Detailed information NTG0963754

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACJEHF_RS11130
• Genome accession: NZ_CP174233
• Coordinates: 2457138..2459315 (-)
• Length: 725 a.a.
• NCBI ID: WP_010948552.1
• Uniprot ID: Q5ZRL5
• Organism: Legionella pneumophila strain SPF601
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2452138..2464315

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJEHF_RS11105 (ACJEHF_11105)	-	2453902..2454708 (+)	807	WP_010948556.1	sulfite exporter TauE/SafE family protein	-
ACJEHF_RS11110 (ACJEHF_11110)	lgt	2454726..2455496 (+)	771	WP_010948555.1	prolipoprotein diacylglyceryl transferase	-
ACJEHF_RS11115 (ACJEHF_11115)	thyA	2455493..2456287 (+)	795	WP_010948554.1	thymidylate synthase	-
ACJEHF_RS11120 (ACJEHF_11120)	-	2456363..2456632 (+)	270	WP_016357044.1	hypothetical protein	-
ACJEHF_RS11125 (ACJEHF_11125)	-	2456749..2457141 (-)	393	WP_010948553.1	thioesterase family protein	-
ACJEHF_RS11130 (ACJEHF_11130)	priA	2457138..2459315 (-)	2178	WP_010948552.1	primosomal protein N'	Machinery gene
ACJEHF_RS11135 (ACJEHF_11135)	-	2459316..2459810 (-)	495	WP_010948551.1	6-carboxytetrahydropterin synthase	-
ACJEHF_RS11140 (ACJEHF_11140)	-	2459813..2460925 (-)	1113	WP_015443911.1	class I SAM-dependent methyltransferase	-
ACJEHF_RS11145 (ACJEHF_11145)	-	2461102..2461857 (+)	756	WP_015443912.1	pteridine reductase	-
ACJEHF_RS11150 (ACJEHF_11150)	lgt2	2461983..2463893 (+)	1911	WP_010948548.1	Dot/Icm type IV secretion system effector glucosyltransferase Lgt2/LegC8	-

Sequence

Protein

• Length: 725 a.a.
• Molecular weight: 81788.11 Da
• Isoelectric Point: 9.1746

>NTDB_id=963754 ACJEHF_RS11130 WP_010948552.1 2457138..2459315(-) (priA) [Legionella pneumophila strain SPF601]
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQARVGIVINKVCDQQTGHSLKKITSIIDEKPLVPEDLLELCL
WVGSYYQSPLSEVIPLVIPKKYRLGLPCSLPTEDFYQLNIPVEKAKTLISKKAKKQHELIDFLSRHQKGISKQTLKQHGY
NGTQIAALLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVDSIFEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQVLVLVPEIGLTPQLLSRFTARFNHPITVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNYKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVEQFLKATKDQIQAHPLTVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS

Nucleotide

• Length: 2178 bp

>NTDB_id=963754 ACJEHF_RS11130 WP_010948552.1 2457138..2459315(-) (priA) [Legionella pneumophila strain SPF601]
ATGAACGATATTTATCAGGTTTGTATCCCCCATACCAATCGAGATTGTTTTGATTACGAAGCCCTGGATTTAACTCCATG
CATTGGCGGAAGAGTATGGGTTCCTTTTCGCAATCAAGCCCGAGTGGGCATTGTAATCAATAAAGTTTGTGACCAGCAAA
CGGGCCATTCGTTAAAGAAAATCACTTCAATAATCGATGAGAAACCTCTCGTTCCTGAAGATCTCTTAGAGCTTTGTCTA
TGGGTAGGAAGCTATTATCAGTCACCATTATCTGAAGTGATTCCTTTGGTAATACCAAAAAAATATCGCCTGGGTTTACC
CTGCTCACTACCTACAGAAGATTTTTATCAATTGAATATACCTGTTGAAAAGGCGAAGACACTCATCTCGAAAAAAGCAA
AAAAACAACATGAGTTGATTGATTTTTTAAGCAGGCACCAGAAAGGGATTTCCAAACAGACCCTAAAACAACATGGCTAC
AACGGAACTCAAATTGCGGCACTACTTGCTTGTCAAGCAATTAACCTGTCACAGCAAGTTACCTTGCCAACCAAAATTCC
TGAAAAACTCAGTCCACCGCTAACACTCAATCCTGAACAAGCCGTTGCCGTAGATTCCATTTTCGAACACATCCATCATT
ACCAATGCTTTTTACTGCAAGGTGTTACCGGGAGTGGTAAGACAGAGGTTTATTTGCATGTCATTGCCAAAATATTGGAG
CAAGGCAAACAAGTATTGGTGCTGGTACCAGAAATCGGGTTAACCCCTCAGCTTTTATCTCGTTTTACAGCCCGATTTAA
CCACCCCATAACCGTATTACATTCCAATTTGAATGAATCAGAAAGGCAAATTGCCTGGCAGCTGGCAAAAGAGAACAAAG
TTAAAATGGTGATTGGAACTCGTTCTGCCGTTTTTACCCCTCTACCCAATCTTGGTTTAATTATCATCGATGAAGAGCAT
GACAGTTCGCTCAAACAAATGGAAGGGGTGCGTTATTCTGCGCGAGATACTGCTTTAATGCGTGCTCATCTTGCCAATAT
TCCTATCATTCTTGGTACAGCCACTCCCAGTCTGGAAACCATCCACAACTACAAACAAAAAAAATATAATTTATTACGCT
TAACGCATAAAGCTGCCTCCACTACACCGTTACACTATCAACTGATTGATTTACGATCGCAAATGGTACAGCATGGCTTG
GCTCTCCCAACCCTTAAAATCATAGAAAATCATTTGCTGCAACAAAACCAGGTTCTTGTTTTCATTAACCGACGCGGCTT
TGCCCCAGTGTTACTTTGCCATCAATGTGGATGGATGGTTGATTGCAAAGCATGCGATAGCCATTTAACCTTGCATAAAC
AGGCAGGACAAATGATTTGTCATCATTGTGGATTAACCCAAAGAATACCCTTGTTTTGCAATAACTGCCAAAGTAAGGAA
TTAATCCCCGTAGGATCTGGCACCCAGAGAATTTATGAGTTTTTAAGCCATCAGTTTCCAGATACGAATGTTTTACGAAT
TGACCGAGATGAGGTTCGTAAAAAAAATTCTCTAAACAGCCATTTGGACAAAATTAATAAAGGGGAAGCTCAGCTGATCA
TAGGCACTCAAATGCTAGCCAAGGGACACCATTTCCCCCGTCTATCCCTGGTCGTTGTCGTAGATGCTGATGCAGGCTTT
TACAACCAGGATTTCCGGGCTATTGAACATTTGGGGCAATTATTAACTCAAGTATCTGGACGAGCAGGGCGAGCAGAACA
TCCCGGGCAAGTATTGATTCAAACTTATTTACCTGATCATCCCCTGCTTAATCTACTCATTCAGCATGGGTATGATGAAT
TTGCGAACGCTTTATTAATTTCAAGACAAGAGGCTGAAATGCCACCTTTTCAGTTTTTAGCGGTCATCAGAGCTCAGGGC
AAAACCGTCAACAAGGTGGAGCAATTCCTAAAAGCAACCAAAGATCAAATTCAAGCTCATCCTCTCACGGTACTGGGTCC
TGCACCAGCTCCTTTACCACGAAAAGCTAACCAGCATAGGATGCAACTATTAATTAAATCCCCTTCAAGAAAAGTTCTAA
AAAGTTCATTGACGCAATTAAGAGAGTGGTTAACAATGAATAAATTAAGTAATGGCATCCGCTGGAATGTGGATGTTGAT
CCTATGGACTTATCATGA

Domains

Predicted by InterProScan.

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (442-468)

• Helicase conserved C-terminal domain (Helicase_C) (489-585)

• Primosomal protein N C-terminal domain (PriA_C) (629-721)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-99)

• Type III restriction enzyme, res subunit (ResIII) (195-357)

3D structure

• AlphaFold DB: Q5ZRL5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	42.219	100	0.43