) 1722 WP_339202139.1 methyl-accepting chemotaxis protein TlpC -
  MHI44_RS13570 (MHI44_13570) hxlB 2576229..2576786 (-) 558 WP_144483821.1 6-phospho-3-hexuloisomerase -
  MHI44_RS13575 (MHI44_13575) hxlA 2576792..2577424 (-) 633 WP_032724921.1 3-hexulose-6-phosphate synthase -
  MHI44_RS13580 (MHI44_13580) hxlR 2577656..2578018 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=968830 MHI44_RS13560 WP_003234608.1 2573780..2574229(-) (nucA/comI) [Bacillus sp. FSL K6-1366]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=968830 MHI44_RS13560 WP_003234608.1 2573780..2574229(-) (nucA/comI) [Bacillus sp. FSL K6-1366]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment