Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACHP5I_RS27295 Genome accession   NZ_CP172185
Coordinates   5502063..5504261 (+) Length   732 a.a.
NCBI ID   WP_096990206.1    Uniprot ID   -
Organism   Escherichia coli strain GF60     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5497063..5509261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHP5I_RS27275 (ACHP5I_27275) metB 5499165..5500325 (-) 1161 WP_001295694.1 cystathionine gamma-synthase -
  ACHP5I_RS27280 (ACHP5I_27280) metJ 5500602..5500919 (+) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  ACHP5I_RS27285 (ACHP5I_27285) yiiX 5500979..5501587 (+) 609 WP_000702301.1 YiiX family permuted papain-like enzyme -
  ACHP5I_RS27290 (ACHP5I_27290) rpmE 5501648..5501860 (-) 213 WP_000710769.1 50S ribosomal protein L31 -
  ACHP5I_RS27295 (ACHP5I_27295) priA 5502063..5504261 (+) 2199 WP_096990206.1 primosomal protein N' Machinery gene
  ACHP5I_RS27300 (ACHP5I_27300) cytR 5504417..5505442 (+) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  ACHP5I_RS27305 (ACHP5I_27305) ftsN 5505534..5506493 (+) 960 WP_000068834.1 cell division protein FtsN -
  ACHP5I_RS27310 (ACHP5I_27310) hslV 5506586..5507116 (+) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  ACHP5I_RS27315 (ACHP5I_27315) hslU 5507126..5508457 (+) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81704.07 Da        Isoelectric Point: 9.3662

>NTDB_id=951006 ACHP5I_RS27295 WP_096990206.1 5502063..5504261(+) (priA) [Escherichia coli strain GF60]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEKGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGTIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHSSRVRLQHIINGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=951006 ACHP5I_RS27295 WP_096990206.1 5502063..5504261(+) (priA) [Escherichia coli strain GF60]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTATCAGTGAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGATGTTGAGCCGGTATTTACTCACTCCGTCTGGCGATTA
CTGCTTTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAAAAAGGCCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCGCCAAAGCAACAACAGGCACTGGCGGCATTGCGGCAAGGCAAAATCTGGCGCGACCAAGTTGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCACTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGTGACTGGCG
AAAGAATTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACTGAACAGGCCACCGCCGTTGGCACAATTCATAGTGCGG
CGGATACCTTCTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACGCCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTAGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTTATTGAT
GAAGAGCACGACAGTTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACGCCCGCGCTGGAAACGTTGTGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACCCGTCGGGCGGGCAATGCGCGGCCGGCAATTCAACATGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCTGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCAACAATCAGGTTATCCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGCCTTGCTGTGCCACGACTGCGGCTGGATTGCCGAATGCCCACGTTGTGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGCCAACGCCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTGCCGGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGCTGTTCCCCGGCGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCGGAAGTGCATCGCGGCGGCGCGA
GGATCTTGATTGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTAGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCCCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAGCGACGGATGATGCAGCTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCATGCGCCGCTGTTCCTGCAACAACTGCGTAATCTGATCCTCTCCAGCCCGCTGGCAGA
CGACAAACTGTGGGTTCTCGGTCCGGTTCCGGCGCTGGCACCTAAACGTGGCGGTCGCTGGCGCTGGCAAATTTTGTTGC
AGCACTCTTCCCGCGTGCGCTTACAGCACATCATCAATGGTACGCTGGCACTTATTAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTCGATCCCATCGAGGGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(203-362)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.776

100

0.451