Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NST22_RS12545 Genome accession   NZ_CP150156
Coordinates   2446821..2447891 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus sp. PS237     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2441821..2452891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST22_RS12500 (NST22_12500) tapA 2442300..2443061 (-) 762 WP_003230180.1 amyloid fiber anchoring/assembly protein TapA -
  NST22_RS12505 (NST22_12505) - 2443333..2443659 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  NST22_RS12510 (NST22_12510) - 2443701..2443880 (-) 180 WP_003230176.1 YqzE family protein -
  NST22_RS12515 (NST22_12515) comGG 2443951..2444325 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  NST22_RS12520 (NST22_12520) comGF 2444326..2444709 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  NST22_RS12525 (NST22_12525) comGE 2444735..2445082 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  NST22_RS12530 (NST22_12530) comGD 2445066..2445497 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  NST22_RS12535 (NST22_12535) comGC 2445487..2445783 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  NST22_RS12540 (NST22_12540) comGB 2445797..2446834 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  NST22_RS12545 (NST22_12545) comGA 2446821..2447891 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  NST22_RS12550 (NST22_12550) corA 2448303..2449256 (-) 954 WP_004399136.1 magnesium transporter CorA -
  NST22_RS12555 (NST22_12555) - 2449399..2450727 (+) 1329 WP_009967730.1 hemolysin family protein -
  NST22_RS12560 (NST22_12560) rsbRD 2450780..2451616 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -
  NST22_RS12570 (NST22_12570) mgsR 2451840..2452220 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  NST22_RS12575 (NST22_12575) - 2452452..2452697 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=963754 NST22_RS12545 WP_004399124.1 2446821..2447891(-) (comGA) [Bacillus sp. PS237]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=963754 NST22_RS12545 WP_004399124.1 2446821..2447891(-) (comGA) [Bacillus sp. PS237]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGTTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1