NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	WHO72_RS03460	Genome accession	NZ_CP148126
Coordinates	758199..759065 (-)	Length	288 a.a.
NCBI ID	WP_010951890.1	Uniprot ID	Q88Q64
Organism	Pseudomonas putida isolate FELIX_MS547
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 753199..764065

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WHO72_RS03425	-	753671..754966 (-)	1296	WP_003247549.1	NAD(P)/FAD-dependent oxidoreductase	-
WHO72_RS03430	-	755051..755224 (-)	174	WP_008091951.1	DUF3094 domain-containing protein	-
WHO72_RS03435	-	755357..755986 (-)	630	WP_003247546.1	DUF1780 domain-containing protein	-
WHO72_RS03440	-	756042..756473 (-)	432	WP_049588119.1	hypothetical protein	-
WHO72_RS03445	-	756560..757267 (+)	708	WP_049588121.1	energy-coupling factor ABC transporter permease	-
WHO72_RS03450	yacG	757379..757579 (-)	201	WP_010951889.1	DNA gyrase inhibitor YacG	-
WHO72_RS03455	coaE	757576..758199 (-)	624	WP_004575721.1	dephospho-CoA kinase	-
WHO72_RS03460	pilD	758199..759065 (-)	867	WP_010951890.1	A24 family peptidase	Machinery gene
WHO72_RS03465	-	759068..760276 (-)	1209	WP_010951891.1	type II secretion system F family protein	-
WHO72_RS03470	-	760493..760903 (+)	411	WP_010951892.1	pilin	-
WHO72_RS03480	ltrA	761550..762971 (+)	1422	WP_049587292.1	group II intron reverse transcriptase/maturase	-
WHO72_RS03485	-	763245..763985 (-)	741	WP_010951894.1	cold shock domain-containing protein	-

Sequence

Protein

Download Length: 288 a.a. Molecular weight: 31830.86 Da Isoelectric Point: 7.5986

                                >NTDB_id=951357 WHO72_RS03460 WP_010951890.1 758199..759065(-) (pilD) [Pseudomonas putida isolate FELIX_MS547]

MTLWALLAEQPAYFLTLATVLGLLVGSFINVLVYRLPIMLERQWQREAQEVLGLPTTQHARFDLCLPASRCPHCAHRIRA

WENIPVISYLALGGRCSSCKNRISLRYPVVEVASALLSLVVAWRFGASVEALVALPLTWCLLALSLIDADHQLLPDVLVL

PTMWLGLIVNAFGIHVPLADALWGAVAGYLSLWTVYWVFRLVTGKEGMGYGDFKLMALIGAWGGWQVLPLTLLLSSVVGA

LFGLCLLRFRRDAMGTAIPFGPYLAIAGWIAVLWGDEIYASYMQLLGY

Nucleotide

Download Length: 867 bp

                                >NTDB_id=951357 WHO72_RS03460 WP_010951890.1 758199..759065(-) (pilD) [Pseudomonas putida isolate FELIX_MS547]

ATGACTTTATGGGCTTTGCTGGCTGAACAGCCAGCGTATTTCCTCACCCTTGCCACGGTGCTCGGCCTGCTGGTGGGCAG

CTTTATCAACGTGCTGGTGTATCGCCTGCCGATCATGCTGGAGCGGCAATGGCAGCGTGAGGCGCAAGAGGTATTGGGGC

TGCCGACCACGCAGCACGCGCGTTTCGACCTGTGCCTGCCCGCCTCCCGGTGCCCACACTGCGCGCACCGGATTCGTGCG

TGGGAGAACATTCCGGTCATCAGCTACCTGGCCTTGGGCGGGCGCTGTTCGTCCTGCAAAAACCGCATCAGCCTGCGTTA

CCCGGTAGTGGAAGTGGCCAGCGCGCTGCTGTCGCTGGTAGTGGCCTGGCGCTTCGGCGCTTCGGTAGAGGCGTTGGTGG

CTCTGCCGCTGACCTGGTGCCTGCTGGCGCTGAGCCTGATCGATGCGGACCACCAGCTGCTGCCGGATGTGCTGGTGCTG

CCGACCATGTGGCTAGGGCTGATCGTCAATGCCTTTGGCATCCATGTGCCCCTGGCCGACGCACTGTGGGGCGCAGTGGC

CGGGTACCTCAGCCTGTGGACGGTGTACTGGGTGTTCAGGCTGGTCACCGGCAAGGAAGGCATGGGCTATGGCGATTTCA

AGCTGATGGCGCTGATCGGGGCGTGGGGGGGTTGGCAGGTGCTGCCGTTGACGCTGCTGTTGTCGTCGGTGGTGGGGGCG

CTGTTTGGCCTGTGCCTGCTGCGTTTTCGCCGGGATGCCATGGGCACGGCAATCCCGTTTGGCCCTTATCTGGCAATTGC

GGGGTGGATTGCCGTGCTCTGGGGTGATGAAATATACGCCTCATACATGCAACTGCTTGGATACTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-125)

Type IV leader peptidase family (Peptidase_A24)

(137-244)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q88Q64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Vibrio cholerae strain A1552	52.347	96.181	0.503
pilD	Acinetobacter baumannii D1279779	49.822	97.569	0.486
pilD	Acinetobacter nosocomialis M2	49.11	97.569	0.479
pilD	Vibrio campbellii strain DS40M4	48.913	95.833	0.469
pilD	Neisseria gonorrhoeae MS11	48.327	93.403	0.451

Detailed information NTG0951357