Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   WKU35_RS07265 Genome accession   NZ_CP150127
Coordinates   1519428..1519925 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus subsp. aureus RN4220     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1514428..1524925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKU35_RS07240 gcvPB 1514590..1516062 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  WKU35_RS07245 gcvPA 1516055..1517401 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  WKU35_RS07250 gcvT 1517421..1518512 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  WKU35_RS07255 - 1518671..1519195 (-) 525 WP_001015117.1 shikimate kinase -
  WKU35_RS07260 - 1519185..1519331 (-) 147 WP_001789879.1 hypothetical protein -
  WKU35_RS07265 comGF 1519428..1519925 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  WKU35_RS07270 comGE 1519843..1520142 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  WKU35_RS07275 comGD 1520129..1520575 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  WKU35_RS07280 comGC 1520553..1520864 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  WKU35_RS07285 comGB 1520878..1521948 (-) 1071 Protein_1428 competence type IV pilus assembly protein ComGB -
  WKU35_RS07290 comGA 1521920..1522894 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  WKU35_RS07295 - 1522946..1523569 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  WKU35_RS07300 - 1523566..1523895 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  WKU35_RS07305 - 1523895..1524881 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=962967 WKU35_RS07265 WP_001789864.1 1519428..1519925(-) (comGF) [Staphylococcus aureus subsp. aureus RN4220]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=962967 WKU35_RS07265 WP_001789864.1 1519428..1519925(-) (comGF) [Staphylococcus aureus subsp. aureus RN4220]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964