Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCX50_RS08320 Genome accession   NZ_AP026403
Coordinates   1713287..1714423 (-) Length   378 a.a.
NCBI ID   WP_223925077.1    Uniprot ID   -
Organism   Aeromonas caviae strain KAM643     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1708287..1719423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCX50_RS08295 (KAM643c_15930) - 1709182..1709739 (-) 558 WP_010675206.1 TMEM165/GDT1 family protein -
  OCX50_RS08300 (KAM643c_15940) - 1709993..1710322 (-) 330 WP_010675205.1 translation initiation factor -
  OCX50_RS08305 (KAM643c_15950) - 1710442..1710669 (+) 228 WP_010675204.1 FeoA family protein -
  OCX50_RS08310 (KAM643c_15960) feoB 1710666..1712936 (+) 2271 WP_010675203.1 Fe(2+) transporter permease subunit FeoB -
  OCX50_RS08315 (KAM643c_15970) - 1712983..1713225 (+) 243 WP_010675202.1 FeoC-like transcriptional regulator -
  OCX50_RS08320 (KAM643c_15980) pilU 1713287..1714423 (-) 1137 WP_223925077.1 type IVa pilus ATPase TapW Machinery gene
  OCX50_RS08325 (KAM643c_15990) - 1714480..1714836 (-) 357 WP_010675200.1 DMT family protein -
  OCX50_RS08330 (KAM643c_16000) - 1714949..1715680 (+) 732 WP_128345494.1 ABC transporter substrate-binding protein -
  OCX50_RS08335 (KAM643c_16010) btuC 1715756..1716763 (+) 1008 WP_124244908.1 vitamin B12 ABC transporter permease BtuC -
  OCX50_RS08340 (KAM643c_16020) - 1716750..1717523 (+) 774 WP_010675197.1 ATP-binding cassette domain-containing protein -
  OCX50_RS08345 (KAM643c_16030) - 1717731..1718795 (-) 1065 WP_223925073.1 3-deoxy-7-phosphoheptulonate synthase -
  OCX50_RS08350 (KAM643c_16040) - 1718937..1719317 (+) 381 WP_052816484.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42275.33 Da        Isoelectric Point: 6.4280

>NTDB_id=96230 OCX50_RS08320 WP_223925077.1 1713287..1714423(-) (pilU) [Aeromonas caviae strain KAM643]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLTQEPLGPGEVARIAYDIMDLEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHQKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGERRAAVEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDEGDLF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=96230 OCX50_RS08320 WP_223925077.1 1713287..1714423(-) (pilU) [Aeromonas caviae strain KAM643]
ATGGAATTGAGAGACATGCTGCAGATCCTGGCCAAACAGGACGGTTCCGATCTCTATCTCTCCACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGCCTTGCGCCCCCTCACCCAGGAGCCCCTGGGGCCGGGCGAGGTGGCCCGCATCGCCTACGACA
TCATGGATCTCGAGCAGAAACAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAGCAGCGCAATGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAACTCCCTCCGGTGCTCCTCGACACCATCATGGAGAAGCGGGGTCTGGTGCTCTTCGTCGGCGGCACGGGGT
CCGGCAAATCCACATCGCTCGCGGCCCTCATCGATCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCAGAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGCCAGGCACCGGACGTCATCCTCATCGGCGAAATCCGCGATCGGGAGACCATGGAGCATG
CCCTCGCCTTCTCGGAAACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCAGACCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGAGCGCCGGGCGGCGGTGGAGGTGATGCTCGGAACCCACACGGTGCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAAAAATCCAGGGCCCTTGGCATGGTGACCTTCGACAGCACTCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCGGACTCCCAGAACAACCTGCGCCTCAA
GATCAAACTCTGGCGGGAGAAGGGCCAGATTGGCAACAGCGCGGCGGCGAGCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGGGCGACCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

99.206

0.537
  pilU Acinetobacter baylyi ADP1

52.514

94.709

0.497
  pilU Vibrio cholerae strain A1552

49.858

93.386

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.183

89.683

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.171

92.857

0.373
  pilT Vibrio cholerae strain A1552

40.171

92.857

0.373
  pilT Pseudomonas aeruginosa PAK

40.698

91.005

0.37
  pilT Legionella pneumophila strain ERS1305867

41.916

88.36

0.37
  pilT Legionella pneumophila strain Lp02

41.916

88.36

0.37
  pilT Pseudomonas stutzeri DSM 10701

40.407

91.005

0.368
  pilT Acinetobacter baumannii strain A118

41.018

88.36

0.362
  pilT Acinetobacter nosocomialis M2

41.018

88.36

0.362
  pilT Acinetobacter baumannii D1279779

41.018

88.36

0.362

Multiple sequence alignment