Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   WHO32_RS13430 Genome accession   NZ_CP148112
Coordinates   2558594..2559664 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis isolate FELIX_MS504     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2553594..2564664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO32_RS13385 tapA 2554073..2554834 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  WHO32_RS13390 yqzG 2555106..2555432 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  WHO32_RS13395 spoIIT 2555474..2555653 (-) 180 WP_003230176.1 YqzE family protein -
  WHO32_RS13400 comGG 2555724..2556098 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  WHO32_RS13405 comGF 2556099..2556482 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  WHO32_RS13410 comGE 2556508..2556855 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  WHO32_RS13415 comGD 2556839..2557270 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  WHO32_RS13420 comGC 2557260..2557556 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  WHO32_RS13425 comGB 2557570..2558607 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  WHO32_RS13430 comGA 2558594..2559664 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  WHO32_RS13435 corA 2560076..2561029 (-) 954 WP_004399136.1 magnesium transporter CorA -
  WHO32_RS13440 yqhB 2561172..2562500 (+) 1329 WP_009967730.1 hemolysin family protein -
  WHO32_RS13445 rsbRD 2562553..2563389 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -
  WHO32_RS13455 mgsR 2563613..2563993 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  WHO32_RS13460 yqgY 2564225..2564470 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=950848 WHO32_RS13430 WP_004399124.1 2558594..2559664(-) (comGA) [Bacillus subtilis isolate FELIX_MS504]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=950848 WHO32_RS13430 WP_004399124.1 2558594..2559664(-) (comGA) [Bacillus subtilis isolate FELIX_MS504]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGTTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1