Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MKY61_RS06250 Genome accession   NZ_CP150247
Coordinates   1231165..1234665 (+) Length   1166 a.a.
NCBI ID   WP_009328899.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0315     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1226165..1239665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY61_RS06225 (MKY61_06225) - 1226824..1228191 (-) 1368 WP_071583743.1 HAMP domain-containing sensor histidine kinase -
  MKY61_RS06230 (MKY61_06230) - 1228188..1228862 (-) 675 WP_009328903.1 response regulator transcription factor -
  MKY61_RS06235 (MKY61_06235) - 1228952..1229392 (-) 441 WP_009328902.1 ferritin-like domain-containing protein -
  MKY61_RS06240 (MKY61_06240) modA 1229596..1230390 (+) 795 WP_011197742.1 molybdate ABC transporter substrate-binding protein -
  MKY61_RS06245 (MKY61_06245) modB 1230399..1231055 (+) 657 WP_009328900.1 molybdate ABC transporter permease subunit -
  MKY61_RS06250 (MKY61_06250) addB 1231165..1234665 (+) 3501 WP_009328899.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MKY61_RS06255 (MKY61_06255) addA 1234652..1238344 (+) 3693 WP_071583744.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MKY61_RS06260 (MKY61_06260) sbcD 1238409..1239590 (+) 1182 WP_011197744.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134408.49 Da        Isoelectric Point: 5.5700

>NTDB_id=967560 MKY61_RS06250 WP_009328899.1 1231165..1234665(+) (addB) [Bacillus sp. FSL M8-0315]
MGAVFLSGRSGSGKTTFILNEIREKLRDEPLGKPIIFLVPDQMTFLMEYELSKTPDLGGTIRAQVYSFSRLAWRVLQHTG
GMNRPFLTGTGIQMLLRRLIEEHKGEFKVYQNASDKSGFTEQVERMLTEFKRHCLPPQSIRDMMEGTGKSEYEDERILSD
KLHDLYILYSKLEENLENQYVQSEDYLTLLAEQIPYAEEIRNAAVYVDGFHQFTPQEMSVLEQLMVHAEEITFSLTADKP
FTANSPNDLHLFRMTGKAYYDLYQKAKELGLDPSEVRLEETKRHRHHPELQHLERYFDERPAKPYPGQTESLRIMQASNR
RTEIEGIAREIHSLIRQGRFRLRDIAVIARNVEDYKDTIKEVFKDCELPFFIDGKESMQNHPLIELIRSTLDIIKGNWRY
EAVFRCVKTELLFPEGQPKERLREQIDQLENYCIAYGIKGDRWTSKDPFVYRRYASLDEDFAKTDKEIETENMLNELKGW
IVPPIHRLQKRLKKAETVREMAEAVYLYLEEADVPMKLEQERRLAEEGGRIAESRQHEQVWDAVIQLLDEFVEMMGTERI
SFALFQQMIETGLESLKFALIPPALDQVFIGNMDLSRMYGTKCTFLIGVNDGILPARPADDGVLSDEDREWLKRNGAQLA
ATGREQLLDENFLIYMTLSSPSEKLYVSYPIADSEGKTLLPSTVVKRLNELFPDSEEKMLIHEPEQLDDEAQLEFLVNKG
IALSHLAGQLGIWTRQYAISDVWWSTYNFLMNEPDRIFSQNILSSLFFRNKVENLNRHVSRDLYGEHIQGSVSRMETFKA
CPFSHFASHGLKLKERQFFKLEAPDIGQLFHSALKLISDRLHELKLDWRDLTKAQCETLSSDAVERLAPKLQKEILLSSN
RHHYVKQKLQKIIARVSGILSEHAKASGFAPVGIELGFGGKGPLPPMRFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSTDWLGMKASPAGVLYFHVHDPMIQASVPLGLDEIEKEIFKKFKMKGL
LLGDQEAVKLMDTTLEQGRSNIISAGLKKDGSLRSDSDVVAEEDFHVLRRHIRRTFQQAGEEITDGKVSIEPYKLKDRTP
CTYCSYRSFCQFDESLEENEYRILKPEKDSVILERLKKEDEADGDF

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=967560 MKY61_RS06250 WP_009328899.1 1231165..1234665(+) (addB) [Bacillus sp. FSL M8-0315]
GTGGGAGCAGTATTTTTATCAGGCAGATCCGGGAGTGGGAAAACGACCTTTATCCTGAATGAAATCAGAGAAAAACTTCG
CGATGAACCGCTCGGAAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTAGCAAAA
CACCAGATTTGGGCGGCACCATCCGCGCCCAAGTGTACAGTTTTTCACGGCTTGCCTGGCGAGTGCTCCAACACACCGGA
GGCATGAACCGGCCGTTCTTGACGGGAACGGGCATTCAGATGCTGCTGCGGAGACTGATTGAAGAGCATAAGGGCGAGTT
TAAAGTCTATCAAAACGCAAGCGATAAAAGCGGATTTACGGAGCAGGTGGAACGGATGCTGACCGAATTTAAACGGCACT
GTCTGCCGCCTCAAAGCATCCGGGATATGATGGAAGGGACAGGCAAGTCGGAATACGAAGATGAACGGATTCTTTCCGAT
AAATTGCACGACTTATATATTTTGTACAGCAAGCTTGAGGAAAATCTGGAGAATCAATATGTTCAATCTGAAGATTATTT
GACCCTTTTAGCCGAACAGATTCCGTATGCGGAAGAGATTAGAAACGCTGCCGTTTACGTTGACGGATTTCACCAATTCA
CGCCTCAGGAGATGAGCGTACTAGAACAGCTGATGGTACATGCCGAAGAGATCACGTTTTCGCTGACGGCGGATAAGCCG
TTTACGGCAAACAGCCCGAATGATCTCCATCTGTTCAGGATGACCGGGAAGGCGTACTATGACCTTTACCAAAAGGCGAA
GGAACTCGGCCTTGATCCGTCTGAAGTGCGGCTGGAAGAAACGAAACGGCACCGGCATCATCCGGAGCTTCAGCATCTTG
AACGCTATTTTGATGAACGTCCTGCAAAACCGTATCCCGGACAGACGGAATCGCTCCGCATCATGCAGGCGTCGAACAGA
AGAACCGAGATTGAAGGAATTGCGCGCGAGATTCATTCTTTAATCCGCCAGGGCCGGTTCCGGCTTAGGGATATTGCTGT
CATCGCCCGCAATGTTGAAGATTATAAAGATACGATAAAAGAAGTTTTTAAAGACTGCGAACTTCCTTTTTTCATAGACG
GCAAGGAATCGATGCAAAATCATCCGCTTATTGAGCTGATCCGTTCGACGCTTGATATCATTAAAGGAAATTGGCGGTAT
GAAGCCGTGTTCCGCTGTGTCAAAACGGAGCTTTTATTCCCGGAAGGACAGCCGAAGGAACGGCTGCGCGAACAGATTGA
CCAGCTTGAAAACTACTGTATTGCTTATGGCATTAAAGGAGACAGATGGACAAGCAAAGATCCGTTTGTTTACAGAAGAT
ACGCTTCACTGGATGAGGATTTTGCCAAGACAGACAAAGAGATTGAAACGGAAAATATGCTCAATGAACTAAAGGGCTGG
ATCGTTCCGCCGATTCACAGGCTGCAGAAGCGCTTGAAAAAAGCTGAAACAGTAAGGGAAATGGCCGAAGCCGTCTACCT
TTATCTTGAAGAAGCCGATGTTCCGATGAAGCTTGAACAGGAAAGGCGGCTAGCCGAAGAAGGCGGAAGGATTGCCGAGT
CGCGCCAGCATGAGCAAGTATGGGATGCCGTTATTCAACTGTTGGACGAGTTTGTTGAAATGATGGGCACAGAGCGGATT
TCGTTTGCGTTATTTCAGCAAATGATCGAAACCGGATTGGAGTCTTTGAAATTCGCGCTGATTCCGCCCGCACTTGATCA
AGTGTTTATCGGCAATATGGACCTTTCAAGGATGTACGGCACGAAATGTACATTTCTCATCGGTGTAAACGACGGCATAC
TTCCGGCGAGGCCTGCGGATGACGGCGTGCTTTCAGACGAGGACAGGGAGTGGCTGAAACGAAACGGCGCACAGCTGGCG
GCAACAGGGCGAGAGCAGCTATTGGACGAGAATTTTCTGATCTATATGACGCTTTCAAGCCCGTCTGAAAAGCTTTATGT
CTCCTATCCGATCGCTGATTCGGAAGGGAAGACGCTGCTTCCTTCAACAGTGGTGAAACGGCTGAATGAACTGTTCCCGG
ACAGCGAAGAAAAGATGCTGATTCATGAACCCGAGCAGCTGGACGATGAAGCCCAGCTTGAATTTCTCGTGAATAAAGGC
ATCGCCTTGAGCCATTTGGCAGGGCAGCTCGGCATCTGGACGAGGCAGTATGCGATCAGCGATGTATGGTGGAGTACATA
CAACTTTTTAATGAATGAGCCTGACCGCATCTTTTCTCAAAACATCCTGTCCAGCCTGTTTTTCAGAAACAAGGTCGAAA
ACCTAAACCGGCATGTGAGCCGCGATTTATACGGGGAGCACATTCAGGGAAGCGTGTCGCGGATGGAGACGTTCAAGGCC
TGTCCGTTTTCCCATTTTGCTTCGCACGGCTTAAAGCTGAAGGAACGCCAATTTTTCAAGCTTGAAGCTCCGGATATCGG
TCAGCTGTTCCACTCTGCGCTGAAGCTGATTTCAGACCGGCTTCACGAGCTTAAACTGGATTGGCGCGACTTAACGAAAG
CCCAGTGCGAGACTTTGTCAAGCGATGCAGTTGAACGGCTTGCGCCTAAACTGCAAAAGGAGATTCTGCTCAGTTCGAAC
CGTCATCATTATGTGAAGCAGAAGCTGCAAAAAATCATTGCCAGGGTATCGGGCATTTTAAGTGAGCATGCAAAAGCAAG
CGGCTTTGCCCCCGTCGGCATAGAGCTCGGATTCGGAGGGAAAGGTCCGCTTCCTCCAATGCGTTTCACCTTGAAGAACG
GCTGCACGATGGAGCTTGTCGGCCGGATCGACAGAGTGGATAAAGCGGAGAGCTCAAAAGGGCTGCTGCTTAGGATTGTT
GATTATAAATCAAGCGATAAAGGCCTCGATTTGGCCGAGGTGTATTACGGGCTTGCCCTGCAAATGCTGACATATCTCGA
TTTGTCGATCACGCATTCAACCGATTGGCTCGGCATGAAAGCATCCCCGGCAGGCGTGCTCTATTTTCATGTGCACGATC
CAATGATCCAGGCTTCAGTGCCGCTCGGCCTTGATGAAATAGAAAAAGAGATTTTCAAAAAGTTCAAGATGAAAGGGCTG
CTCCTGGGAGACCAGGAAGCGGTCAAGCTGATGGACACCACCCTGGAACAAGGCAGGTCGAATATTATCAGCGCCGGTTT
GAAAAAAGACGGCTCGCTCCGTTCGGATTCTGACGTTGTTGCAGAAGAGGATTTTCATGTATTGAGGCGTCATATCCGCC
GGACATTTCAGCAGGCCGGCGAGGAGATCACAGACGGCAAAGTGTCGATCGAGCCTTACAAGCTAAAAGACCGGACACCG
TGCACATATTGTTCATACCGGTCATTCTGCCAGTTTGACGAATCGCTTGAGGAAAACGAATACCGAATATTAAAACCTGA
AAAAGACAGCGTGATATTGGAGCGGCTGAAAAAGGAGGATGAGGCAGATGGAGATTTCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

74.077

99.914

0.74

Multiple sequence alignment