Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AM10699_RS16480 Genome accession   NZ_AP026075
Coordinates   3696792..3698798 (-) Length   668 a.a.
NCBI ID   WP_012161083.1    Uniprot ID   B0CAY6
Organism   Acaryochloris marina MBIC10699     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3691792..3703798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM10699_RS16455 (AM10699_32270) - 3692144..3692896 (+) 753 WP_010473380.1 isoprenyl transferase -
  AM10699_RS16460 (AM10699_32280) - 3693067..3693348 (-) 282 WP_010473382.1 DUF3143 domain-containing protein -
  AM10699_RS16465 (AM10699_32290) - 3693686..3694123 (-) 438 WP_148215651.1 J domain-containing protein -
  AM10699_RS16470 (AM10699_32300) - 3694314..3695534 (-) 1221 WP_010473386.1 type II secretion system F family protein -
  AM10699_RS16475 (AM10699_32310) pilT 3695592..3696713 (-) 1122 WP_012161082.1 type IV pilus twitching motility protein PilT Machinery gene
  AM10699_RS16480 (AM10699_32320) pilB/pilB1 3696792..3698798 (-) 2007 WP_012161083.1 GspE/PulE family protein Machinery gene
  AM10699_RS16485 (AM10699_32330) grpE 3699419..3700207 (+) 789 WP_012161085.1 nucleotide exchange factor GrpE -
  AM10699_RS16490 (AM10699_32340) dnaK 3700371..3702359 (+) 1989 WP_261890617.1 molecular chaperone DnaK -
  AM10699_RS16495 (AM10699_32350) dnaJ 3702409..3703533 (+) 1125 WP_012161087.1 molecular chaperone DnaJ -
  AM10699_RS16500 (AM10699_32360) - 3703530..3703790 (+) 261 WP_041658726.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 668 a.a.        Molecular weight: 74237.74 Da        Isoelectric Point: 4.6893

>NTDB_id=95691 AM10699_RS16480 WP_012161083.1 3696792..3698798(-) (pilB/pilB1) [Acaryochloris marina MBIC10699]
MVNSSSQRRALVTRHNFSPFGNKLISTGYVDQDQMREATLESRRSGRSLVEILQGLTGQSLPPELLRQYKRQQLFELKIL
YGIDSLDLELSQIPTDQIEELIQNYISLDLCRRYQLVPLTLDKETDPATLLVAMVDPDNLGALDDLGRILREKNLTLQRM
VIAVEDYQQLISKYLDQQAAEQKANEKPIEFDLDDDLDAIAGLDDLPSDELETDLGAALQDAETAPIIGLVNKILAKALQ
NGVSDIHIEPQEDHLRVRFRKDGVLQQAFDPLPRKIIPAVISRFKIISDLDIAERRLPQDGRIRRLFQGRKIDFRVNTLP
SRYGEKIVLRILDNSSTQLGLDKLVSDEDSLNIVREMVSRPFGLILVTGPTGSGKTTTLYSALAECNHPGVNISTAEDPI
EYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLAEMEV
EPFMVSASLLGVVAQRLVRRVCSDCKVPYQPTPTELGRFGLSASPGTEITFYKANTLSADELQTAQATGQTVCSTCNGIG
YKGRCGVYEVMQITETLQTLITEGAPTERIKEVAVEEGMKTLLAYSLELVKKGDTTLAEVERVTFTDSGLEAELKAKRKT
SLTCRVCDADLRPEWLDCPYCTTPRFQD

Nucleotide


Download         Length: 2007 bp        

>NTDB_id=95691 AM10699_RS16480 WP_012161083.1 3696792..3698798(-) (pilB/pilB1) [Acaryochloris marina MBIC10699]
ATGGTTAACTCTTCCTCACAGCGGCGTGCTCTCGTTACTCGGCACAATTTTTCCCCTTTTGGCAATAAGCTGATTTCAAC
AGGTTATGTTGACCAAGACCAAATGCGGGAAGCGACCCTAGAAAGTCGACGCTCAGGACGTTCACTTGTCGAAATTCTTC
AAGGACTCACTGGACAGTCTTTACCACCAGAACTTTTACGTCAATATAAACGACAACAACTTTTTGAACTAAAAATACTG
TATGGCATCGACTCTTTAGATCTTGAGTTAAGCCAAATTCCAACGGATCAAATCGAAGAATTGATCCAGAATTATATTTC
TCTTGACCTCTGTCGACGCTATCAGCTGGTTCCCCTAACCCTTGATAAAGAAACGGATCCAGCAACATTGCTGGTGGCAA
TGGTTGATCCAGATAATTTAGGCGCTCTGGACGATCTAGGGCGTATTCTTAGAGAGAAAAATCTAACCTTGCAACGCATG
GTGATTGCGGTTGAGGACTATCAGCAACTTATTAGTAAGTATCTCGATCAGCAAGCGGCTGAGCAAAAAGCAAATGAAAA
GCCGATCGAGTTCGATTTAGATGATGACTTAGATGCGATCGCAGGTTTAGATGATCTCCCTAGTGACGAACTTGAAACGG
ACTTGGGGGCGGCTCTTCAAGATGCGGAAACAGCTCCCATCATTGGTCTAGTTAACAAAATTCTGGCTAAAGCTTTACAG
AACGGTGTCTCTGATATTCATATTGAACCCCAAGAAGATCACCTTCGTGTTCGATTCCGTAAAGACGGTGTACTCCAACA
GGCCTTTGACCCATTGCCAAGAAAGATCATTCCTGCGGTTATTTCCCGTTTCAAAATCATTTCAGATCTTGATATTGCCG
AGCGCCGCCTTCCGCAAGATGGTCGTATCCGGCGACTTTTTCAAGGGCGCAAAATTGACTTTCGAGTGAATACCTTGCCG
AGCCGATATGGAGAGAAGATTGTACTTCGTATTCTGGATAACTCCTCGACTCAACTGGGATTAGATAAACTTGTTTCAGA
TGAAGATAGTCTAAACATTGTCAGAGAAATGGTCAGTAGACCGTTTGGACTAATTTTGGTGACAGGACCGACTGGTTCTG
GTAAAACAACGACTCTCTATTCTGCCTTAGCCGAATGTAATCACCCAGGCGTCAACATCAGCACTGCTGAGGACCCGATT
GAATATACCCTCCCAGGTTTGACCCAAGTCCAGGTGATTCGAGAAAAAGGAATGGACTTTGCCTCAATTTTGAGAGCGTT
TCTAAGACAAGATCCAGACGTCATTCTAGTGGGTGAGACCCGAGATAAAGAAACGGCTAAAACTGCCATTGAGGCAGCGC
TAACAGGACACCTTGTGCTAACGACTTTGCACACAAATGACGCTGCTAGTGCAGTTGCTCGCCTTGCGGAAATGGAAGTT
GAGCCTTTTATGGTTTCGGCGTCCCTATTGGGTGTTGTTGCCCAGCGTTTGGTTCGGCGAGTATGCTCAGACTGTAAAGT
CCCTTATCAACCTACTCCCACTGAGCTTGGCCGATTTGGGTTATCTGCTTCTCCTGGCACAGAGATTACCTTCTATAAAG
CGAATACTCTCTCAGCTGATGAACTTCAAACCGCTCAAGCTACTGGGCAAACGGTTTGTTCAACTTGTAATGGTATTGGT
TATAAAGGGCGTTGTGGTGTCTATGAAGTCATGCAAATCACTGAAACCCTGCAAACACTAATTACCGAGGGAGCTCCTAC
CGAGCGGATTAAAGAAGTAGCAGTTGAAGAAGGAATGAAAACCCTTCTAGCTTACAGTCTTGAACTAGTTAAGAAAGGGG
ATACTACTCTCGCTGAAGTGGAACGAGTTACCTTTACAGATAGTGGCTTAGAAGCTGAACTTAAGGCTAAGCGCAAAACA
TCTCTAACTTGTCGAGTCTGTGATGCAGATTTAAGACCTGAGTGGTTGGATTGTCCCTATTGCACGACACCAAGATTCCA
AGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(58-180)

Type II/IV secretion system protein (T2SSE)

(233-500)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0CAY6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

63.801

99.251

0.633
  pilB Acinetobacter baumannii D1279779

38.871

92.814

0.361

Multiple sequence alignment