Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   WGK59_RS03475 Genome accession   NZ_CP148002
Coordinates   682393..682932 (-) Length   179 a.a.
NCBI ID   WP_015701598.1    Uniprot ID   -
Organism   Haemophilus influenzae strain AR2427     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 677393..687932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WGK59_RS03445 (WGK59_03445) fdx 677796..678137 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  WGK59_RS03450 (WGK59_03450) iscX 678137..678331 (+) 195 WP_005630102.1 Fe-S cluster assembly protein IscX -
  WGK59_RS03455 (WGK59_03455) - 678382..678996 (-) 615 WP_061659667.1 YfgM family protein -
  WGK59_RS03460 (WGK59_03460) hisS 679014..680285 (-) 1272 WP_013525573.1 histidine--tRNA ligase -
  WGK59_RS03465 (WGK59_03465) ispG 680295..681401 (-) 1107 WP_015701600.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  WGK59_RS03470 (WGK59_03470) - 681410..682321 (-) 912 WP_015701599.1 RodZ domain-containing protein -
  WGK59_RS03475 (WGK59_03475) pilF/pilF2 682393..682932 (-) 540 WP_015701598.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  WGK59_RS03480 (WGK59_03480) - 683033..684184 (-) 1152 WP_015701597.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  WGK59_RS03485 (WGK59_03485) - 684431..685309 (+) 879 WP_005649130.1 D-alanyl-D-alanine carboxypeptidase family protein -
  WGK59_RS03490 (WGK59_03490) - 685488..686369 (+) 882 WP_015701596.1 metal ABC transporter substrate-binding protein -
  WGK59_RS03495 (WGK59_03495) - 686369..687289 (+) 921 WP_015701595.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20585.25 Da        Isoelectric Point: 8.6194

>NTDB_id=949978 WGK59_RS03475 WP_015701598.1 682393..682932(-) (pilF/pilF2) [Haemophilus influenzae strain AR2427]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQTDTFENIALCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=949978 WGK59_RS03475 WP_015701598.1 682393..682932(-) (pilF/pilF2) [Haemophilus influenzae strain AR2427]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCAGCATCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAACAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGCGCGGAAAAATTCAATAGTCTAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(73-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.089

100

0.961
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453