Detailed information NTG0959499

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: WJ058_RS21515
• Genome accession: NZ_CP149491
• Coordinates: 4453937..4454737 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola strain AJ292
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4448937..4459737

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WJ058_RS21480 (WJ058_21450)	gspH	4449090..4449605 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
WJ058_RS21485 (WJ058_21455)	gspI	4449602..4449967 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
WJ058_RS21490 (WJ058_21460)	gspJ	4449964..4450560 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
WJ058_RS21495 (WJ058_21465)	gspK	4450557..4451537 (+)	981	WP_077263807.1	type II secretion system minor pseudopilin GspK	-
WJ058_RS21500 (WJ058_21470)	gspL	4451530..4452726 (+)	1197	WP_117107499.1	type II secretion system protein GspL	-
WJ058_RS21505 (WJ058_21475)	-	4452719..4453204 (+)	486	WP_012968921.1	type II secretion system protein M	-
WJ058_RS21510 (WJ058_21480)	-	4453194..4453940 (+)	747	WP_023322077.1	type II secretion system protein N	-
WJ058_RS21515 (WJ058_21485)	pilD	4453937..4454737 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
WJ058_RS21520 (WJ058_21490)	hrpB	4454784..4457213 (-)	2430	WP_096834294.1	ATP-dependent helicase HrpB	-
WJ058_RS21525 (WJ058_21495)	thpR	4457286..4457822 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
WJ058_RS21530 (WJ058_21500)	sfsA	4457822..4458538 (+)	717	WP_022066362.1	DNA/RNA nuclease SfsA	-
WJ058_RS21535 (WJ058_21505)	dksA	4458701..4459156 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=959499 WJ058_RS21515 WP_008807384.1 4453937..4454737(+) (pilD) [Klebsiella variicola strain AJ292]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=959499 WJ058_RS21515 WP_008807384.1 4453937..4454737(+) (pilD) [Klebsiella variicola strain AJ292]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425