Detailed information NTG0967561

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: MKY61_RS06255
• Genome accession: NZ_CP150247
• Coordinates: 1234652..1238344 (+)
• Length: 1230 a.a.
• NCBI ID: WP_071583744.1
• Uniprot ID: -
• Organism: Bacillus sp. FSL M8-0315
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1229652..1243344

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKY61_RS06245 (MKY61_06245)	modB	1230399..1231055 (+)	657	WP_009328900.1	molybdate ABC transporter permease subunit	-
MKY61_RS06250 (MKY61_06250)	addB	1231165..1234665 (+)	3501	WP_009328899.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
MKY61_RS06255 (MKY61_06255)	addA	1234652..1238344 (+)	3693	WP_071583744.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
MKY61_RS06260 (MKY61_06260)	sbcD	1238409..1239590 (+)	1182	WP_011197744.1	exonuclease subunit SbcD	-
MKY61_RS06265 (MKY61_06265)	sbcC	1239587..1242979 (+)	3393	WP_011197745.1	exonuclease subunit SbcC	-
MKY61_RS06270 (MKY61_06270)	-	1243023..1243241 (-)	219	WP_009328895.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141106.95 Da
• Isoelectric Point: 5.5198

>NTDB_id=967561 MKY61_RS06255 WP_071583744.1 1234652..1238344(+) (addA) [Bacillus sp. FSL M8-0315]
MEISKPKGSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQERPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYLIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKGNPA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPEGWLHSLAELYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPGGPAPRAENFLDDLAQIDRLIQQQDDFSALHELVPTVSFQRLKPCKGDEYDPRLVDEAADLRNSAKKQLEKLK
SDYFSRTPEQHLESLREMKPVIQTLVQLVLEYGRRFAEAKKEKAIVDFSDLEHDCLAILSVKNPAGEAVPSEAAKFYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPPNEATKTELLLIETPSGAPGEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAMKAYLAAADGDERLSEKLRRFDGLLKKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRSLYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRTQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPPVIDEIREHPARFQVSWLSAADLQAE
AVDQAGEERHDRLVQIQMGHAIEGAFEYEQQVRERLAWSYPYKDAAKVRTKQSVSEMKRQKEYEDEYGDRSLIRPSQEAL
LFKRPSFMMAKGLTAAERGTAMHTVMQHIPLTRTPEKNELSRLLDRLVEKELLTEDQRAAIEEDDILAFFDTEIGQKLFG
ARRVEREVPFNMTLSAKEVYPDLESADEPVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQ
LYARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide

• Length: 3693 bp

>NTDB_id=967561 MKY61_RS06255 WP_071583744.1 1234652..1238344(+) (addA) [Bacillus sp. FSL M8-0315]
ATGGAGATTTCTAAACCGAAGGGCAGCACCTGGACTGATGACCAATGGAAGGCGATTGTCTCATCCGGCCGGGACATTTT
AGTTGCGGCAGCGGCCGGTTCCGGAAAAACGGCCGTGCTGGTTGAACGGATCATTCGGAAAATAACGGATCAGGAACGAC
CGGTAGATGTCGACAGGCTGCTCGTCGTCACCTTTACAAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGATCGCTTCATTTGCGCCGACAGCTTGCTTTATTAAACAAAGCGAGCATATC
AACATTGCATTCATTCTGTCTTCAAGTCATCCGCAAATATTACTATTTGATCGATGTCGATCCGGCTTTCCGGATCGCCG
ATCAGACGGAAGGCGAGCTGCTCGGCGATGAGGTGCTGGATGAACTGTTTGAAGAAGAATACAAAAAAGGCAATCCTGCC
TTTTTTGAACTTGTAGACAGATATACGACAGACCGTCATGACCTCGACCTGCAGCATCTTGTCAAGCGGGTCTACGAATT
TTCCCGTTCGCATCCCGATCCGGAGGGCTGGCTTCACTCATTGGCTGAACTGTATGACGCGGCTTCGGATACGAAGGTTG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTCGTTCTGGAAGGTATGCGGCAAAAGCTGACGCGCGCG
CTTGATTTGACGAAGCAGCCCGGGGGGCCTGCTCCGCGCGCCGAAAATTTTCTCGATGATTTAGCGCAGATCGACAGGCT
GATTCAGCAGCAGGATGATTTTTCGGCCCTGCATGAGCTTGTACCGACCGTTTCGTTTCAACGATTGAAGCCATGCAAAG
GTGATGAGTACGATCCCAGATTGGTTGATGAGGCGGCAGACCTCCGCAACAGTGCGAAAAAGCAGCTTGAAAAGCTGAAA
AGCGATTATTTTTCGCGAACTCCGGAGCAGCACCTTGAGAGCCTGAGGGAAATGAAGCCTGTCATTCAGACGCTTGTCCA
GCTCGTGCTGGAGTATGGCAGGCGGTTTGCGGAAGCGAAAAAGGAAAAGGCGATCGTCGATTTTTCAGACCTTGAACACG
ACTGCCTTGCGATTTTATCCGTGAAAAATCCGGCGGGCGAGGCCGTGCCGAGCGAAGCGGCGAAGTTTTACCGCCATCAG
TTCCACGAAGTGCTTGTTGATGAATACCAGGATACGAACCTGGTCCAGGAAGCGATTTTAAAGCTCGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAGCAGTCGATTTACCGGTTCCGCCTGGCTGAGCCGCTTTTGTTCC
TCTCGAAATATAAACGCTTTACAGACGATGGCTCGGGTTCCGGCCAGAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCAGATATTTTGGACAGCACGAACTTTTTATTCAAACAGCTCATGGGAGAAAAAGTCGGGGAAGTCGAGTATGACGAACA
AGCCGAATTGAAGCTCGGCGCCTCTTATCCGCCGAACGAAGCGACAAAAACGGAGCTTCTGCTCATCGAAACCCCGAGCG
GCGCGCCGGGTGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGGCAGATCCGCAGGCTG
ATTACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCGCGAAACATCCAGTACCGTGATATCGTGGTGCTTTT
GCGGTCGATGCCTTGGGCGCCTCAAATTATGGACGAGTTTAAACAGCAGGGAATACCCGTATACGCGAACCTCTCAACTG
GTTATTTTGAGGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCCTATCAGGACATCCCGCTT
GCGTCCGTTCTCAGATCGCCTGTTGTGGGTCTTGATGAGAATGAGCTTTCCTTGATCAGAATCAAAGATAAAAAAGCGCC
GTTTTACGAAGCCATGAAAGCGTACCTTGCTGCAGCAGACGGCGATGAGAGGCTCAGCGAAAAGCTTCGCCGCTTTGACG
GTCTGCTCAAGAAGTGGAGAGCTTATGCGAAAAACCACTCTGTAGCAGAGCTGATCTGGGAAATCTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCTCGCTTTACGACAGGGCGAGATC
CTATGAAGCGACATCATTCCGCGGCTTGTTCCGCTTTTTAAGATTTATTGAGCGGATGCAGGAGCGGGGCGATGACCTCG
GAACGGCAAGGGCTCTAAGCGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGGCTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACTTGAACAAATCTTATCTGCTGGATAAAGAGCTCGG
TTTTGGAACGAAGTTTATCCATCCGAAGTGGCGGATCAGCTATCCGACGCTGCCGCTTATCGCGATGAAGAAAAAACTCC
GCCGCGAGCTTTTATCAGAGGAGCTGCGCGTCTTGTATGTCGCCCTTACCCGGGCCAAGGAAAAGCTGTACCTGGTCGGG
ACGGCGAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGACACAAGCGGCCGGGTCCGAGTGGCTGCTCCCGGATTATGA
ACGGTTTCAGGCAAAATCATATCTGGATTTCATCGGACCTGCTTTGATGAGGCACAGAGATATGGATGAATCCGGGGCGC
CGCCTGTGATTGATGAGATCCGCGAACATCCGGCGCGCTTTCAGGTCAGCTGGCTGTCCGCCGCGGATCTACAAGCTGAG
GCTGTCGATCAGGCCGGTGAAGAAAGACACGACCGGCTCGTCCAAATTCAAATGGGCCATGCGATTGAAGGCGCCTTTGA
ATATGAGCAGCAAGTCAGAGAACGACTGGCATGGTCATATCCATACAAGGACGCCGCCAAAGTCAGAACAAAACAATCTG
TCTCAGAAATGAAACGGCAGAAGGAATACGAGGATGAATACGGCGACCGCTCGCTTATTCGTCCATCACAGGAGGCTCTC
CTGTTTAAACGGCCGTCCTTTATGATGGCAAAAGGCCTGACCGCAGCCGAACGGGGGACGGCGATGCACACCGTCATGCA
GCATATTCCGCTGACCCGTACGCCTGAAAAGAATGAGCTCAGCCGCCTTCTAGACAGGCTTGTTGAGAAAGAGCTGTTAA
CAGAAGACCAGCGGGCAGCGATTGAAGAAGACGACATTCTTGCATTCTTTGATACGGAAATCGGACAAAAGCTTTTCGGC
GCCCGCCGCGTCGAACGGGAGGTTCCCTTTAACATGACGCTGTCCGCTAAAGAGGTTTATCCGGATCTTGAGTCAGCCGA
TGAACCTGTTTTGATACAGGGAATTATCGACTGCCTATTCGAGACGGAGGACGGTTTTTACCTTCTAGATTACAAAACCG
ACCGGATACACGGAAAGTACCGGAACGGCTTCGAAGGGGCCGAGCCGATTTTAAGAAAACGTTACGAAACACAAATTCAG
CTTTACGCACGAGCCGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGCGCGCTGTACTTTTTTGACGGAGGACACGT
GCTCTTATTTTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1051-1222)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	71.707	100	0.717