Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   WKU35_RS07515 Genome accession   NZ_CP150127
Coordinates   1566796..1567482 (-) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus subsp. aureus RN4220     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1561796..1572482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKU35_RS07495 rpsT 1562710..1562961 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  WKU35_RS07500 holA 1563006..1563980 (-) 975 WP_001282562.1 DNA polymerase III subunit delta -
  WKU35_RS07505 comEC 1564037..1566238 (-) 2202 WP_001805151.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  WKU35_RS07510 - 1566243..1566704 (-) 462 WP_000439693.1 ComE operon protein 2 -
  WKU35_RS07515 comEA 1566796..1567482 (-) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  WKU35_RS07520 - 1567522..1568238 (-) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  WKU35_RS07525 rsfS 1568241..1568594 (-) 354 WP_001088022.1 ribosome silencing factor -
  WKU35_RS07530 yqeK 1568595..1569179 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  WKU35_RS07535 nadD 1569169..1569738 (-) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  WKU35_RS07540 yhbY 1569741..1570031 (-) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  WKU35_RS07545 aroE 1570035..1570841 (-) 807 WP_000666761.1 shikimate dehydrogenase -
  WKU35_RS07550 yqeH 1570855..1571955 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=962974 WKU35_RS07515 WP_001792568.1 1566796..1567482(-) (comEA) [Staphylococcus aureus subsp. aureus RN4220]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=962974 WKU35_RS07515 WP_001792568.1 1566796..1567482(-) (comEA) [Staphylococcus aureus subsp. aureus RN4220]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969