Detailed information    

insolico Bioinformatically predicted

Overview

Name   sigH   Type   Regulator
Locus tag   WKU35_RS02495 Genome accession   NZ_CP150127
Coordinates   526687..527256 (+) Length   189 a.a.
NCBI ID   WP_000872873.1    Uniprot ID   -
Organism   Staphylococcus aureus subsp. aureus RN4220     
Function   promote expression of competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 521687..532256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKU35_RS02470 cysE 522900..523547 (+) 648 WP_001805237.1 serine O-acetyltransferase -
  WKU35_RS02475 cysS 523531..524931 (+) 1401 WP_000631963.1 cysteine--tRNA ligase -
  WKU35_RS02480 - 524924..525328 (+) 405 WP_000370182.1 Mini-ribonuclease 3 -
  WKU35_RS02485 rlmB 525336..526082 (+) 747 WP_000390328.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  WKU35_RS02490 - 526082..526606 (+) 525 WP_000664738.1 NYN domain-containing protein -
  WKU35_RS02495 sigH 526687..527256 (+) 570 WP_000872873.1 sigma-70 family RNA polymerase sigma factor Regulator
  WKU35_RS02500 rpmG 527371..527514 (+) 144 WP_001788193.1 50S ribosomal protein L33 -
  WKU35_RS02505 secE 527570..527752 (+) 183 WP_001074473.1 preprotein translocase subunit SecE -
  WKU35_RS02510 nusG 527765..528313 (+) 549 WP_001288302.1 transcription termination/antitermination protein NusG -
  WKU35_RS02515 rplK 528494..528916 (+) 423 WP_001085792.1 50S ribosomal protein L11 -
  WKU35_RS02520 rplA 529124..529816 (+) 693 WP_001074619.1 50S ribosomal protein L1 -
  WKU35_RS02525 rplJ 530088..530588 (+) 501 WP_001273085.1 50S ribosomal protein L10 -
  WKU35_RS02530 rplL 530631..530999 (+) 369 WP_001273586.1 50S ribosomal protein L7/L12 -
  WKU35_RS02535 - 531174..531782 (+) 609 WP_000020304.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 189 a.a.        Molecular weight: 23028.91 Da        Isoelectric Point: 9.4170

>NTDB_id=962955 WKU35_RS02495 WP_000872873.1 526687..527256(+) (sigH) [Staphylococcus aureus subsp. aureus RN4220]
MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFNHYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQC
LITSVKYDYLRKYLATNKRMDNLINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
AQLMHVKEKVIYNAIQRCKNKIKRYFKMI

Nucleotide


Download         Length: 570 bp        

>NTDB_id=962955 WKU35_RS02495 WP_000872873.1 526687..527256(+) (sigH) [Staphylococcus aureus subsp. aureus RN4220]
TTGAAATACGATTTGACAACTCAAGACAGTACAATCAAACGTAACAATGCAATAAATGATAAAGACTTCGAAAAGTTAGT
AATGGATCTACAACCATTAATTATTCGACGCATCAAAACATTTGGATTTAATCATTATGATTTAGAAGACTTATATCAAG
AAATACTTATACGGATGTATAGGTCGGTCCAAACATTTGATTTTAGTGGAGAGCAGCCTTTCACAAATTATGTTCAATGT
TTAATTACGTCTGTAAAGTATGATTATTTGAGAAAATATTTAGCTACAAATAAAAGAATGGATAATTTGATTAATGAATA
TAGAGTTACGTATCCATGTGCAATAAAGCGTTTTGATGTTGAAAACAATTATTTGAATAAATTAGCAATTAAAGAGTTGA
TTTGTCAGTTTAAGTATTTGAGTGCATTTGAAAAAGATGTCATGTATTTAATGTGTGAACAATATAAGCCGAGAGAAATT
GCTCAATTGATGCATGTAAAAGAGAAAGTGATTTATAATGCCATACAACGATGTAAAAATAAAATAAAACGTTATTTCAA
AATGATTTGA

Domains

Predicted by InterproScan.

Sigma-70 region 2 (Sigma70_r2)

(26-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sigH Staphylococcus aureus N315

99.471

100

0.995
  sigH Staphylococcus aureus MW2

98.413

100

0.984