Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NSQ22_RS00530 Genome accession   NZ_CP150187
Coordinates   98396..99769 (+) Length   457 a.a.
NCBI ID   WP_029761778.1    Uniprot ID   -
Organism   Geobacillus sp. FSL K6-3411     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 93396..104769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ22_RS00510 (NSQ22_00510) ctsR 93769..94230 (+) 462 WP_029761781.1 transcriptional regulator CtsR -
  NSQ22_RS00515 (NSQ22_00515) - 94245..94793 (+) 549 WP_008882051.1 UvrB/UvrC motif-containing protein -
  NSQ22_RS00520 (NSQ22_00520) - 94798..95889 (+) 1092 WP_011886620.1 protein arginine kinase -
  NSQ22_RS00525 (NSQ22_00525) clpC 95886..98321 (+) 2436 WP_029761780.1 ATP-dependent protease ATP-binding subunit ClpC -
  NSQ22_RS00530 (NSQ22_00530) radA 98396..99769 (+) 1374 WP_029761778.1 DNA repair protein RadA Machinery gene
  NSQ22_RS00535 (NSQ22_00535) - 99975..101069 (+) 1095 WP_008882047.1 PIN/TRAM domain-containing protein -
  NSQ22_RS00540 (NSQ22_00540) ispD 101091..101777 (+) 687 WP_029761777.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NSQ22_RS00545 (NSQ22_00545) ispF 101792..102274 (+) 483 WP_029761776.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  NSQ22_RS00550 (NSQ22_00550) gltX 102485..103954 (+) 1470 WP_029761775.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49445.10 Da        Isoelectric Point: 8.0108

>NTDB_id=965462 NSQ22_RS00530 WP_029761778.1 98396..99769(+) (radA) [Geobacillus sp. FSL K6-3411]
MAKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEIERTKPAVRGAFLHSEPSGPAKPIPITAVTAAQEPRIETKSVEL
NRVLGGGIVKGSLVLIGGDPGIGKSTLLLQTSAQLAAAGHTVLYISGEESVKQVKLRAGRLHAESHQLYVLAEADLEYIV
TAIETIQPACVIVDSIQTMYRTDITSAPGSVAQVRECTAELMKIAKTKGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEIGIFEMRDIGLREVENPSEVFLEERSRGAAGSTVVAAMEGTRPVLVEIQALVSPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDRPTNPADVIIGEVGLT
GEVRRVSRIEQRVQEAVKLGFSRVIVPKNNLTGWQPPAGVNVIGVSHVAEALEHTML

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=965462 NSQ22_RS00530 WP_029761778.1 98396..99769(+) (radA) [Geobacillus sp. FSL K6-3411]
ATGGCGAAAAAGAAAACAAAATTTGTTTGCCAAGAATGCGGGTATGAATCAGCGAAATGGCTCGGTCGTTGCCCGGGCTG
CCAAACGTGGAACTCGTTCGTTGAAGAAATCGAGCGGACAAAACCGGCTGTGCGCGGGGCGTTTCTTCATTCGGAACCAT
CGGGGCCAGCCAAACCAATTCCGATTACGGCTGTTACGGCAGCACAAGAGCCACGCATTGAAACGAAAAGTGTGGAGCTC
AATCGGGTGCTTGGCGGAGGAATCGTCAAAGGATCGCTCGTCTTAATCGGCGGTGATCCGGGTATTGGCAAATCGACGTT
ATTGCTGCAGACATCGGCACAGCTCGCTGCGGCCGGACATACGGTATTGTATATATCCGGTGAAGAGTCGGTGAAGCAAG
TGAAGCTGCGCGCTGGGCGTCTTCACGCTGAGTCTCATCAACTGTATGTGTTAGCAGAGGCGGATTTGGAATACATTGTA
ACAGCAATTGAAACGATTCAGCCTGCTTGTGTGATTGTCGATTCCATTCAGACGATGTACCGAACGGATATTACATCGGC
GCCGGGCAGTGTTGCCCAAGTGCGCGAGTGCACCGCTGAGCTAATGAAAATCGCCAAGACGAAAGGCATTGCCATTTTCA
TTGTTGGCCATGTGACCAAAGAAGGGGCGATCGCCGGGCCGCGCCTGCTTGAGCATATGGTTGATACCGTTCTTTATTTT
GAAGGGGAGCGGCATCATACGTACCGCATTTTGCGGGCGGTGAAAAACCGCTTCGGTTCAACGAATGAAATCGGTATTTT
TGAAATGCGTGACATCGGGCTGCGCGAAGTAGAAAATCCGTCGGAAGTGTTTTTGGAAGAACGGTCGCGCGGGGCGGCCG
GCTCGACAGTTGTCGCGGCAATGGAGGGGACGCGTCCGGTGCTTGTTGAAATTCAGGCGCTCGTTTCGCCAACGAGTTTC
GGCAACCCGCGACGGATGGCGACCGGCCTTGATCATAATCGCGTATCGCTGTTAATGGCTGTGCTCGAAAAGCGGGTCGG
GCTCCTGTTGCAAAACCAGGATGCTTACTTGAAAGTGGCAGGTGGAGTAAAGCTTGATGAACCGGCAATCGATTTGGCGG
TCGCCGTCAGCATTGCCTCGAGCTTCCGCGACCGGCCGACAAATCCAGCCGATGTCATTATCGGCGAAGTCGGCTTGACC
GGGGAAGTGCGCCGCGTTTCCCGCATTGAACAGCGCGTGCAAGAAGCCGTCAAATTAGGCTTTTCACGGGTCATTGTACC
AAAAAACAATTTGACTGGTTGGCAGCCGCCTGCAGGCGTCAATGTGATCGGTGTTTCGCATGTGGCCGAGGCGCTTGAGC
ATACGATGTTATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.617

100

0.746
  radA Streptococcus mitis NCTC 12261

65.121

99.125

0.646
  radA Streptococcus mitis SK321

64.901

99.125

0.643
  radA Streptococcus pneumoniae Rx1

64.68

99.125

0.641
  radA Streptococcus pneumoniae D39

64.68

99.125

0.641
  radA Streptococcus pneumoniae R6

64.68

99.125

0.641
  radA Streptococcus pneumoniae TIGR4

64.68

99.125

0.641

Multiple sequence alignment