Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   WJ062_RS06295 Genome accession   NZ_CP149486
Coordinates   1283813..1285138 (-) Length   441 a.a.
NCBI ID   WP_038434193.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain EMM113     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1278813..1290138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ062_RS06285 (WJ062_06255) hpf 1282548..1283096 (-) 549 WP_406848696.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WJ062_RS06290 (WJ062_06260) - 1283176..1283841 (-) 666 WP_002983685.1 ComF family protein -
  WJ062_RS06295 (WJ062_06265) comFA/cflA 1283813..1285138 (-) 1326 WP_038434193.1 DEAD/DEAH box helicase Machinery gene
  WJ062_RS06300 (WJ062_06270) - 1285193..1285825 (+) 633 WP_002988970.1 YigZ family protein -
  WJ062_RS06305 (WJ062_06275) cysK 1285953..1286894 (+) 942 WP_011285046.1 cysteine synthase A -
  WJ062_RS06310 (WJ062_06280) - 1286912..1287289 (-) 378 WP_002991882.1 S1 RNA-binding domain-containing protein -
  WJ062_RS06315 (WJ062_06285) - 1287289..1288689 (-) 1401 WP_011054832.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  WJ062_RS06320 (WJ062_06290) - 1288726..1289367 (-) 642 WP_002983671.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49819.31 Da        Isoelectric Point: 10.0009

>NTDB_id=959443 WJ062_RS06295 WP_038434193.1 1283813..1285138(-) (comFA/cflA) [Streptococcus pyogenes strain EMM113]
MESIENSYGRLLLESQLPDSAKQLAQPLKSVVISRGKMICQRCHYQLDEEARLPNGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWSGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=959443 WJ062_RS06295 WP_038434193.1 1283813..1285138(-) (comFA/cflA) [Streptococcus pyogenes strain EMM113]
ATGGAGTCTATTGAAAATAGTTATGGACGATTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAAGCAGCTTGCTCAACC
TTTAAAAAGTGTAGTAATATCGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAATGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGAGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCGAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(98-244)

Helicase conserved C-terminal domain (Helicase_C)

(289-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae D39

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae R6

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae TIGR4

54.177

95.011

0.515
  comFA/cflA Streptococcus mitis NCTC 12261

52.844

95.692

0.506
  comFA/cflA Streptococcus mitis SK321

51.896

95.692

0.497
  comFA Lactococcus lactis subsp. cremoris KW2

47.368

90.476

0.429
  comFA Latilactobacillus sakei subsp. sakei 23K

39.851

91.61

0.365