Detailed information NTG0963007

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: WMN84_RS20615
• Genome accession: NZ_CP150128
• Coordinates: 4257870..4258670 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 2024CK-00187
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4252870..4263670

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WMN84_RS20580 (WMN84_20570)	gspH	4253026..4253541 (+)	516	WP_004213437.1	type II secretion system minor pseudopilin GspH	-
WMN84_RS20585 (WMN84_20575)	gspI	4253538..4253903 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
WMN84_RS20590 (WMN84_20580)	gspJ	4253900..4254496 (+)	597	WP_004222700.1	type II secretion system minor pseudopilin GspJ	-
WMN84_RS20595 (WMN84_20585)	gspK	4254493..4255479 (+)	987	WP_004222697.1	type II secretion system minor pseudopilin GspK	-
WMN84_RS20600 (WMN84_20590)	gspL	4255466..4256659 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
WMN84_RS20605 (WMN84_20595)	-	4256652..4257137 (+)	486	WP_004151943.1	type II secretion system protein M	-
WMN84_RS20610 (WMN84_20600)	-	4257127..4257873 (+)	747	WP_004145897.1	type II secretion system protein N	-
WMN84_RS20615 (WMN84_20605)	pilD	4257870..4258670 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
WMN84_RS20620 (WMN84_20610)	hrpB	4258718..4261147 (-)	2430	WP_020317204.1	ATP-dependent helicase HrpB	-
WMN84_RS20625 (WMN84_20615)	thpR	4261220..4261756 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
WMN84_RS20630 (WMN84_20620)	sfsA	4261756..4262472 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
WMN84_RS20635 (WMN84_20625)	dksA	4262635..4263090 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=963007 WMN84_RS20615 WP_002888947.1 4257870..4258670(+) (pilD) [Klebsiella pneumoniae strain 2024CK-00187]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=963007 WMN84_RS20615 WP_002888947.1 4257870..4258670(+) (pilD) [Klebsiella pneumoniae strain 2024CK-00187]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425