Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NSQ81_RS12530 Genome accession   NZ_CP150163
Coordinates   2445399..2445782 (-) Length   127 a.a.
NCBI ID   WP_003230168.1    Uniprot ID   -
Organism   Bacillus sp. PS65     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2440399..2450782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ81_RS12490 (NSQ81_12490) sinI 2441333..2441506 (+) 174 WP_003230187.1 anti-repressor SinI family protein Regulator
  NSQ81_RS12495 (NSQ81_12495) sinR 2441540..2441875 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  NSQ81_RS12500 (NSQ81_12500) tasA 2441968..2442753 (-) 786 WP_004398632.1 biofilm matrix protein TasA -
  NSQ81_RS12505 (NSQ81_12505) - 2442817..2443389 (-) 573 WP_003246088.1 signal peptidase I -
  NSQ81_RS12510 (NSQ81_12510) tapA 2443373..2444134 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  NSQ81_RS12515 (NSQ81_12515) - 2444406..2444732 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  NSQ81_RS12520 (NSQ81_12520) - 2444774..2444953 (-) 180 WP_003230176.1 YqzE family protein -
  NSQ81_RS12525 (NSQ81_12525) comGG 2445024..2445398 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  NSQ81_RS12530 (NSQ81_12530) comGF 2445399..2445782 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  NSQ81_RS12535 (NSQ81_12535) comGE 2445808..2446155 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  NSQ81_RS12540 (NSQ81_12540) comGD 2446139..2446570 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  NSQ81_RS12545 (NSQ81_12545) comGC 2446560..2446856 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  NSQ81_RS12550 (NSQ81_12550) comGB 2446870..2447907 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  NSQ81_RS12555 (NSQ81_12555) comGA 2447894..2448964 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  NSQ81_RS12560 (NSQ81_12560) corA 2449376..2450329 (-) 954 WP_004399136.1 magnesium transporter CorA -

Sequence

Protein


Download         Length: 127 a.a.        Molecular weight: 14281.37 Da        Isoelectric Point: 5.8929

>NTDB_id=964322 NSQ81_RS12530 WP_003230168.1 2445399..2445782(-) (comGF) [Bacillus sp. PS65]
MLISGSLAAIIHLFLSRQQEHDGFTQQEWMISIEQMMNECKESQAVKTAEHGSVLICTNLSGQDIRFDIYHSMIRKRVDG
KGHVPILDHITAMKADIENGVVLLKIESEDQKVYQTAFPVYSYLGGG

Nucleotide


Download         Length: 384 bp        

>NTDB_id=964322 NSQ81_RS12530 WP_003230168.1 2445399..2445782(-) (comGF) [Bacillus sp. PS65]
TTGCTCATATCAGGATCGTTAGCTGCGATTATCCATCTGTTTTTGTCTCGACAGCAGGAACATGACGGTTTCACACAGCA
GGAATGGATGATTTCGATAGAACAGATGATGAATGAATGCAAGGAATCACAGGCAGTTAAGACAGCCGAGCATGGGAGCG
TGTTAATCTGCACCAATCTTTCCGGACAAGACATCCGTTTTGACATTTATCATTCAATGATAAGAAAAAGAGTGGATGGC
AAAGGGCATGTTCCGATTTTAGATCATATTACTGCCATGAAAGCTGATATTGAAAATGGTGTTGTTTTGCTGAAAATTGA
GAGTGAAGACCAAAAAGTGTATCAAACTGCTTTTCCAGTCTATTCGTATTTAGGAGGGGGGTGA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(16-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment