Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MKX71_RS05795 Genome accession   NZ_CP150256
Coordinates   1133172..1136876 (+) Length   1234 a.a.
NCBI ID   WP_339231841.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0071     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1128172..1141876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX71_RS05780 (MKX71_05780) - 1128620..1128946 (-) 327 WP_032728981.1 four-helix bundle copper-binding protein -
  MKX71_RS05785 (MKX71_05785) - 1129064..1129501 (-) 438 WP_003245806.1 ferritin family protein -
  MKX71_RS05790 (MKX71_05790) addB 1129685..1133185 (+) 3501 WP_339231840.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MKX71_RS05795 (MKX71_05795) addA 1133172..1136876 (+) 3705 WP_339231841.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MKX71_RS05800 (MKX71_05800) sbcD 1136949..1138124 (+) 1176 WP_339231843.1 exonuclease subunit SbcD -
  MKX71_RS05805 (MKX71_05805) sbcC 1138121..1141513 (+) 3393 WP_339231844.1 exonuclease subunit SbcC -
  MKX71_RS05810 (MKX71_05810) hlpB 1141527..1141829 (+) 303 WP_015252406.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141273.74 Da        Isoelectric Point: 5.1477

>NTDB_id=968130 MKX71_RS05795 WP_339231841.1 1133172..1136876(+) (addA) [Bacillus sp. FSL M8-0071]
MNIPKPADTTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVQRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPGAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFSELYKRVPAVSFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLAEMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAENDKGERGPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAEDTDASEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLNTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
LGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDMETLAENGVTARPDISGHPARFAVRMIHSYD
LLDDDLEERMEEKSERLEAIRQGEPVPGSFMFDEKAREQLNWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSMLYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAEQTVHKLYEKELLTEEQKAAIDIEEIVQFFHTEIGG
QLISAQWKNREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETDGGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=968130 MKX71_RS05795 WP_339231841.1 1133172..1136876(+) (addA) [Bacillus sp. FSL M8-0071]
ATGAACATTCCTAAACCGGCAGACACCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCTGCGGGCTCTGGTAAAACCGCTGTGCTCGTTGAACGAATGATTCGGAAAATCACCGCGGAGGAAAACC
CAATAGATGTAGACCGTCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAGCACCGAATCGCAGAAGCC
TTGGAAAAAGAGCTTGTACAGCGCCCCGGCTCGCTGCATATTAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACGCTCCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATAGCTG
ATCAGACGGAAGGCGAGCTGCTCGGGGATGAAGTGCTTGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTATACGACAGACCGGCATGATCTGGATCTGCAATTTCTCGTTAAACAGGTGTACGAGTA
TTCCCGATCCCATCCCAACCCGGGGGCGTGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCAGAAAAGAGCGCCATCG
AGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCTCAGATTGATGAACT
GATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGCCGTCTCTTTTAAGCGTGCCAAAGCAGTAAAAG
GGGATGAGTTCGATCCAGCGCTCCTTGATGAGGCGACAGATTTGAGAAACGGCGCAAAAAAACTGCTTGAAAAGCTCAAA
ACCGACTACTTCACGCGAAGTCCTGAACAGCACTTGAAAAGCCTAGCCGAGATGAAGCCTGTCATTGAAACGCTCGTACA
GCTTGTCATCAGCTATGGAAAACGATTCGAAGCTGCGAAACAGGAAAAATCAATCATCGATTTTTCTGATTTGGAGCATT
ACTGTTTAGCGATTTTGACAGCTGAGAATGACAAAGGTGAACGTGGGCCGAGCGAGGCTGCAAGGTTTTATCAGGAACAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTCGTGCAGGAATCGATTCTGCAGCTTGTCACAAGCGGGCC
GGAGGAGACTGGTAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCTAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACGGGGCGGAAAATCGATTTAAATAAAAATTTCCGAAGC
CGGGCTGATATTTTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTCGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCAGCGTATCCGGACAATGACGAGACGGAAACAGAGCTGCTGCTGATCGACAACGCAG
AAGATACGGATGCAAGCGAGGAAGCAGAAGAGCTTGAAACGGTGCAGTTCGAGGCCAAAGCCATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACATCGCAATATTCAATACCGGGATATCGTGAT
TTTGCTCCGCTCCATGCCTTGGGCTCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTGA
CGTCAGGCTATTTCGAAGCGGTCGAAGTCGCCGTCGCGCTTTCTGTGCTGAAGGTAATCGATAATCCGTATCAGGATATA
CCGCTTGCCTCTGTGCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAGGCGATGAAAGACTACCTGGCTGCTGGTGACCGGAACGATGAGCTTTATCAAAAGCTAAATACGT
TTTACGGACATCTGCAAAAATGGCGCGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATATATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGCGTCCTTTACGACCGGGC
GCGCCAATATGAATCAACGGCGTTTCGCGGCCTGTTCCGTTTCCTGCGATTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAGGGGCTTGAA
TTCCCTGTTGTGTTTGCAGCGGGTCTCGGCCGCAATTTCAACATGATGGACTTAAACAAATCGTACCTGCTGGACAAGGA
GCTCGGGTTTGGCACGAAGTATATTCACCCGCAATTGCGTATCAGCTATCCGACGCTTCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGGGTACTCTATGTTGCATTAACAAGAGCAAAGGAAAAATTGTTTCTG
CTTGGCTCGTGCAAGGATCATCAGAAACAGCTTGCAAAATGGCAGGCATCTGCGTCACAAACTGATTGGCTTCTTCCGGA
GTTTGACCGCTATCAGGCAAAAACGTATTTAGATTTCATCGGGCCGGCTCTTGCCAGACACAGAGATATGGAGACTTTGG
CTGAAAACGGTGTGACGGCGCGTCCTGATATATCAGGACACCCGGCCCGTTTTGCCGTCCGAATGATCCATTCCTATGAT
TTGCTGGATGATGATTTGGAGGAAAGAATGGAAGAAAAAAGCGAGCGCCTAGAAGCGATTCGCCAAGGTGAACCAGTTCC
CGGCTCGTTTATGTTTGATGAAAAAGCCCGCGAGCAGCTGAACTGGACCTACCCGCATCACGAGGTGACGCAAATTCGCA
CAAAGCAGTCGGTGTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCTGTTAAACCGGCT
GACGGCAGCATGCTGTACAGACGGCCTGCTTTCATGATGAAAAAAGGCCTGACGGCGGCAGAGAAAGGAACGGCCATGCA
TACGGTGATGCAGCATATCCCGCTGACACATGTGCCGACGATAGAAGAAGCGGAGCAGACGGTTCATAAGCTTTATGAAA
AAGAACTGCTCACGGAAGAACAAAAAGCCGCTATAGATATAGAAGAAATCGTGCAATTCTTCCATACGGAAATCGGCGGA
CAGCTGATCAGCGCGCAATGGAAGAATAGGGAAGTACCGTTCAGTTTGGCGCTTCCAGCCAAGGAGATCTATCCTGATGC
ACAGGAGGCGGATGAGCCGCTTTTAGTGCAGGGAATCATTGATTGTCTCTATGAAACTGACGGCGGTTTATATCTTTTGG
ACTATAAATCGGACCGGATTGAGGGCAAATTCCAGCACGGATTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-880)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

97.245

100

0.972

Multiple sequence alignment