Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACK2VW_RS02735 Genome accession   NZ_CP176678
Coordinates   564948..566381 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli O121 strain GTVSS-020     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 559948..571381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2VW_RS02700 (ACK2VW_02700) elbB 560651..561304 (+) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACK2VW_RS02705 (ACK2VW_02705) mtgA 561301..562029 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACK2VW_RS02710 (ACK2VW_02710) yrbL 562026..562658 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ACK2VW_RS02715 (ACK2VW_02715) npr 562872..563144 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACK2VW_RS02720 (ACK2VW_02720) rapZ 563141..563995 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ACK2VW_RS02725 (ACK2VW_02725) ptsN 564041..564520 (-) 480 WP_000183675.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACK2VW_RS02730 (ACK2VW_02730) hpf 564638..564925 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACK2VW_RS02735 (ACK2VW_02735) rpoN/rpoN1 564948..566381 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ACK2VW_RS02740 (ACK2VW_02740) lptB 566429..567154 (-) 726 WP_000224098.1 LPS export ABC transporter ATP-binding protein -
  ACK2VW_RS02745 (ACK2VW_02745) lptA 567161..567718 (-) 558 WP_000669787.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACK2VW_RS02750 (ACK2VW_02750) lptC 567687..568262 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACK2VW_RS02755 (ACK2VW_02755) kdsC 568259..568825 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACK2VW_RS02760 (ACK2VW_02760) kdsD 568846..569832 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACK2VW_RS02765 (ACK2VW_02765) yrbG 569846..570823 (-) 978 WP_000922859.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=968252 ACK2VW_RS02735 WP_000809051.1 564948..566381(-) (rpoN/rpoN1) [Escherichia coli O121 strain GTVSS-020]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=968252 ACK2VW_RS02735 WP_000809051.1 564948..566381(-) (rpoN/rpoN1) [Escherichia coli O121 strain GTVSS-020]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465

Multiple sequence alignment