Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   LGMS210922A_RS02295 Genome accession   NZ_AP026069
Coordinates   471780..473585 (+) Length   601 a.a.
NCBI ID   WP_265150334.1    Uniprot ID   A0AA43PEJ2
Organism   Lactococcus garvieae strain MS210922A     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 466780..478585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGMS210922A_RS02275 (LGMS210922A_04420) - 466938..467762 (-) 825 WP_014024379.1 Cof-type HAD-IIB family hydrolase -
  LGMS210922A_RS02280 (LGMS210922A_04430) - 468119..469540 (+) 1422 WP_014024380.1 NCS2 family permease -
  LGMS210922A_RS02285 (LGMS210922A_04440) - 469617..470717 (+) 1101 WP_281162249.1 YdcF family protein -
  LGMS210922A_RS02290 (LGMS210922A_04450) coiA 470792..471796 (+) 1005 WP_265150332.1 competence protein CoiA Machinery gene
  LGMS210922A_RS02295 (LGMS210922A_04460) pepF 471780..473585 (+) 1806 WP_265150334.1 oligoendopeptidase F Regulator
  LGMS210922A_RS02300 (LGMS210922A_04470) - 473585..474268 (+) 684 WP_004258974.1 O-methyltransferase -
  LGMS210922A_RS02305 (LGMS210922A_04480) - 474366..475274 (+) 909 WP_265150336.1 peptidyl-prolyl cis-trans isomerase -
  LGMS210922A_RS02310 (LGMS210922A_04490) alaS 475525..478143 (+) 2619 WP_265150338.1 alanine--tRNA ligase -
  LGMS210922A_RS02315 (LGMS210922A_04500) - 478285..478482 (-) 198 WP_016170988.1 hypothetical protein -

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 69778.40 Da        Isoelectric Point: 4.7631

>NTDB_id=95630 LGMS210922A_RS02295 WP_265150334.1 471780..473585(+) (pepF) [Lactococcus garvieae strain MS210922A]
MAKKRDEIAENLTWDLSTVFENDAAWELELKRVSEKLETVNAYEGHLLDSAQSLLEITEIELEISRAVEKVYVYASMKHD
QDTQVSLYQEYEAKATNLYAKFGESFAFYEPEFLALTPERYESFRREEPKLERYSHMFERLFAKKEHILSPKEEKLLASA
SEIFGAAAETFEIFDNADIELPMIKDEKGQEVQLTHGNYISLMESKERKVRKAAYQALYSNYEQYQHTYAKTLQTNVKVH
NFKAKMRGYHSARQAALSSNFIPEEVYDKLLESVNKYLPLLHRYIKLREQILGLEDDLKMYDVYTPLSDLDYKFNYPEAV
EKAQEVLQILGPEYAHHVKKAFEERWIDVAENSGKRSGAYSGGCYDTNAFMLLNWQETLDDLFTLVHEMGHSMHSTFTRE
NQPYVYGDYPIFLAEIASTTNENILTESLLAASTDEKVRFAILNHWLDGFRGTVFRQSQFAEFEHEIHKADQEGKVLTSE
FMNQLYADLNEKYYGLPARENSEIQYEWARIPHFYYNYYVFQYATGFAAATYLAEAIVKGGTDAREKYLTYLKAGSSDYP
LAVIAQAGVDMEETTYLESAFAVFEKRLIELEKLVEKGAHL

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=95630 LGMS210922A_RS02295 WP_265150334.1 471780..473585(+) (pepF) [Lactococcus garvieae strain MS210922A]
ATGGCAAAAAAACGTGATGAAATAGCTGAAAACTTAACTTGGGATTTGTCAACTGTATTTGAAAATGATGCCGCCTGGGA
ACTTGAACTTAAAAGAGTTTCAGAAAAATTAGAGACGGTAAACGCTTATGAAGGACATCTTCTAGATAGCGCTCAAAGCT
TATTAGAAATCACGGAAATTGAGCTAGAAATCTCGCGTGCTGTGGAAAAGGTTTATGTCTATGCCTCAATGAAGCATGAC
CAAGATACGCAAGTGAGCCTTTACCAAGAATACGAAGCCAAAGCCACAAATTTATATGCCAAGTTTGGTGAAAGCTTTGC
TTTTTATGAGCCGGAATTTTTAGCTTTAACGCCAGAACGTTATGAAAGTTTTAGAAGAGAAGAACCTAAACTGGAACGCT
ATTCCCATATGTTTGAACGCCTTTTTGCTAAAAAGGAGCATATTTTAAGTCCAAAAGAGGAAAAACTTTTAGCCAGTGCG
AGTGAGATTTTTGGTGCTGCGGCCGAAACCTTTGAAATTTTTGACAATGCTGATATTGAACTTCCGATGATAAAAGATGA
AAAAGGACAGGAAGTACAGCTGACACATGGCAATTATATCTCGCTCATGGAAAGTAAGGAGCGTAAGGTACGAAAGGCAG
CTTATCAAGCCCTTTATAGCAACTATGAACAGTATCAACACACTTATGCTAAAACGCTACAAACAAATGTAAAAGTCCAT
AATTTTAAGGCAAAGATGCGGGGCTACCATTCAGCTCGTCAGGCAGCTTTATCAAGTAATTTTATTCCGGAAGAAGTCTA
CGATAAGCTCCTAGAGTCTGTCAATAAATATTTGCCTCTTTTACATCGCTATATTAAATTGCGTGAACAGATTTTAGGTT
TAGAGGACGATTTAAAGATGTATGATGTTTATACACCCTTATCGGACTTGGATTATAAGTTTAACTACCCCGAGGCAGTA
GAGAAAGCCCAAGAAGTTCTTCAAATTTTGGGGCCTGAATACGCGCACCATGTAAAAAAAGCTTTTGAGGAGCGTTGGAT
TGATGTGGCTGAAAATAGCGGTAAACGTTCGGGTGCTTACTCAGGTGGATGCTATGATACAAATGCTTTTATGCTGTTAA
ACTGGCAAGAAACTTTGGATGATTTGTTCACCTTAGTACATGAAATGGGTCACAGTATGCATTCCACATTTACACGGGAA
AATCAACCTTATGTTTATGGGGACTATCCAATCTTTCTTGCAGAAATTGCTTCAACAACCAATGAGAATATCTTGACTGA
AAGTTTACTTGCGGCGTCGACAGATGAAAAAGTGCGTTTTGCTATTTTAAACCACTGGCTTGATGGTTTCCGTGGCACTG
TTTTTCGTCAAAGTCAATTTGCTGAATTTGAACATGAAATACATAAAGCTGATCAAGAAGGAAAAGTTTTGACCAGTGAA
TTCATGAACCAACTTTATGCAGATCTTAATGAAAAATACTACGGTTTACCCGCCCGGGAAAATTCTGAAATTCAGTATGA
GTGGGCCCGCATTCCGCACTTCTATTACAACTATTATGTCTTCCAATATGCTACTGGCTTTGCCGCAGCAACTTATCTTG
CTGAGGCTATCGTCAAGGGTGGGACAGATGCGCGTGAAAAATACCTCACTTATCTCAAAGCCGGATCAAGTGATTATCCC
CTGGCTGTGATTGCTCAAGCAGGTGTGGATATGGAAGAAACAACATATTTAGAATCAGCTTTTGCTGTTTTTGAAAAACG
TTTAATTGAGCTAGAAAAATTAGTCGAAAAAGGAGCCCATTTATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(112-180)

Peptidase family M3 (Peptidase_M3)

(202-582)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

65.058

100

0.651

Multiple sequence alignment