Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MKY10_RS01975 Genome accession   NZ_CP150253
Coordinates   369748..370197 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus sp. FSL P4-0290     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 364748..375197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY10_RS01940 (MKY10_01940) - 364899..365579 (-) 681 WP_024572176.1 amino acid ABC transporter permease -
  MKY10_RS01945 (MKY10_01945) - 365589..366452 (-) 864 WP_029317191.1 transporter substrate-binding domain-containing protein -
  MKY10_RS01950 (MKY10_01950) - 366613..366810 (+) 198 WP_128992271.1 hypothetical protein -
  MKY10_RS01955 (MKY10_01955) - 366830..367285 (+) 456 WP_048654748.1 RDD family protein -
  MKY10_RS01960 (MKY10_01960) - 367367..367699 (+) 333 WP_015252849.1 YckD family protein -
  MKY10_RS01965 (MKY10_01965) bglC 367853..369286 (+) 1434 WP_048654749.1 6-phospho-beta-glucosidase -
  MKY10_RS01970 (MKY10_01970) nin/comJ 369323..369721 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  MKY10_RS01975 (MKY10_01975) nucA/comI 369748..370197 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  MKY10_RS01980 (MKY10_01980) tlpC 370365..372078 (-) 1714 Protein_346 methyl-accepting chemotaxis protein TlpC -
  MKY10_RS01985 (MKY10_01985) hxlB 372189..372746 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  MKY10_RS01990 (MKY10_01990) hxlA 372752..373384 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  MKY10_RS01995 (MKY10_01995) hxlR 373613..373975 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=967927 MKY10_RS01975 WP_003234608.1 369748..370197(-) (nucA/comI) [Bacillus sp. FSL P4-0290]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=967927 MKY10_RS01975 WP_003234608.1 369748..370197(-) (nucA/comI) [Bacillus sp. FSL P4-0290]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment