Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   WHO32_RS20245 Genome accession   NZ_CP148112
Coordinates   3846748..3846867 (+) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis isolate FELIX_MS504     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 3841748..3851867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO32_RS20220 albG 3841912..3842613 (+) 702 WP_003243391.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  WHO32_RS20225 ywhL 3842619..3843995 (-) 1377 WP_003244415.1 YncE family protein -
  WHO32_RS20230 ywhK 3844034..3845389 (-) 1356 WP_003227552.1 YncE family protein -
  WHO32_RS20235 - 3845386..3845616 (-) 231 WP_009968328.1 hypothetical protein -
  WHO32_RS20240 rapF 3845619..3846764 (+) 1146 WP_003227549.1 response regulator aspartate phosphatase RapF Regulator
  WHO32_RS20245 phrF 3846748..3846867 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  WHO32_RS20250 ywhH 3846966..3847439 (+) 474 WP_003227547.1 YbaK/EbsC family protein -
  WHO32_RS20255 speB 3847471..3848343 (-) 873 WP_003227545.1 agmatinase -
  WHO32_RS20260 speE 3848404..3849234 (-) 831 WP_003227543.1 spermidine synthase -
  WHO32_RS20265 pbpG 3849436..3851511 (+) 2076 WP_003227540.1 transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=950877 WHO32_RS20245 WP_009968329.1 3846748..3846867(+) (phrF) [Bacillus subtilis isolate FELIX_MS504]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=950877 WHO32_RS20245 WP_009968329.1 3846748..3846867(+) (phrF) [Bacillus subtilis isolate FELIX_MS504]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1
  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487