Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NST10_RS02445 Genome accession   NZ_CP150198
Coordinates   458620..459117 (+) Length   165 a.a.
NCBI ID   WP_001794055.1    Uniprot ID   A0A7U7EV69
Organism   Staphylococcus sp. FSL W8-0305     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453620..464117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST10_RS02405 (NST10_02405) - 453664..454650 (+) 987 WP_000161314.1 ROK family glucokinase -
  NST10_RS02410 (NST10_02410) - 454650..454979 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NST10_RS02415 (NST10_02415) - 454976..455599 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NST10_RS02420 (NST10_02420) comGA 455651..456625 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NST10_RS02425 (NST10_02425) comGB 456597..457667 (+) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  NST10_RS02430 (NST10_02430) comGC 457681..457992 (+) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  NST10_RS02435 (NST10_02435) comGD 457970..458416 (+) 447 WP_339211021.1 competence type IV pilus minor pilin ComGD Machinery gene
  NST10_RS02440 (NST10_02440) comGE 458403..458702 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  NST10_RS02445 (NST10_02445) comGF 458620..459117 (+) 498 WP_001794055.1 competence type IV pilus minor pilin ComGF Machinery gene
  NST10_RS02450 (NST10_02450) - 459214..459360 (+) 147 WP_001789879.1 hypothetical protein -
  NST10_RS02455 (NST10_02455) - 459350..459874 (+) 525 WP_001015125.1 shikimate kinase -
  NST10_RS02460 (NST10_02460) gcvT 460033..461124 (+) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  NST10_RS02465 (NST10_02465) gcvPA 461144..462490 (+) 1347 WP_303661271.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  NST10_RS02470 (NST10_02470) gcvPB 462483..463955 (+) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19224.98 Da        Isoelectric Point: 10.2192

>NTDB_id=966024 NST10_RS02445 WP_001794055.1 458620..459117(+) (comGF) [Staphylococcus sp. FSL W8-0305]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLNKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=966024 NST10_RS02445 WP_001794055.1 458620..459117(+) (comGF) [Staphylococcus sp. FSL W8-0305]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAACAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EV69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97

Multiple sequence alignment