Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   ACK28Q_RS18220 Genome accession   NZ_CP176762
Coordinates   4023950..4025398 (-) Length   482 a.a.
NCBI ID   WP_014495831.1    Uniprot ID   A0ABV2RZM5
Organism   Bradyrhizobium japonicum strain DSM 3014     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4018950..4030398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK28Q_RS18195 (ACK28Q_18195) - 4019420..4020583 (-) 1164 WP_014495834.1 hypothetical protein -
  ACK28Q_RS18200 (ACK28Q_18200) - 4020662..4021402 (-) 741 WP_014495833.1 SDR family NAD(P)-dependent oxidoreductase -
  ACK28Q_RS18205 (ACK28Q_18205) - 4021473..4021604 (-) 132 WP_256461572.1 hypothetical protein -
  ACK28Q_RS18210 (ACK28Q_18210) purF 4021620..4023125 (-) 1506 WP_014495832.1 amidophosphoribosyltransferase -
  ACK28Q_RS18215 (ACK28Q_18215) - 4023154..4023783 (-) 630 WP_011086837.1 CvpA family protein -
  ACK28Q_RS18220 (ACK28Q_18220) radA/sms 4023950..4025398 (-) 1449 WP_014495831.1 DNA repair protein RadA Machinery gene
  ACK28Q_RS18225 (ACK28Q_18225) - 4025601..4026758 (+) 1158 WP_225894873.1 ABC transporter substrate-binding protein -
  ACK28Q_RS18230 (ACK28Q_18230) - 4026758..4028836 (+) 2079 WP_028169587.1 putative bifunctional diguanylate cyclase/phosphodiesterase -
  ACK28Q_RS18235 (ACK28Q_18235) - 4028843..4029568 (-) 726 WP_014495828.1 sulfite exporter TauE/SafE family protein -
  ACK28Q_RS18240 (ACK28Q_18240) rpiB 4029673..4030119 (-) 447 WP_014495827.1 ribose 5-phosphate isomerase B -

Sequence

Protein


Download         Length: 482 a.a.        Molecular weight: 50566.64 Da        Isoelectric Point: 7.6917

>NTDB_id=968746 ACK28Q_RS18220 WP_014495831.1 4023950..4025398(-) (radA/sms) [Bradyrhizobium japonicum strain DSM 3014]
MAKNTLSYVCQNCGAAYNRWQGKCESCGEWNTLAEEDTSGSVPVSIRSKRKGRTFALESLAGKSPDAPRLSSGMTELDRV
TGGGFVRGSVLLVGGDPGIGKSTLLTQATSMMARAGHRIVYISGEEAVAQVRLRAERLGLSDAPVQLAAETSVEDIVSTL
SEGAVPRLIVIDSIQTMWTDTVESAPGTVTQVRASAQALIRFAKKTGAAIILVGHVTKDGQIAGPRVVEHMVDAVLSFEG
EGSQQFRILRAVKNRFGPTDEIGVFEMTGLGLREVTNPSELFLSERDLGTPGTAVFAGIEGTRPVLVELQALVAPTSLGT
PRRAVVGWDQSRLSMVLAVLEAHCGVKLSGHDVYLNVAGGLRINEPAADMAAAAALVSSLVNAQLPTDAVYFGEISLSGV
IRPVAQTPARLKEAVKLGFKRAVLPESARGGDAGGDAGLSLNAINSLTTLVAEIAARGSRRGEQSAPAEKNATPARFRRG
EG

Nucleotide


Download         Length: 1449 bp        

>NTDB_id=968746 ACK28Q_RS18220 WP_014495831.1 4023950..4025398(-) (radA/sms) [Bradyrhizobium japonicum strain DSM 3014]
ATGGCCAAGAACACGCTTTCCTACGTCTGCCAGAACTGCGGCGCGGCCTATAACCGCTGGCAGGGCAAATGCGAGTCCTG
CGGCGAGTGGAACACGCTTGCCGAGGAGGATACGTCCGGCAGCGTGCCGGTCTCGATCCGCTCCAAGCGTAAGGGGCGCA
CATTCGCGCTGGAGAGCCTCGCCGGCAAAAGCCCGGACGCTCCTCGCCTGTCCTCGGGCATGACCGAGCTCGACCGCGTC
ACCGGTGGCGGCTTCGTCCGCGGCTCGGTGCTGCTGGTCGGCGGCGATCCCGGCATCGGCAAGTCGACGCTGCTGACGCA
GGCCACCAGCATGATGGCGCGCGCCGGCCACCGCATCGTCTACATCTCCGGCGAAGAGGCGGTCGCCCAGGTGCGCCTGC
GCGCCGAGCGGCTCGGCCTGTCGGACGCGCCGGTGCAGCTGGCGGCCGAGACCTCCGTCGAGGATATCGTCTCGACGCTG
TCGGAAGGCGCGGTGCCGCGGCTGATCGTGATCGACTCGATCCAGACCATGTGGACCGACACGGTGGAATCCGCGCCCGG
CACGGTCACCCAGGTGCGCGCCTCGGCGCAGGCGCTGATCCGCTTCGCCAAGAAGACGGGTGCTGCCATCATCCTGGTCG
GCCATGTCACCAAGGATGGCCAGATCGCCGGCCCCCGCGTGGTCGAGCACATGGTCGATGCGGTGCTGTCCTTCGAGGGC
GAAGGCTCGCAGCAGTTCCGCATCCTGCGCGCCGTGAAAAACCGCTTCGGCCCGACCGACGAGATCGGCGTGTTCGAGAT
GACCGGCCTTGGCCTGCGCGAGGTCACCAATCCGTCCGAGTTGTTCCTGTCCGAGCGCGATCTGGGCACGCCGGGCACCG
CAGTCTTCGCGGGCATCGAGGGCACGAGGCCCGTTCTGGTCGAATTGCAGGCCTTGGTGGCGCCCACCTCGCTCGGCACC
CCGCGCCGGGCCGTAGTCGGCTGGGATCAGAGCAGGCTCTCCATGGTGCTGGCGGTGCTGGAGGCCCATTGCGGGGTCAA
GCTGTCCGGCCACGACGTCTATCTGAACGTCGCGGGCGGCCTGCGCATCAACGAGCCGGCGGCCGATATGGCCGCCGCGG
CCGCACTGGTTTCATCCCTGGTTAATGCGCAGTTACCCACCGATGCCGTCTATTTCGGCGAGATTTCGCTCTCCGGCGTC
ATCCGCCCGGTGGCGCAGACACCGGCGCGGCTCAAGGAAGCGGTCAAGCTTGGCTTCAAGCGCGCCGTGCTGCCCGAATC
GGCCCGGGGTGGCGATGCCGGCGGCGACGCCGGACTGTCCCTGAACGCGATCAACAGCCTGACGACGCTGGTGGCCGAGA
TCGCGGCCCGGGGCTCCCGCCGCGGCGAACAGAGCGCTCCGGCGGAGAAAAATGCCACACCGGCAAGATTCCGCCGTGGA
GAGGGTTAG

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(75-221)

Subunit ChlI of Mg-chelatase (ChlI)

(337-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.528

89.627

0.417
  radA Streptococcus mitis NCTC 12261

45.012

89.419

0.402
  radA Streptococcus pneumoniae Rx1

44.548

89.419

0.398
  radA Streptococcus pneumoniae D39

44.548

89.419

0.398
  radA Streptococcus pneumoniae R6

44.548

89.419

0.398
  radA Streptococcus pneumoniae TIGR4

44.548

89.419

0.398
  radA Streptococcus mitis SK321

44.548

89.419

0.398

Multiple sequence alignment