Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WMC18_RS01780 Genome accession   NZ_CP150130
Coordinates   329818..330255 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain SEC-482     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 324818..335255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WMC18_RS01740 (WMC18_01740) - 325397..325561 (-) 165 WP_370648487.1 molybdopterin dinucleotide binding domain-containing protein -
  WMC18_RS01745 (WMC18_01745) - 325675..326877 (+) 1203 WP_014304313.1 GTP-binding protein -
  WMC18_RS01750 (WMC18_01750) - 326942..328078 (+) 1137 WP_107994550.1 zinc-dependent alcohol dehydrogenase -
  WMC18_RS01755 (WMC18_01755) - 328093..328527 (+) 435 WP_003156592.1 RDD family protein -
  WMC18_RS01760 (WMC18_01760) - 328645..328920 (+) 276 WP_369719109.1 YckD family protein -
  WMC18_RS01765 (WMC18_01765) - 329024..329140 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  WMC18_RS01770 (WMC18_01770) - 329139..329357 (+) 219 Protein_308 family 1 glycosylhydrolase -
  WMC18_RS01775 (WMC18_01775) nin/comJ 329398..329796 (-) 399 WP_003156589.1 competence protein ComJ Regulator
  WMC18_RS01780 (WMC18_01780) nucA/comI 329818..330255 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  WMC18_RS01785 (WMC18_01785) hxlB 330614..331171 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  WMC18_RS01790 (WMC18_01790) hxlA 331168..331803 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  WMC18_RS01795 (WMC18_01795) - 332035..332397 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=963021 WMC18_RS01780 WP_003156588.1 329818..330255(-) (nucA/comI) [Bacillus velezensis strain SEC-482]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=963021 WMC18_RS01780 WP_003156588.1 329818..330255(-) (nucA/comI) [Bacillus velezensis strain SEC-482]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGTCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641