Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MKY56_RS02000 Genome accession   NZ_CP150257
Coordinates   370844..371293 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus sp. FSL M8-0054     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365844..376293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY56_RS01960 (MKY56_01960) - 366287..366482 (+) 196 Protein_342 hypothetical protein -
  MKY56_RS01965 (MKY56_01965) - 366522..366836 (+) 315 Protein_343 RDD family protein -
  MKY56_RS01970 (MKY56_01970) - 366918..367250 (+) 333 WP_015252849.1 YckD family protein -
  MKY56_RS01975 (MKY56_01975) - 367378..367800 (+) 423 WP_174616579.1 MarR family transcriptional regulator -
  MKY56_RS01980 (MKY56_01980) - 367788..368129 (+) 342 WP_032724911.1 DUF3147 family protein -
  MKY56_RS01985 (MKY56_01985) - 368148..368876 (+) 729 WP_046380777.1 AAA family ATPase -
  MKY56_RS01990 (MKY56_01990) bglC 368949..370382 (+) 1434 WP_134982418.1 6-phospho-beta-glucosidase -
  MKY56_RS01995 (MKY56_01995) nin/comJ 370419..370817 (-) 399 WP_015252845.1 DNA-entry nuclease inhibitor Regulator
  MKY56_RS02000 (MKY56_02000) nucA/comI 370844..371293 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  MKY56_RS02005 (MKY56_02005) tlpC 371461..373182 (-) 1722 WP_048654750.1 methyl-accepting chemotaxis protein TlpC -
  MKY56_RS02010 (MKY56_02010) hxlB 373293..373850 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  MKY56_RS02015 (MKY56_02015) hxlA 373856..374488 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  MKY56_RS02020 (MKY56_02020) hxlR 374720..375082 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=968196 MKY56_RS02000 WP_003234608.1 370844..371293(-) (nucA/comI) [Bacillus sp. FSL M8-0054]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=968196 MKY56_RS02000 WP_003234608.1 370844..371293(-) (nucA/comI) [Bacillus sp. FSL M8-0054]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment