Detailed information NTG0964197

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACJEIC_RS06490
• Genome accession: NZ_CP174240
• Coordinates: 1426918..1428402 (-)
• Length: 494 a.a.
• NCBI ID: WP_011215352.1
• Uniprot ID: I7HJU3
• Organism: Legionella pneumophila strain SPF585
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1421918..1433402

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJEIC_RS06475 (ACJEIC_06475)	cysS	1422919..1424289 (+)	1371	WP_011215349.1	cysteine--tRNA ligase	-
ACJEIC_RS06480 (ACJEIC_06480)	-	1424461..1426374 (+)	1914	WP_011215350.1	DUF3413 domain-containing protein	-
ACJEIC_RS06485 (ACJEIC_06485)	-	1426503..1426817 (+)	315	WP_011215351.1	DUF6482 family protein	-
ACJEIC_RS06490 (ACJEIC_06490)	pilB	1426918..1428402 (-)	1485	WP_011215352.1	GspE family T2SS ATPase variant LspE	Machinery gene
ACJEIC_RS06495 (ACJEIC_06495)	lspD	1428406..1430781 (-)	2376	WP_070322060.1	GspD family T2SS secretin variant LspD	-
ACJEIC_RS06500 (ACJEIC_06500)	-	1430954..1431982 (+)	1029	WP_011215354.1	Gfo/Idh/MocA family protein	-

Sequence

Protein

• Length: 494 a.a.
• Molecular weight: 55023.40 Da
• Isoelectric Point: 6.0364

>NTDB_id=964197 ACJEIC_RS06490 WP_011215352.1 1426918..1428402(-) (pilB) [Legionella pneumophila strain SPF585]
MENEEKSLKIPYSFAKGNGLVVGEIKENLAIVYHLPNTPLQAFAEIKRLLQCELDLKQVDETTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYENRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPKHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRQEE
KELIGLKPDADVSQVFEPKGCDSCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLSG
DTSLAEILRVTSQN

Nucleotide

• Length: 1485 bp

>NTDB_id=964197 ACJEIC_RS06490 WP_011215352.1 1426918..1428402(-) (pilB) [Legionella pneumophila strain SPF585]
ATGGAAAATGAAGAAAAGTCACTTAAAATTCCTTACAGTTTTGCCAAAGGCAATGGACTTGTTGTGGGAGAAATAAAAGA
AAATCTGGCTATTGTCTATCACCTACCTAACACACCTCTGCAAGCGTTCGCCGAGATAAAGCGATTATTGCAATGCGAAC
TTGATCTAAAACAAGTTGATGAAACCACTTTTCAGCAACATTTAGCCAATATTTACCAATCTAAATCTTCAATACTGGAT
GCCGCAGAAGGCATGGAAGAAGATATGGATTTATCTATGTTAGCCAGTCAACTTCCTGTCAGCGAAGACTTATTGGAAAA
TCAAGACGATGCTCCAATTATCCGTTTACTGAATGCTCTGTTCACTCAAGCCATTAAGCAAAAAGCATCTGATATACACA
TCGAAACATATGAAAACAGAGTCTTGGTGCGCAACCGAATTGATGGTGTTCTACAAGAGGTCCTTGAAATTCAACGAGCT
ATCGCTCCTTTGGTCATTTCCAGAGTTAAGGTGATGGCCAAATTGGATATCGCCGAAAAGAGGATTCCTCAAGATGGTAG
AATTTCATTGCGTATAGGAGGACACAATATTGATGTCAGGGTTTCAACTTTACCATCAAACCATGGAGAACGAGTGGTTT
TAAGGATATTGGATAAACAAGCTGCTCAACTGGATTTAAATCTTTTGGGTATGCCTAAACATACATTAAAAACAGTACGA
CAAATGATTGCTGAACCTCATGGCATTATTTTAGTCACTGGCCCCACAGGTTCGGGCAAGACAACCTCTCTCTATGCTAT
GCTCACTGAATTAAATCAGGTGACTCGTAATATTTTAACCATTGAAGACCCCATTGAATATGACTTGCCTGGTGTAGGAC
AAACTCAAGTTAATACTAAAGTTCAAATGACTTTTGCCAAAGGATTAAGGGCCATATTACGTCAAGATCCGGATGTTGTC
ATGATAGGAGAGATCCGTGATTTGGAAACGGCTGAAATTGCTGTTCAAGCCAGCTTAACAGGGCATTTGGTACTATCAAC
CCTTCATACCAATACTGCATTGGGAGCATTAACTCGCCTGCATGATATGGGAGTTGAGTCTTTTTTATTGTCATCCAGTA
TAGTCGGGCTCATTGCGCAACGTTTGGTAAGGAAATTATGCTCTCATTGCAAAACACCTCATGAATTAAGACAGGAAGAA
AAGGAGTTAATAGGTCTTAAACCTGATGCTGATGTATCTCAGGTCTTTGAACCCAAGGGATGCGACTCATGCAACCACTT
GGGTTATAAAGGCAGAACAGGAATTTATGAATTAATTATCGTAGATGAAACCCTGAGAGGAATGATTCATCGTAATGAAA
ATATTCAAACAATGGAAAATTATTTACGACCAACCACACCGAGCATACGTGAAGATGGTTTTAAAAGAGTATTATCTGGC
GATACGTCACTTGCAGAAATTTTAAGAGTAACATCCCAAAATTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (119-385)

3D structure

• AlphaFold DB: I7HJU3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Vibrio cholerae strain A1552	43.75	93.927	0.411
pilB	Vibrio campbellii strain DS40M4	46.798	82.186	0.385
pilB	Vibrio parahaemolyticus RIMD 2210633	45.792	81.781	0.374
pilB	Acinetobacter baumannii D1279779	44.471	84.211	0.374
pilB	Acinetobacter baylyi ADP1	47.301	78.745	0.372