Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   WBI56_RS12465 Genome accession   NZ_CP147492
Coordinates   2424278..2425348 (-) Length   356 a.a.
NCBI ID   WP_015714258.1    Uniprot ID   -
Organism   Bacillus subtilis strain MC4-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2419278..2430348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WBI56_RS12420 (WBI56_12420) tapA 2419756..2420517 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  WBI56_RS12425 (WBI56_12425) yqzG 2420789..2421115 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  WBI56_RS12430 (WBI56_12430) spoIIT 2421157..2421336 (-) 180 WP_029726723.1 YqzE family protein -
  WBI56_RS12435 (WBI56_12435) comGG 2421408..2421782 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  WBI56_RS12440 (WBI56_12440) comGF 2421783..2422166 (-) 384 WP_046160582.1 ComG operon protein ComGF Machinery gene
  WBI56_RS12445 (WBI56_12445) comGE 2422192..2422539 (-) 348 WP_046381178.1 ComG operon protein 5 Machinery gene
  WBI56_RS12450 (WBI56_12450) comGD 2422523..2422954 (-) 432 WP_046381179.1 comG operon protein ComGD Machinery gene
  WBI56_RS12455 (WBI56_12455) comGC 2422944..2423240 (-) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  WBI56_RS12460 (WBI56_12460) comGB 2423254..2424291 (-) 1038 WP_338730579.1 comG operon protein ComGB Machinery gene
  WBI56_RS12465 (WBI56_12465) comGA 2424278..2425348 (-) 1071 WP_015714258.1 competence protein ComGA Machinery gene
  WBI56_RS12470 (WBI56_12470) corA 2425760..2426713 (-) 954 WP_338730580.1 magnesium transporter CorA -
  WBI56_RS12475 (WBI56_12475) yqhB 2426856..2428184 (+) 1329 WP_003230150.1 hemolysin family protein -
  WBI56_RS12480 (WBI56_12480) rsbRD 2428237..2429073 (-) 837 WP_015714260.1 RsbT co-antagonist protein RsbRD -
  WBI56_RS12490 (WBI56_12490) mgsR 2429297..2429677 (-) 381 WP_014477337.1 transcriptional regulator MgsR -
  WBI56_RS12495 (WBI56_12495) yqgY 2429909..2430154 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40488.67 Da        Isoelectric Point: 9.0175

>NTDB_id=946352 WBI56_RS12465 WP_015714258.1 2424278..2425348(-) (comGA) [Bacillus subtilis strain MC4-2]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=946352 WBI56_RS12465 WP_015714258.1 2424278..2425348(-) (comGA) [Bacillus subtilis strain MC4-2]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTCTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCACAGGTCTGAAAGCAATTTTGCGCCATGATCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATTTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCCATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGCCAGTCAC
GAAATACCAGGAGAGCTAGTGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATTCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997