Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OO778_RS02470 Genome accession   NZ_AP026765
Coordinates   513397..514437 (+) Length   346 a.a.
NCBI ID   WP_005381015.1    Uniprot ID   A0A2I3C9Z7
Organism   Vibrio sp. STUT-A16     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508397..519437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO778_RS02440 (VspSTUT16_04050) - 509511..510113 (-) 603 WP_074191809.1 XTP/dITP diphosphatase -
  OO778_RS02445 (VspSTUT16_04060) - 510232..510663 (-) 432 WP_005393982.1 DUF4426 domain-containing protein -
  OO778_RS02450 (VspSTUT16_04070) yggU 510791..511081 (-) 291 WP_005393980.1 DUF167 family protein YggU -
  OO778_RS02455 (VspSTUT16_04080) - 511081..511638 (-) 558 WP_012841393.1 YggT family protein -
  OO778_RS02460 (VspSTUT16_04090) proC 511691..512509 (-) 819 WP_256935766.1 pyrroline-5-carboxylate reductase -
  OO778_RS02465 (VspSTUT16_04100) - 512667..513368 (-) 702 WP_053304144.1 YggS family pyridoxal phosphate-dependent enzyme -
  OO778_RS02470 (VspSTUT16_04110) pilT 513397..514437 (+) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  OO778_RS02475 (VspSTUT16_04120) pilU 514468..515574 (+) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OO778_RS02480 (VspSTUT16_04130) ruvX 515637..516062 (-) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  OO778_RS02485 (VspSTUT16_04140) - 516097..516660 (-) 564 WP_005393973.1 YqgE/AlgH family protein -
  OO778_RS02490 (VspSTUT16_04150) gshB 516789..517739 (-) 951 WP_006743090.1 glutathione synthase -
  OO778_RS02495 (VspSTUT16_04160) rsmE 517753..518484 (-) 732 WP_005393970.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OO778_RS02500 (VspSTUT16_04170) endA 518601..519296 (-) 696 WP_264910654.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38532.02 Da        Isoelectric Point: 6.5039

>NTDB_id=96840 OO778_RS02470 WP_005381015.1 513397..514437(+) (pilT) [Vibrio sp. STUT-A16]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=96840 OO778_RS02470 WP_005381015.1 513397..514437(+) (pilT) [Vibrio sp. STUT-A16]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTGAGAAAACTAGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGACTTCTCTTTTGAGTTGCATAACGTTGGTCGT
TTCCGTGTCAACGCTTTCAACCAATCTCGTGGCTGTGCGGCAGTATTCCGTACCATTCCAAGTAGCATTCCAACTTTGGA
AGAGCTGGAAGCTCCCGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTTACTGGCCCAACAGGTT
CTGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAATCGTAATCACAACAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATCAACCAGCGTGAAGTACACCGTGATACGCATAGTTTCCAAAA
CGCACTGCGCAGCGCATTGCGTGAAGACCCGGATGTCATTCTGGTTGGTGAGATGCGTGACAAAGAAACCATCAGCCTTG
CGTTAACGGCGGCGGAAACGGGGCACTTAGTCTTCGGCACGTTACACACTAGCTCAGCGGCAAAAACCATTGACCGTATT
ATCGATGTGTTCCCTGGCAGTGATAAAGACATGGTGCGATCGATGCTTTCTGAGTCCCTTCGTTCGGTTATCGCACAAAA
ATTATTGAAACGTAATGGTGGTGGCCGTATTGCATGCCATGAAATCATGATGGCAACGCCTGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAAACTGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCACAAGGTATGGTCGCTCGTGAAGAGGTCGATAGTAAGATCGGGTTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3C9Z7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

99.711

0.867
  pilT Vibrio cholerae strain A1552

86.957

99.711

0.867
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.429

95.087

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.399

0.671
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

91.908

0.486
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361

Multiple sequence alignment