Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACIO07_RS01240 Genome accession   NZ_CP173228
Coordinates   224984..225526 (-) Length   180 a.a.
NCBI ID   WP_017805302.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain npAP/GA-USA/20231220/S2-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 219984..230526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIO07_RS01225 (ACIO07_01225) pilA 221485..222978 (-) 1494 WP_410682704.1 signal recognition particle-docking protein FtsY Machinery gene
  ACIO07_RS01230 (ACIO07_01230) rsmD 223134..223742 (+) 609 WP_264245187.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACIO07_RS01235 (ACIO07_01235) dprA 223739..224839 (-) 1101 WP_410682706.1 DNA-processing protein DprA Machinery gene
  ACIO07_RS01240 (ACIO07_01240) pilF/pilF2 224984..225526 (-) 543 WP_017805302.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACIO07_RS01245 (ACIO07_01245) - 225560..226720 (-) 1161 WP_410682708.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACIO07_RS01250 (ACIO07_01250) - 227173..228225 (-) 1053 WP_410682710.1 AI-2E family transporter -
  ACIO07_RS01255 (ACIO07_01255) glnB 228228..228566 (-) 339 WP_410682711.1 nitrogen regulatory protein P-II -
  ACIO07_RS01260 (ACIO07_01260) mog 228607..229185 (-) 579 WP_410682712.1 molybdopterin adenylyltransferase -
  ACIO07_RS01265 (ACIO07_01265) - 229204..230133 (-) 930 WP_115249942.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20715.73 Da        Isoelectric Point: 8.0752

>NTDB_id=957382 ACIO07_RS01240 WP_017805302.1 224984..225526(-) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/GA-USA/20231220/S2-3]
MKIYQCILSILFSLFLVACVSTPLNKQDFDKQQAAKARVELGIGYLEQNELGLAKQNLDKALAYAPNYYLVHSALAYFYQ
LQGENTRAENAYLKALKLDDKQGDVFNNYAVFLCKQGQFEQAYLRFEQALATPNYYQQADTYENIVLCALAEKNAIRYEK
YKGLLEKLDPKRNQKTNVIH

Nucleotide


Download         Length: 543 bp        

>NTDB_id=957382 ACIO07_RS01240 WP_017805302.1 224984..225526(-) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/GA-USA/20231220/S2-3]
ATGAAAATTTATCAATGTATCTTAAGCATTCTTTTTTCTCTCTTTCTCGTAGCCTGTGTTTCCACTCCCTTAAATAAGCA
AGACTTTGATAAGCAGCAAGCCGCAAAGGCGAGAGTGGAACTTGGCATTGGCTATCTTGAGCAAAATGAACTTGGGCTGG
CGAAGCAAAATTTAGACAAAGCCTTAGCTTATGCCCCGAATTATTATTTAGTGCATTCAGCGTTGGCATATTTTTATCAG
CTTCAAGGGGAGAATACTCGTGCGGAAAATGCGTATTTGAAAGCCCTAAAACTCGACGATAAACAAGGCGATGTATTTAA
TAATTATGCCGTTTTTTTGTGCAAACAAGGACAATTCGAACAGGCTTATCTACGATTTGAACAAGCTCTCGCCACACCTA
ATTATTATCAGCAAGCGGATACTTACGAGAATATTGTACTTTGTGCGTTAGCGGAGAAAAATGCAATACGTTATGAAAAA
TATAAAGGCTTGCTGGAGAAACTAGATCCTAAAAGAAATCAAAAGACAAATGTTATTCATTAA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

50.292

95

0.478
  pilF Glaesserella parasuis strain SC1401

43.407

100

0.439