Detailed information NTG0951086

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACHP5H_RS21230
• Genome accession: NZ_CP172194
• Coordinates: 4475754..4477952 (+)
• Length: 732 a.a.
• NCBI ID: WP_021557385.1
• Uniprot ID: -
• Organism: Escherichia coli strain GF66.1
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4470754..4482952

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACHP5H_RS21200 (ACHP5H_21200)	-	4471389..4472081 (+)	693	Protein_4145	IS1 family transposase	-
ACHP5H_RS21205 (ACHP5H_21205)	-	4472151..4472474 (-)	324	WP_021557388.1	RHS element protein dsORF-f3	-
ACHP5H_RS21210 (ACHP5H_21210)	-	4472476..4473510 (-)	1035	Protein_4147	RHS repeat-associated core domain-containing protein	-
ACHP5H_RS21215 (ACHP5H_21215)	-	4473517..4474653 (-)	1137	Protein_4148	ISAs1-like element ISEc1 family transposase	-
ACHP5H_RS21220 (ACHP5H_21220)	-	4474787..4475179 (-)	393	Protein_4149	DUF6531 domain-containing protein	-
ACHP5H_RS21225 (ACHP5H_21225)	rpmE	4475339..4475551 (-)	213	WP_000710769.1	50S ribosomal protein L31	-
ACHP5H_RS21230 (ACHP5H_21230)	priA	4475754..4477952 (+)	2199	WP_021557385.1	primosomal protein N'	Machinery gene
ACHP5H_RS21235 (ACHP5H_21235)	cytR	4478108..4479133 (+)	1026	WP_000644904.1	DNA-binding transcriptional regulator CytR	Regulator
ACHP5H_RS21240 (ACHP5H_21240)	ftsN	4479225..4480184 (+)	960	WP_000068837.1	cell division protein FtsN	-
ACHP5H_RS21245 (ACHP5H_21245)	hslV	4480277..4480807 (+)	531	WP_000208242.1	ATP-dependent protease subunit HslV	-
ACHP5H_RS21250 (ACHP5H_21250)	hslU	4480817..4482148 (+)	1332	WP_001293341.1	HslU--HslV peptidase ATPase subunit	-

Sequence

Protein

• Length: 732 a.a.
• Molecular weight: 81631.88 Da
• Isoelectric Point: 9.0294

>NTDB_id=951086 ACHP5H_RS21230 WP_021557385.1 4475754..4477952(+) (priA) [Escherichia coli strain GF66.1]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGIVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide

• Length: 2199 bp

>NTDB_id=951086 ACHP5H_RS21230 WP_021557385.1 4475754..4477952(+) (priA) [Escherichia coli strain GF66.1]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCTGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGATTGTGGTATCAGTTAGCGATGCCAGCG
AACTGCCGCTCAATGAGCTAAAAGCGGTAGTCGAAGTGCTGGATAGTGAGCCGGTGTTTACTCACTCCGTCTGGCGATTG
CTGCTATGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTGAACAGCCTGAAAC
GTTCGCCCAAGCAACAACAGGCGCTGGCAGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTTGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAACGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGTGCGG
CGGATACCTTCTCTGCCTGGCTGCTGGCGGGCGTTACAGGTTCCGGTAAAACAGAGGTTTATTTGAGCGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGCTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGTTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTTTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACACCCGCACTGGAAACGTTATGCAACGTCCAGCAGAAAAAATACCGCC
TGCTACGCCTGACGCGTCGGGCGGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCGGGGCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCGACAACCAGGTTATCCTCTTTCTTAATCG
TCGGGGTTTTGCGCCTGCGCTGCTGTGCCACGACTGCGGCTGGATAGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGCCAACGCCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGTTATTCCCTGGTGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGAGCGCTGGAACAACAACTGGCGGAAGTGCATCGCGGCGGCGCGA
GGATCTTGATTGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTAGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTTGCCCAGCTATACACCCAGGTAGCAGGCCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCATCATCCGGAACACCCGCTATTGCAAACGTTGCTCTATAAAGGCT
ACGACGCTTTTGCCGAACAGGCACTGGCTGAGCGACGCATGATGCAACTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCATTGTTCCTGCAGCAACTGCGTAATCTGATCCTCTCCAGCCCGCTGGCAGA
CGACAAATTGTGGGTTCTCGGTCCGGTTCCGGCACTGGCACCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAACGGTACGCTGGCACTCATCAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTTGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (522-589)

• Primosomal protein N C-terminal domain (PriA_C) (632-729)

• DEAD/DEAH box helicase (DEAD) (203-362)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (445-471)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-101)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	44.912	100	0.452