Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MKY43_RS13100 Genome accession   NZ_CP150249
Coordinates   2532322..2533092 (-) Length   256 a.a.
NCBI ID   WP_003183624.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0133     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2527322..2538092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY43_RS13080 (MKY43_13080) - 2527397..2528209 (-) 813 WP_003183615.1 pyruvate, water dikinase regulatory protein -
  MKY43_RS13085 (MKY43_13085) - 2528235..2528873 (-) 639 WP_025804968.1 helix-turn-helix transcriptional regulator -
  MKY43_RS13090 (MKY43_13090) glyS 2529071..2531110 (-) 2040 WP_009327872.1 glycine--tRNA ligase subunit beta -
  MKY43_RS13095 (MKY43_13095) glyQ 2531103..2531990 (-) 888 WP_003183621.1 glycine--tRNA ligase subunit alpha -
  MKY43_RS13100 (MKY43_13100) recO 2532322..2533092 (-) 771 WP_003183624.1 DNA repair protein RecO Machinery gene
  MKY43_RS13105 (MKY43_13105) - 2533130..2533273 (-) 144 WP_003183626.1 YqzL family protein -
  MKY43_RS13110 (MKY43_13110) era 2533435..2534340 (-) 906 WP_009327871.1 GTPase Era -
  MKY43_RS13115 (MKY43_13115) - 2534321..2534731 (-) 411 WP_003183631.1 cytidine deaminase -
  MKY43_RS13120 (MKY43_13120) - 2534863..2535243 (-) 381 WP_003183633.1 diacylglycerol kinase family protein -
  MKY43_RS13125 (MKY43_13125) ybeY 2535221..2535697 (-) 477 WP_003183635.1 rRNA maturation RNase YbeY -
  MKY43_RS13130 (MKY43_13130) - 2535694..2537748 (-) 2055 WP_223307119.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29473.12 Da        Isoelectric Point: 8.5644

>NTDB_id=967705 MKY43_RS13100 WP_003183624.1 2532322..2533092(-) (recO) [Bacillus sp. FSL M8-0133]
MLTKCEGIVLRTNDYGETNKIITLLTREHGKIGVLARGAKKPNSRLSAISQPFLYASFLFRKSSGLGTLEQGEMIESMRN
IREDLFLTAYAAYIAELIDKGTEEKKPNPFLFELLLQSMRRLNDGTDPDIIANLIEVKMLSVMGLYPELDECVHCKSKDG
TFHFSIRDNGFICHRCFAKDPYRIPLSPASARLLRLFFYFDLSRLGNVSVKQETKDELKRVIQTYYDEYSGIYLKSRRFM
EQMDNMKKMLGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=967705 MKY43_RS13100 WP_003183624.1 2532322..2533092(-) (recO) [Bacillus sp. FSL M8-0133]
ATGCTGACGAAATGTGAAGGTATCGTCCTTCGTACAAACGACTATGGTGAAACAAATAAGATCATTACGCTATTGACGAG
AGAACACGGGAAAATCGGTGTGCTGGCAAGGGGCGCCAAAAAGCCGAACAGCCGCTTGTCAGCGATTTCCCAGCCGTTCC
TTTATGCATCTTTTTTATTCCGCAAATCATCCGGCCTCGGCACATTGGAGCAGGGCGAGATGATCGAAAGCATGAGGAAT
ATCCGCGAGGATCTGTTTTTAACAGCTTACGCGGCCTACATCGCTGAATTAATCGACAAGGGAACGGAGGAAAAGAAACC
GAATCCGTTTTTATTTGAGCTGCTGCTTCAGTCAATGAGGAGGCTGAATGACGGTACAGATCCCGACATTATCGCCAACC
TCATCGAAGTCAAAATGCTCAGCGTGATGGGGCTGTATCCGGAGCTGGATGAATGTGTGCACTGCAAAAGCAAAGACGGC
ACATTTCATTTTTCGATCCGCGACAACGGCTTTATCTGCCACCGCTGTTTTGCGAAAGATCCATACAGGATACCGCTGTC
TCCCGCATCCGCCAGGCTGTTGAGACTGTTTTTTTATTTTGACCTGTCAAGGCTCGGAAATGTCTCGGTCAAACAGGAGA
CGAAGGATGAGCTGAAAAGGGTTATTCAGACGTACTATGACGAGTACTCAGGGATCTACCTGAAATCGCGGCGCTTCATG
GAACAAATGGACAATATGAAAAAGATGCTGGGCGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

78.125

100

0.781

Multiple sequence alignment