Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   V7S31_RS13550 Genome accession   NZ_CP147655
Coordinates   2793087..2793701 (-) Length   204 a.a.
NCBI ID   WP_020956097.1    Uniprot ID   -
Organism   Bacillus velezensis strain M1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2788087..2798701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V7S31_RS13520 rpsT 2788390..2788656 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  V7S31_RS13525 holA 2788673..2789714 (-) 1042 Protein_2616 DNA polymerase III subunit delta -
  V7S31_RS13530 - 2789772..2789905 (-) 134 Protein_2617 hypothetical protein -
  V7S31_RS13535 - 2789946..2790080 (+) 135 WP_003152870.1 YqzM family protein -
  V7S31_RS13540 comEC 2790099..2792450 (-) 2352 WP_032866696.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  V7S31_RS13545 - 2792451..2793020 (-) 570 WP_007408264.1 ComE operon protein 2 -
  V7S31_RS13550 comEA 2793087..2793701 (-) 615 WP_020956097.1 helix-hairpin-helix domain-containing protein Machinery gene
  V7S31_RS13555 comER 2793760..2794581 (+) 822 WP_020956098.1 late competence protein ComER -
  V7S31_RS13560 - 2794651..2795388 (-) 738 WP_015240247.1 class I SAM-dependent methyltransferase -
  V7S31_RS13565 rsfS 2795385..2795741 (-) 357 WP_007408260.1 ribosome silencing factor -
  V7S31_RS13570 yqeK 2795759..2796319 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  V7S31_RS13575 - 2796309..2796878 (-) 570 WP_039063329.1 nicotinate-nucleotide adenylyltransferase -
  V7S31_RS13580 yhbY 2796889..2797179 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  V7S31_RS13585 aroE 2797173..2798015 (-) 843 WP_025285019.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22251.45 Da        Isoelectric Point: 6.4940

>NTDB_id=947878 V7S31_RS13550 WP_020956097.1 2793087..2793701(-) (comEA) [Bacillus velezensis strain M1]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=947878 V7S31_RS13550 WP_020956097.1 2793087..2793701(-) (comEA) [Bacillus velezensis strain M1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.071

100

0.363