Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   NST22_RS12540 Genome accession   NZ_CP150156
Coordinates   2445797..2446834 (-) Length   345 a.a.
NCBI ID   WP_009967727.1    Uniprot ID   A0AAE2SM96
Organism   Bacillus sp. PS237     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2440797..2451834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST22_RS12490 (NST22_12490) tasA 2440895..2441680 (-) 786 WP_003230183.1 biofilm matrix protein TasA -
  NST22_RS12495 (NST22_12495) - 2441744..2442316 (-) 573 WP_003230181.1 signal peptidase I -
  NST22_RS12500 (NST22_12500) tapA 2442300..2443061 (-) 762 WP_003230180.1 amyloid fiber anchoring/assembly protein TapA -
  NST22_RS12505 (NST22_12505) - 2443333..2443659 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  NST22_RS12510 (NST22_12510) - 2443701..2443880 (-) 180 WP_003230176.1 YqzE family protein -
  NST22_RS12515 (NST22_12515) comGG 2443951..2444325 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  NST22_RS12520 (NST22_12520) comGF 2444326..2444709 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  NST22_RS12525 (NST22_12525) comGE 2444735..2445082 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  NST22_RS12530 (NST22_12530) comGD 2445066..2445497 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  NST22_RS12535 (NST22_12535) comGC 2445487..2445783 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  NST22_RS12540 (NST22_12540) comGB 2445797..2446834 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  NST22_RS12545 (NST22_12545) comGA 2446821..2447891 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  NST22_RS12550 (NST22_12550) corA 2448303..2449256 (-) 954 WP_004399136.1 magnesium transporter CorA -
  NST22_RS12555 (NST22_12555) - 2449399..2450727 (+) 1329 WP_009967730.1 hemolysin family protein -
  NST22_RS12560 (NST22_12560) rsbRD 2450780..2451616 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39762.26 Da        Isoelectric Point: 9.6257

>NTDB_id=963753 NST22_RS12540 WP_009967727.1 2445797..2446834(-) (comGB) [Bacillus sp. PS237]
MKKIRKVWLLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSLFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=963753 NST22_RS12540 WP_009967727.1 2445797..2446834(-) (comGB) [Bacillus sp. PS237]
ATGAAAAAGATTAGAAAAGTCTGGTTGTTAAAGGATCAAGCCAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACACTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCAATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCTTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

100

93.623

0.936