Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WBI56_RS01980 Genome accession   NZ_CP147492
Coordinates   370673..371122 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain MC4-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365673..376122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WBI56_RS01940 (WBI56_01940) - 365996..366191 (+) 196 Protein_338 hypothetical protein -
  WBI56_RS01945 (WBI56_01945) yckC 366210..366665 (+) 456 WP_024572178.1 RDD family protein -
  WBI56_RS01950 (WBI56_01950) yckD 366747..367079 (+) 333 WP_042977218.1 YckD family protein -
  WBI56_RS01955 (WBI56_01955) - 367207..367629 (+) 423 WP_338732227.1 MarR family transcriptional regulator -
  WBI56_RS01960 (WBI56_01960) - 367617..367958 (+) 342 WP_032724911.1 DUF3147 family protein -
  WBI56_RS01965 (WBI56_01965) - 367977..368705 (+) 729 WP_338732229.1 AAA family ATPase -
  WBI56_RS01970 (WBI56_01970) bglC 368778..370211 (+) 1434 WP_338732231.1 6-phospho-beta-glucosidase -
  WBI56_RS01975 (WBI56_01975) nin/comJ 370248..370646 (-) 399 WP_257433798.1 DNA-entry nuclease inhibitor Regulator
  WBI56_RS01980 (WBI56_01980) nucA/comI 370673..371122 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  WBI56_RS01985 (WBI56_01985) tlpC 371290..373011 (-) 1722 WP_338732236.1 methyl-accepting chemotaxis protein TlpC -
  WBI56_RS01990 (WBI56_01990) hxlB 373122..373679 (-) 558 WP_338732238.1 6-phospho-3-hexuloisomerase -
  WBI56_RS01995 (WBI56_01995) hxlA 373685..374317 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  WBI56_RS02000 (WBI56_02000) hxlR 374549..374911 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=946310 WBI56_RS01980 WP_014475770.1 370673..371122(-) (nucA/comI) [Bacillus subtilis strain MC4-2]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=946310 WBI56_RS01980 WP_014475770.1 370673..371122(-) (nucA/comI) [Bacillus subtilis strain MC4-2]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAAGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987