Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MKX44_RS13975 Genome accession   NZ_CP150246
Coordinates   2650276..2651046 (-) Length   256 a.a.
NCBI ID   WP_003183624.1    Uniprot ID   -
Organism   Bacillus sp. FSL M8-0359     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2645276..2656046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKX44_RS13955 (MKX44_13955) - 2645351..2646163 (-) 813 WP_003183615.1 pyruvate, water dikinase regulatory protein -
  MKX44_RS13960 (MKX44_13960) - 2646189..2646827 (-) 639 WP_025804968.1 helix-turn-helix transcriptional regulator -
  MKX44_RS13965 (MKX44_13965) glyS 2647025..2649064 (-) 2040 WP_003183618.1 glycine--tRNA ligase subunit beta -
  MKX44_RS13970 (MKX44_13970) glyQ 2649057..2649944 (-) 888 WP_003183621.1 glycine--tRNA ligase subunit alpha -
  MKX44_RS13975 (MKX44_13975) recO 2650276..2651046 (-) 771 WP_003183624.1 DNA repair protein RecO Machinery gene
  MKX44_RS13980 (MKX44_13980) - 2651084..2651227 (-) 144 WP_003183626.1 YqzL family protein -
  MKX44_RS13985 (MKX44_13985) era 2651389..2652294 (-) 906 WP_009327871.1 GTPase Era -
  MKX44_RS13990 (MKX44_13990) - 2652275..2652685 (-) 411 WP_003183631.1 cytidine deaminase -
  MKX44_RS13995 (MKX44_13995) - 2652817..2653197 (-) 381 WP_003183633.1 diacylglycerol kinase family protein -
  MKX44_RS14000 (MKX44_14000) ybeY 2653175..2653651 (-) 477 WP_003183635.1 rRNA maturation RNase YbeY -
  MKX44_RS14005 (MKX44_14005) - 2653648..2655702 (-) 2055 WP_228767688.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29473.12 Da        Isoelectric Point: 8.5644

>NTDB_id=967516 MKX44_RS13975 WP_003183624.1 2650276..2651046(-) (recO) [Bacillus sp. FSL M8-0359]
MLTKCEGIVLRTNDYGETNKIITLLTREHGKIGVLARGAKKPNSRLSAISQPFLYASFLFRKSSGLGTLEQGEMIESMRN
IREDLFLTAYAAYIAELIDKGTEEKKPNPFLFELLLQSMRRLNDGTDPDIIANLIEVKMLSVMGLYPELDECVHCKSKDG
TFHFSIRDNGFICHRCFAKDPYRIPLSPASARLLRLFFYFDLSRLGNVSVKQETKDELKRVIQTYYDEYSGIYLKSRRFM
EQMDNMKKMLGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=967516 MKX44_RS13975 WP_003183624.1 2650276..2651046(-) (recO) [Bacillus sp. FSL M8-0359]
ATGCTGACGAAATGTGAAGGTATCGTCCTTCGTACAAACGACTATGGTGAAACAAATAAGATCATTACGCTATTGACGAG
AGAACACGGGAAAATCGGTGTGCTGGCAAGGGGCGCCAAAAAGCCGAACAGCCGCTTGTCAGCGATTTCCCAGCCGTTCC
TTTATGCATCTTTTTTATTCCGCAAATCATCCGGCCTCGGCACATTGGAGCAGGGCGAGATGATCGAAAGCATGAGGAAT
ATCCGCGAGGATCTGTTTTTAACAGCTTACGCGGCCTACATCGCTGAATTAATCGACAAGGGAACGGAGGAAAAGAAACC
GAATCCGTTTTTATTTGAGCTGCTGCTTCAGTCAATGAGGAGGCTGAATGACGGTACAGATCCCGACATTATCGCCAACC
TCATCGAAGTCAAAATGCTCAGCGTGATGGGGCTGTATCCGGAGCTGGATGAATGTGTGCACTGCAAAAGCAAAGACGGC
ACATTTCATTTTTCGATCCGCGACAACGGCTTTATCTGCCACCGCTGTTTTGCGAAAGATCCATACAGGATACCGCTGTC
TCCCGCATCCGCCAGGCTGTTGAGACTGTTTTTTTATTTTGACCTGTCAAGGCTCGGAAATGTCTCGGTCAAACAGGAGA
CGAAGGATGAGCTGAAAAGGGTTATTCAGACGTACTATGACGAGTACTCAGGGATCTACCTGAAATCGCGGCGCTTCATG
GAACAAATGGACAATATGAAAAAGATGCTGGGCGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

78.125

100

0.781

Multiple sequence alignment