Detailed information    

insolico Bioinformatically predicted

Overview


Name   phrF   Type   Regulator
Locus tag   WHO59_RS20220 Genome accession   NZ_CP148120
Coordinates   3846426..3846545 (+) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis isolate FELIX_MS512     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context


Location: 3841426..3851545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO59_RS20195 albG 3841590..3842291 (+) 702 WP_003243391.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  WHO59_RS20200 ywhL 3842297..3843673 (-) 1377 WP_003244415.1 YncE family protein -
  WHO59_RS20205 ywhK 3843712..3845067 (-) 1356 WP_003227552.1 YncE family protein -
  WHO59_RS20210 - 3845064..3845294 (-) 231 WP_009968328.1 hypothetical protein -
  WHO59_RS20215 rapF 3845297..3846442 (+) 1146 WP_003227549.1 response regulator aspartate phosphatase RapF Regulator
  WHO59_RS20220 phrF 3846426..3846545 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  WHO59_RS20225 ywhH 3846644..3847117 (+) 474 WP_003227547.1 YbaK/EbsC family protein -
  WHO59_RS20230 speB 3847149..3848021 (-) 873 WP_003227545.1 agmatinase -
  WHO59_RS20235 speE 3848082..3848912 (-) 831 WP_003227543.1 spermidine synthase -
  WHO59_RS20240 pbpG 3849114..3851189 (+) 2076 WP_003227540.1 transglycosylase domain-containing protein -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=951179 WHO59_RS20220 WP_009968329.1 3846426..3846545(+) (phrF) [Bacillus subtilis isolate FELIX_MS512]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=951179 WHO59_RS20220 WP_009968329.1 3846426..3846545(+) (phrF) [Bacillus subtilis isolate FELIX_MS512]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1

  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487