Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WBM82_RS02460 Genome accession   NZ_CP147494
Coordinates   364898..365347 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain YT1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 359898..370347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WBM82_RS02420 (WBM82_02425) - 360221..360418 (+) 198 WP_119122841.1 hypothetical protein -
  WBM82_RS02425 (WBM82_02430) yckC 360437..360892 (+) 456 WP_029727075.1 RDD family protein -
  WBM82_RS02430 (WBM82_02435) yckD 360974..361306 (+) 333 WP_248719472.1 YckD family protein -
  WBM82_RS02435 (WBM82_02440) - 361434..361856 (+) 423 WP_174616579.1 MarR family transcriptional regulator -
  WBM82_RS02440 (WBM82_02445) - 361844..362185 (+) 342 WP_032724911.1 DUF3147 family protein -
  WBM82_RS02445 (WBM82_02450) - 362204..362932 (+) 729 WP_160221337.1 AAA family ATPase -
  WBM82_RS02450 (WBM82_02455) bglC 363003..364436 (+) 1434 WP_029727076.1 6-phospho-beta-glucosidase -
  WBM82_RS02455 (WBM82_02460) nin/comJ 364473..364871 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  WBM82_RS02460 (WBM82_02465) nucA/comI 364898..365347 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  WBM82_RS02465 (WBM82_02470) tlpC 365515..367236 (-) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  WBM82_RS02470 (WBM82_02475) hxlB 367347..367904 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  WBM82_RS02475 (WBM82_02480) hxlA 367910..368542 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  WBM82_RS02480 (WBM82_02485) hxlR 368776..369138 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=946389 WBM82_RS02460 WP_003234608.1 364898..365347(-) (nucA/comI) [Bacillus subtilis strain YT1]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=946389 WBM82_RS02460 WP_003234608.1 364898..365347(-) (nucA/comI) [Bacillus subtilis strain YT1]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98