Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACIRXL_RS03480 Genome accession   NZ_CP173236
Coordinates   715010..715552 (-) Length   180 a.a.
NCBI ID   WP_410679751.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain npAP/GA-USA/20231216/S1-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 710010..720552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIRXL_RS03460 (ACIRXL_03460) - 711108..711314 (+) 207 WP_410679755.1 hypothetical protein -
  ACIRXL_RS03465 (ACIRXL_03465) pilA 711511..713004 (-) 1494 WP_410679754.1 signal recognition particle-docking protein FtsY Machinery gene
  ACIRXL_RS03470 (ACIRXL_03470) rsmD 713160..713768 (+) 609 WP_410679753.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACIRXL_RS03475 (ACIRXL_03475) dprA 713765..714865 (-) 1101 WP_410679752.1 DNA-processing protein DprA Machinery gene
  ACIRXL_RS03480 (ACIRXL_03480) pilF/pilF2 715010..715552 (-) 543 WP_410679751.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACIRXL_RS03485 (ACIRXL_03485) - 715586..716755 (-) 1170 WP_264256081.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACIRXL_RS03490 (ACIRXL_03490) - 717127..718179 (-) 1053 WP_264256080.1 AI-2E family transporter -
  ACIRXL_RS03495 (ACIRXL_03495) glnB 718182..718520 (-) 339 WP_017805299.1 nitrogen regulatory protein P-II -
  ACIRXL_RS03500 (ACIRXL_03500) mog 718549..719127 (-) 579 WP_264256079.1 molybdopterin adenylyltransferase -
  ACIRXL_RS03505 (ACIRXL_03505) - 719146..720075 (-) 930 WP_410679750.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20701.70 Da        Isoelectric Point: 8.0752

>NTDB_id=957759 ACIRXL_RS03480 WP_410679751.1 715010..715552(-) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/GA-USA/20231216/S1-1]
MKIYQCILSILFSLFLVACVSTPLNKQDFDKQQAAKARVELGIGYLEQNELGLAKQNLDKALAYAPNYYLVHSALAYFYQ
LQGENTRAENAYLKALKLDDKQGDVFNNYAVFLCKQGQFEQAYLRFEQALATPNYYQQADTYENIVLCALAEKNAIRYEK
YKGLLDKLDPKRNQKTNVIH

Nucleotide


Download         Length: 543 bp        

>NTDB_id=957759 ACIRXL_RS03480 WP_410679751.1 715010..715552(-) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/GA-USA/20231216/S1-1]
ATGAAAATTTATCAATGTATCTTAAGCATTCTTTTTTCTCTCTTTCTCGTAGCCTGTGTTTCCACTCCCTTAAATAAGCA
AGACTTTGATAAGCAGCAAGCCGCAAAGGCGAGAGTGGAACTTGGCATTGGCTATCTTGAGCAAAATGAACTTGGTCTGG
CGAAGCAAAATTTAGACAAAGCTTTAGCTTATGCCCCGAATTATTATTTAGTGCATTCAGCATTGGCATATTTTTATCAG
CTTCAAGGGGAGAATACTCGTGCGGAAAATGCGTATTTGAAAGCCCTAAAACTCGACGATAAACAAGGCGATGTATTTAA
TAATTATGCCGTCTTTTTGTGCAAACAAGGACAATTCGAACAGGCTTATCTACGATTTGAACAAGCTCTCGCCACACCTA
ATTATTATCAGCAAGCGGATACTTACGAGAATATTGTACTTTGTGCGTTAGCGGAGAAAAATGCAATACGTTATGAAAAA
TATAAAGGCTTGCTGGATAAACTAGATCCTAAAAGAAATCAAAAGACAAATGTTATTCATTAA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

49.153

98.333

0.483
  pilF Glaesserella parasuis strain SC1401

42.857

100

0.433